Screening of some Sumatran medicinal plants and selected secondary metabolites for their cytotoxic potential against MCF-7 and HSC-3 cell lines

Husnunnisa Husnunnisa; Friardi Ismed; Muhammad Taher; Solachuddin J.a. Ichwan; Amri Bakhtiar; Dayar Arbain

Screening of some Sumatran medicinal plants and selected secondary metabolites for their cytotoxic potential against MCF-7 and HSC-3 cell lines

Abstract

In continuation of our study of Sumatran plants and bioactivities of their constituents particularly their cytotoxic activities, we re-extracted lichen Stereocaulon graminosum Schaer (Stereocaulaceae), and isolated atranorin (1) and stictic acid (4).. These isolated compounds have been tested for their cytotoxic activities against human breast adenocarcinoma cancer cell (MCF-7) and human oral squamous carcinoma cancer cell (HSC-3) using MTT assay with a slight modification. Some methanolic extracts of the leaves of Elephantopus scaber, Piper betle, Andrographis paniculata, rhizomes of Boesenbergia pandurata, Alpinia galanga, Kaempferia galanga and isolated compounds; ethyl haematommate (2), olivatolic acid (3), deoxyelephantopin (5), andrographolide (6), hydroxychavicol (7), eugenol (8), curcumin (9), pinostrobin (10), pinocembrin (11), alpinetin (12), α-mangostin (13), angiopteroside (14), and stevioside (15) from Sumatran traditional medicinal plants have also been tested.

Keywords

References

[1] Wahidin M, Noviani R, Hermawan S, Andriani V, Ardian A, Djarir H. Population-based cancer registration in Indonesia. Asian Pac J Cancer Prev. 2012; 13: 1709-1710. [CrossRef]
[2] Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D, Bray F. Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer Suppl. 2015; 136: E359–E386. [CrossRef]
[3] Ng CH, Phaty NB, Taib NA, Teh YC, Mun KS, Amiruddin A, Evlina S, Rhodes A, Yip CH. Comparison of breast cancer in Indonesia and Malaysia – A Clinico-pathological study between Dharmais Cancer Centre Jakarta and University Malaya Medical Centre, Kuala Lumpur. Asian Pac J Cancer Prev. 2011; 12: 2943-2946.
[4] Sutandyo N, Ramli R, Sari L, Soeis DS. Profile and survival of tongue cancer patients in “Dharmais” Cancer Hospital, Jakarta. Asian Pac J Cancer Prev. 2014; 15(5): 1971-1975. [CrossRef]
[5] Elfahmi, Woerdenbag HJ, Kayser O. Jamu: Indonesian traditional herbal medicine towards rational phytopharmacological use. J Herbal Med. 2014; 69: 1-23. [CrossRef]
[6] Arbain D. Inventory, constituents and conservation of biologically important Sumatran plants. Nat Prod Commun. 2012; 7(6): 799-806.
[7] Ismed F, Bakhtiar A. 2017. Production of Ellagic Acid, Panduratin, and 20-hydroxyecdyson as Marker of Indonesian Traditional Medicinal Plants, Research Report, Andalas University, Department of Health Republic of Indonesia.
[8] Ismed F, Arifa N, Zaini E, Bakhtiar A, Umeda D, Putra OD, Yonemochi. Ethyl Haematommate from Stereocaulon graminosum Schaer: Isolation and crystal structure. Nat Prod Sci. 2018; 24(2): 115-118. [CrossRef]

[9] Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxic assays. J Immunol Methods. 1983; 65(1-2): 55-63.
[10] Al-Zikri PNH, Taher M, Susanti D, Ichwan SJA. Cytotoxic activity of Luvunga scandens against human cancer cell lines. Jurnal Teknologi (Sciences & Engineering). 2016; 78(10): 153-157. [CrossRef]
[11] Ismed F, Devehat FLL, Rouaud I, Ferron S, Bakhtiar A, Boustie J. NMR reassignment of stictic acid isolated from Sumatran lichen Stereocaulon montagneanum (Stereocaulaceae) with superoxide anion scavenging activities. Z Naturforsch C. 2017; 72(1-2): 55-62. [CrossRef]
[12] Malhotra S, Subban R, Singh A. Lichens – role in traditional medicine and drug discovery. Internet J Altern Med. 2007; 5(2): 1-6.
[13] Pejin B, Iodice C, Bogdanovic G, Kojic V, Tesevic V. Stictic acid inhibits cell growth of human colon adenocarcinoma HT-29 cells. Arab J Chem. 2017; 10: S1240-S1242. [CrossRef]
[14] Chahyadi A, Hartati R, Wirasutisna KR, Elfahmi. Boesenbergia pandurata Roxb., an Indonesian medicinal plant: Phytochemistry, biological activity, plant biotechnology. Procedia Chem. 2014; 13: 13-37. [CrossRef]
[15] Ashidi JS, Houghton PJ, Hylands PJ, Efferth T. Ethnobotanical survey and cytotoxicity testing of plants of southwestern Nigeria used to treat cancer, with isolation of cytotoxic constituents from Cajanus cajan Millsp. leaves. J Ethnopharmacol. 2010; 128: 501-512. [CrossRef]
[16] Poerwono H, Sasaki S, Hattori Y, Higashiyama K. Efficient microwave-assisted prenylation of pinostrobin and biological evaluation of its derivatives as antitumor agents. Bioorg Med Chem Lett. 2010; 20: 2086-2089. [CrossRef]
[17] Umehara K, Nemoto K, Kimijima K, Matsushita A, Terada E, Monthakantirat O, Eknamkul WD, Miyase T, Warashina T, Degawa M, Noguchi H. Estrogenic constituents of the heartwood of Dalbergia parviflora. Phytochemistry. 2008; 69: 546-552. [CrossRef]
[18] Hatcher H, Planalp R, Cho J, Torti FM, Torti SV. Curcumin: From ancient medicine to current clinical trials. Cell Mol Life Sci. 2008; 65: 1631-1652. [CrossRef]
[19] Umadevi M, Kumar KPS, Bhowmik D, Duraivel S. Traditionally used anticancer herbs in India. J Med Plant Stud. 2013; 1(3): 56-74.
[20] Mohamed GA, Al-Abd AM, El-halawany AM, Abdallah HM, Ibrahim SRM. New xanthones and cytotoxic constituents from Garcinia mangostana fruit hulls against human hepatocellular, breast, and colorectal cancer cell lines. J Ethnopharmacol. 2017; 198: 302-312. [CrossRef]
[21] Putra DP. 2015. Production of ten marker compounds as standard comparison of Indonesian traditional medicinal plants, Research Report, Andalas University – Department of Health, Republic of Indonesia.
[22] Ismed F. 2014. Production of major constituents of ten isolation medicinal plants for marker of Indonesian Herbal Pharmacopeia, Research Report, Andalas University, Department of Health Republic of Indonesia.
[23] Putra DP. 2014. Development and production of standardized terpenoid and isolate compound of iso/deoxyelephantopin from Indonesian traditional medicinal plants Elephantopus scaber (L), Research Report, Andalas University – Department of Health, Republic of Indonesia.
[24] Anggia V, Bakhtiar A, Arbain D. Chemical constituents and antibacterial activities of leaves of Sumatran King Fern (Angiopteris evecta G. Forst HOFFM.). Jurnal Farmasi Indonesia. 2015; 7(4): 195-202.
[25] Ismed F, Farhan A, Bakhtiar A, Zaini E, Nugraha YP, Putra OD, Uekusa H. Crystal structure of olivetolic acid: a natural product from Cetrelia sanguinea (Schaer.). Acta Crystallogr E Crystallogr Commun. 2016; 72(11): 1587-1589. [CrossRef]
[26] Arbain D, Putra DP. 2016. Simplicia and extract of Stevia Herb (Stevia rebaudiana Bert.), Research Report, Andalas University, Department of Health Republic of Indonesia.
[27] Ismed F, Devehat FLL, Delalande O, Sinbandhit S, Bakhtiar A, Boustie J. Lobarin from the Sumatran lichen, Stereocaulon halei. Fitoterapia. 2012; 83: 1693-1698.

Details

Primary Language

English

Subjects

Pharmaceutical Microbiology

Journal Section

Research Article

Authors

Husnunnisa Husnunnisa This is me
Indonesia

Friardi Ismed This is me
Malaysia

Muhammad Taher
Malaysia

Solachuddin J.a. Ichwan
Malaysia

Amri Bakhtiar This is me
Indonesia

Dayar Arbain ^* This is me
Indonesia

Publication Date

June 27, 2025

Submission Date

October 3, 2018

Acceptance Date

February 24, 2019

Published in Issue

Year 2019 Volume: 23 Number: 4

IZ

https://izlik.org/JA85ET67LW

Cite

RIS / Bibtex

APA

Husnunnisa, H., Ismed, F., Taher, M., Ichwan, S. J., Bakhtiar, A., & Arbain, D. (2025). Screening of some Sumatran medicinal plants and selected secondary metabolites for their cytotoxic potential against MCF-7 and HSC-3 cell lines. Journal of Research in Pharmacy, 23(4), 770-776. https://izlik.org/JA85ET67LW

AMA

1.Husnunnisa H, Ismed F, Taher M, Ichwan SJ, Bakhtiar A, Arbain D. Screening of some Sumatran medicinal plants and selected secondary metabolites for their cytotoxic potential against MCF-7 and HSC-3 cell lines. J. Res. Pharm. 2025;23(4):770-776. https://izlik.org/JA85ET67LW

Chicago

Husnunnisa, Husnunnisa, Friardi Ismed, Muhammad Taher, Solachuddin J.a. Ichwan, Amri Bakhtiar, and Dayar Arbain. 2025. “Screening of Some Sumatran Medicinal Plants and Selected Secondary Metabolites for Their Cytotoxic Potential Against MCF-7 and HSC-3 Cell Lines”. Journal of Research in Pharmacy 23 (4): 770-76. https://izlik.org/JA85ET67LW.

EndNote

Husnunnisa H, Ismed F, Taher M, Ichwan SJ, Bakhtiar A, Arbain D (June 1, 2025) Screening of some Sumatran medicinal plants and selected secondary metabolites for their cytotoxic potential against MCF-7 and HSC-3 cell lines. Journal of Research in Pharmacy 23 4 770–776.

IEEE

[1]H. Husnunnisa, F. Ismed, M. Taher, S. J. Ichwan, A. Bakhtiar, and D. Arbain, “Screening of some Sumatran medicinal plants and selected secondary metabolites for their cytotoxic potential against MCF-7 and HSC-3 cell lines”, J. Res. Pharm., vol. 23, no. 4, pp. 770–776, June 2025, [Online]. Available: https://izlik.org/JA85ET67LW

ISNAD

Husnunnisa, Husnunnisa - Ismed, Friardi - Taher, Muhammad - Ichwan, Solachuddin J.a. - Bakhtiar, Amri - Arbain, Dayar. “Screening of Some Sumatran Medicinal Plants and Selected Secondary Metabolites for Their Cytotoxic Potential Against MCF-7 and HSC-3 Cell Lines”. Journal of Research in Pharmacy 23/4 (June 1, 2025): 770-776. https://izlik.org/JA85ET67LW.

JAMA

1.Husnunnisa H, Ismed F, Taher M, Ichwan SJ, Bakhtiar A, Arbain D. Screening of some Sumatran medicinal plants and selected secondary metabolites for their cytotoxic potential against MCF-7 and HSC-3 cell lines. J. Res. Pharm. 2025;23:770–776.

MLA

Husnunnisa, Husnunnisa, et al. “Screening of Some Sumatran Medicinal Plants and Selected Secondary Metabolites for Their Cytotoxic Potential Against MCF-7 and HSC-3 Cell Lines”. Journal of Research in Pharmacy, vol. 23, no. 4, June 2025, pp. 770-6, https://izlik.org/JA85ET67LW.

Vancouver

1.Husnunnisa Husnunnisa, Friardi Ismed, Muhammad Taher, Solachuddin J.a. Ichwan, Amri Bakhtiar, Dayar Arbain. Screening of some Sumatran medicinal plants and selected secondary metabolites for their cytotoxic potential against MCF-7 and HSC-3 cell lines. J. Res. Pharm. [Internet]. 2025 Jun. 1;23(4):770-6. Available from: https://izlik.org/JA85ET67LW