Purification of acethylcholinesterase from the mollusc Mytilus galloprovincialis Lam. and investigation of its kinetic properties

Servet Duranay; Sezin Anıl; Gözde Hasbal; Nurten Ozsoy

Purification of acethylcholinesterase from the mollusc Mytilus galloprovincialis Lam. and investigation of its kinetic properties

Abstract

The role of acetylcholinesterase in terminating acetylcholine-mediated neurotransmission made it the focus of intense research. In this study the haemolymph Acetylcholinesterase (AChE) from the mollusc Mytilus galloprovincialis was purified to homogeneity by (NH4)2SO4 fractional precipitation and affinity chromatography on edrophonium-Sepharose 6B. The enzyme was purified approximatedly 13 fold over the crude extract and was obtained in 3 % yield. The specific activity of purified enzyme was 3 U/mg protein. It had an optimum pH of 7.5 and showed optimal activity at 35oC. Km and Vmax for acetylthiocholine iodide were 1.3 mM and 0.188 mM/mg/min, respectively. The purified enzyme migrated as a 51 000 dalton band during polyacrylamide gel electrophoresis under denaturing and reducing conditions. Three methoxyflavones were examined in order to evaluate their potential as anti-Alzheimer’s Disease (AD) agents. All of the compounds were shown to be potent AChE inhibitors. Therefore, these compounds may have great value in the development of therapeutic and preventive agents for AD.

Keywords

References

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Details

Primary Language

English

Subjects

Pharmaceutical Biochemistry, Pharmacognosy

Journal Section

Research Article

Authors

Servet Duranay ^* This is me
Türkiye

Sezin Anıl
Türkiye

Gözde Hasbal This is me
Türkiye

Nurten Ozsoy
Türkiye

Publication Date

June 27, 2025

Submission Date

October 23, 2018

Acceptance Date

March 14, 2019

Published in Issue

Year 2019 Volume: 23 Number: 4

IZ

https://izlik.org/JA67DJ74PY

Cite

RIS / Bibtex

APA

Duranay, S., Anıl, S., Hasbal, G., & Ozsoy, N. (2025). Purification of acethylcholinesterase from the mollusc Mytilus galloprovincialis Lam. and investigation of its kinetic properties. Journal of Research in Pharmacy, 23(4), 759-769. https://izlik.org/JA67DJ74PY

AMA

1.Duranay S, Anıl S, Hasbal G, Ozsoy N. Purification of acethylcholinesterase from the mollusc Mytilus galloprovincialis Lam. and investigation of its kinetic properties. J. Res. Pharm. 2025;23(4):759-769. https://izlik.org/JA67DJ74PY

Chicago

Duranay, Servet, Sezin Anıl, Gözde Hasbal, and Nurten Ozsoy. 2025. “Purification of Acethylcholinesterase from the Mollusc Mytilus Galloprovincialis Lam. And Investigation of Its Kinetic Properties”. Journal of Research in Pharmacy 23 (4): 759-69. https://izlik.org/JA67DJ74PY.

EndNote

Duranay S, Anıl S, Hasbal G, Ozsoy N (June 1, 2025) Purification of acethylcholinesterase from the mollusc Mytilus galloprovincialis Lam. and investigation of its kinetic properties. Journal of Research in Pharmacy 23 4 759–769.

IEEE

[1]S. Duranay, S. Anıl, G. Hasbal, and N. Ozsoy, “Purification of acethylcholinesterase from the mollusc Mytilus galloprovincialis Lam. and investigation of its kinetic properties”, J. Res. Pharm., vol. 23, no. 4, pp. 759–769, June 2025, [Online]. Available: https://izlik.org/JA67DJ74PY

ISNAD

Duranay, Servet - Anıl, Sezin - Hasbal, Gözde - Ozsoy, Nurten. “Purification of Acethylcholinesterase from the Mollusc Mytilus Galloprovincialis Lam. And Investigation of Its Kinetic Properties”. Journal of Research in Pharmacy 23/4 (June 1, 2025): 759-769. https://izlik.org/JA67DJ74PY.

JAMA

1.Duranay S, Anıl S, Hasbal G, Ozsoy N. Purification of acethylcholinesterase from the mollusc Mytilus galloprovincialis Lam. and investigation of its kinetic properties. J. Res. Pharm. 2025;23:759–769.

MLA

Duranay, Servet, et al. “Purification of Acethylcholinesterase from the Mollusc Mytilus Galloprovincialis Lam. And Investigation of Its Kinetic Properties”. Journal of Research in Pharmacy, vol. 23, no. 4, June 2025, pp. 759-6, https://izlik.org/JA67DJ74PY.

Vancouver

1.Servet Duranay, Sezin Anıl, Gözde Hasbal, Nurten Ozsoy. Purification of acethylcholinesterase from the mollusc Mytilus galloprovincialis Lam. and investigation of its kinetic properties. J. Res. Pharm. [Internet]. 2025 Jun. 1;23(4):759-6. Available from: https://izlik.org/JA67DJ74PY