A novel biosensor for investigation of levofloxacin–DNA interactions: A voltammetric biosensor and docking study

Fatih Donmez; Burak Kuzu; Hasret Subak

doi:10.12991/jrespharm.1693336

A novel biosensor for investigation of levofloxacin–DNA interactions: A voltammetric biosensor and docking study

Abstract

This work aims to develop a voltammetric biosensor to analyze the interaction between the quinolone antibiotic levofloxacin (LVX) and DNA. The development steps include defining the electrochemical behaviors of LVX, the DNA-LVX interaction, and molecular docking of DNA-LVX. The exploited docking study determines the interaction mechanism of the binding region of the DNA and LVX. The peak currents of DNA guanine oxidation signals in the presence and absence of LVX were determined by square wave voltammetry using a pencil electrode. In addition, LVX peak currents were evaluated alone, and the interaction between DNA and LVX was determined electrochemically. LVX was determined in the range of 0.5-20 µg/mL. Under the optimum conditions, the LOD value 0.0018 µg/mL was detected for LVX. The effects, such as time, pH, and concentration, affecting the interaction with DNA were examined in the study, and the results were given. The studies were compared with molecular docking, and the data obtained about DNA-drug interactions were compared and characterized.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Analytical Chemistry

Journal Section

Research Article

Authors

Fatih Donmez
Türkiye

Burak Kuzu
0000-0002-7305-7177
Türkiye

Hasret Subak ^*
0000-0003-0100-2529
Türkiye

Publication Date

March 15, 2026

Submission Date

May 6, 2025

Acceptance Date

June 18, 2025

Published in Issue

Year 2026 Volume: 30 Number: 2

DOI

https://doi.org/10.12991/jrespharm.1693336

IZ

https://izlik.org/JA48FM97AL

Cite

RIS / Bibtex

APA

Donmez, F., Kuzu, B., & Subak, H. (2026). A novel biosensor for investigation of levofloxacin–DNA interactions: A voltammetric biosensor and docking study. Journal of Research in Pharmacy, 30(2), 629-643. https://doi.org/10.12991/jrespharm.1693336

AMA

1.Donmez F, Kuzu B, Subak H. A novel biosensor for investigation of levofloxacin–DNA interactions: A voltammetric biosensor and docking study. J. Res. Pharm. 2026;30(2):629-643. doi:10.12991/jrespharm.1693336

Chicago

Donmez, Fatih, Burak Kuzu, and Hasret Subak. 2026. “A Novel Biosensor for Investigation of Levofloxacin–DNA Interactions: A Voltammetric Biosensor and Docking Study”. Journal of Research in Pharmacy 30 (2): 629-43. https://doi.org/10.12991/jrespharm.1693336.

EndNote

Donmez F, Kuzu B, Subak H (March 1, 2026) A novel biosensor for investigation of levofloxacin–DNA interactions: A voltammetric biosensor and docking study. Journal of Research in Pharmacy 30 2 629–643.

IEEE

[1]F. Donmez, B. Kuzu, and H. Subak, “A novel biosensor for investigation of levofloxacin–DNA interactions: A voltammetric biosensor and docking study”, J. Res. Pharm., vol. 30, no. 2, pp. 629–643, Mar. 2026, doi: 10.12991/jrespharm.1693336.

ISNAD

Donmez, Fatih - Kuzu, Burak - Subak, Hasret. “A Novel Biosensor for Investigation of Levofloxacin–DNA Interactions: A Voltammetric Biosensor and Docking Study”. Journal of Research in Pharmacy 30/2 (March 1, 2026): 629-643. https://doi.org/10.12991/jrespharm.1693336.

JAMA

1.Donmez F, Kuzu B, Subak H. A novel biosensor for investigation of levofloxacin–DNA interactions: A voltammetric biosensor and docking study. J. Res. Pharm. 2026;30:629–643.

MLA

Donmez, Fatih, et al. “A Novel Biosensor for Investigation of Levofloxacin–DNA Interactions: A Voltammetric Biosensor and Docking Study”. Journal of Research in Pharmacy, vol. 30, no. 2, Mar. 2026, pp. 629-43, doi:10.12991/jrespharm.1693336.

Vancouver

1.Fatih Donmez, Burak Kuzu, Hasret Subak. A novel biosensor for investigation of levofloxacin–DNA interactions: A voltammetric biosensor and docking study. J. Res. Pharm. 2026 Mar. 1;30(2):629-43. doi:10.12991/jrespharm.1693336