Antioxidant and acetylcholinesterase inhibitor activity of polar extracts obtained from Thymus cilicicus

Nur Tanır Özkanlı; Selin Tufan; Turgut Taşkın; Nur Tan

doi:10.12991/jrespharm.1748360

Antioxidant and acetylcholinesterase inhibitor activity of polar extracts obtained from Thymus cilicicus

Abstract

The Lamiaceae family comprises a large number of aromatic plant species with notable medicinal, culinary, and cosmetic applications. Within this family, the genus Thymus widely used in Turkish folk medicine. Thymus cilicicus, has been also used in traditional practices in the form of herbal teas, aromatic waters, and essential oils for the treatment of ailments such as toothache, stomach discomfort, respiratory disorders, diabetes, hypertension, and the common cold. Previous studies have reported antioxidant, antimicrobial, antidiabetic, cytotoxic, and acetylcholinesterase (AChE) inhibitory properties in T. cilicicus extracts obtained with organic solvents. These activities are mainly associated with the presence of phenolic compounds. However, existing literature has not yet addressed the biological activity and chemical composition of extracts obtained through traditional preparation methods such as infusion or maceration. Present study investigates antioxidant capacity such as 1,1-diphenyl-2-picrylhydrazyl radical scavenging (DPPH), ferric reducing antioxidant potency (FRAP) and cupric reducing antioxidant capacity (CUPRAC) along with AChE inhibitory activities of T. cilicicus extracts (ethanol maceration, water maceration, and infusion). Chemical profiles of the extracts were assessed via total phenolic content determination and LC-HRMS. LC-HRMS profiling of tested extracts revealed that major compounds of extracts were fumaric acid, p-coumaric acid, sinapinic acid, and, caffeic acid. Ethanol macerate extract exhibited higher total phenolic content and antioxidant activity in DPPH radical scavenging and CUPRAC assays compared to traditional preparation forms, while infusion extract exhibited highest activity in FRAP assay. None of the tested extract showed AChE inhibition at 100 µg/mL.

Keywords

Project Number

Scientific Research Projects Coordination Unit of Istanbul University. Project number TLO-2019-35055.

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Details

Primary Language

English

Subjects

Pharmacognosy

Journal Section

Research Article

Authors

Nur Tanır Özkanlı
0000-0002-7761-6546
Türkiye

Selin Tufan ^*
0000-0002-9173-3423
Türkiye

Turgut Taşkın
0000-0001-8475-6478
Türkiye

Nur Tan
0000-0001-7958-1917
Türkiye

Publication Date

May 11, 2026

Submission Date

July 23, 2025

Acceptance Date

February 9, 2026

Published in Issue

Year 2026 Volume: 30 Number: 3

DOI

https://doi.org/10.12991/jrespharm.1748360

IZ

https://izlik.org/JA56WY72KS

Cite

RIS / Bibtex

APA

Tanır Özkanlı, N., Tufan, S., Taşkın, T., & Tan, N. (2026). Antioxidant and acetylcholinesterase inhibitor activity of polar extracts obtained from Thymus cilicicus. Journal of Research in Pharmacy, 30(3), 933-944. https://doi.org/10.12991/jrespharm.1748360

AMA

1.Tanır Özkanlı N, Tufan S, Taşkın T, Tan N. Antioxidant and acetylcholinesterase inhibitor activity of polar extracts obtained from Thymus cilicicus. J. Res. Pharm. 2026;30(3):933-944. doi:10.12991/jrespharm.1748360

Chicago

Tanır Özkanlı, Nur, Selin Tufan, Turgut Taşkın, and Nur Tan. 2026. “Antioxidant and Acetylcholinesterase Inhibitor Activity of Polar Extracts Obtained from Thymus Cilicicus”. Journal of Research in Pharmacy 30 (3): 933-44. https://doi.org/10.12991/jrespharm.1748360.

EndNote

Tanır Özkanlı N, Tufan S, Taşkın T, Tan N (May 1, 2026) Antioxidant and acetylcholinesterase inhibitor activity of polar extracts obtained from Thymus cilicicus. Journal of Research in Pharmacy 30 3 933–944.

IEEE

[1]N. Tanır Özkanlı, S. Tufan, T. Taşkın, and N. Tan, “Antioxidant and acetylcholinesterase inhibitor activity of polar extracts obtained from Thymus cilicicus”, J. Res. Pharm., vol. 30, no. 3, pp. 933–944, May 2026, doi: 10.12991/jrespharm.1748360.

ISNAD

Tanır Özkanlı, Nur - Tufan, Selin - Taşkın, Turgut - Tan, Nur. “Antioxidant and Acetylcholinesterase Inhibitor Activity of Polar Extracts Obtained from Thymus Cilicicus”. Journal of Research in Pharmacy 30/3 (May 1, 2026): 933-944. https://doi.org/10.12991/jrespharm.1748360.

JAMA

1.Tanır Özkanlı N, Tufan S, Taşkın T, Tan N. Antioxidant and acetylcholinesterase inhibitor activity of polar extracts obtained from Thymus cilicicus. J. Res. Pharm. 2026;30:933–944.

MLA

Tanır Özkanlı, Nur, et al. “Antioxidant and Acetylcholinesterase Inhibitor Activity of Polar Extracts Obtained from Thymus Cilicicus”. Journal of Research in Pharmacy, vol. 30, no. 3, May 2026, pp. 933-44, doi:10.12991/jrespharm.1748360.

Vancouver

1.Nur Tanır Özkanlı, Selin Tufan, Turgut Taşkın, Nur Tan. Antioxidant and acetylcholinesterase inhibitor activity of polar extracts obtained from Thymus cilicicus. J. Res. Pharm. 2026 May 1;30(3):933-44. doi:10.12991/jrespharm.1748360