Deciphering the anti-inflammatory pathways: Juniperus macrocarpa's role in IL-6 and TNF modulation in gastric ulcer healing on rats model

Sura Ahmed Al Jabbara; Mohammed Qasim Yahya Malallah A. Al-atrakji

Deciphering the anti-inflammatory pathways: Juniperus macrocarpa's role in IL-6 and TNF modulation in gastric ulcer healing on rats model

Abstract

Juniperus macrocarpa is a native North American juniper species that have a wide range of biological actions such as analgesic, anti-inflammatory, antibacterial, antioxidant, hepatoprotective, and hypoglycemic effect. Therefore, the present study aimed to evaluate the gastropreventive and curable effect of J. macrocarpa extract against indomethacin-induced gastric ulcer in forty-eight healthy male albino rats, aged 6-12 months and weighing 160-250 g that divided into groups; control group and rats subjected to peptic ulcer induction using indomethacin in a prospective, controlled, comparable experimental animal study. Induced groups were treated with omeprazole as a reference drug, pretreated with J. macrocarpa extract, or treated post-induction with varying doses of the extract over 14 days with assessment of interleukin-6 (IL-6), tumor necrosis factor (TNF) levels and histopathological features of gastric mucosa which revealed that the group treated with Indomethacin alone exhibited the highest mean levels of both TNF and IL-6, indicating a pronounced inflammatory response. Conversely, groups receiving J. macrocarpa treatments showed moderated inflammatory markers, underscoring the plant's potential anti-inflammatory effects. The strong positive correlation between TNF and IL-6 further confirms the interconnectedness of these inflammatory biomarkers and these results supported by the histological findings which revealed a significant improvement in the gastric tissue in rats received the extract either as a treatment or as protective remedy which conclusively demonstrates the significant therapeutic potential of J. macrocarpa in treating gastric ulcers by reducing inflammation with a histopathological improvement which indicate that J. macrocarpa can be considered as a promising natural adjunct in gastric ulcer management.

Keywords

References

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Details

Primary Language

English

Subjects

Basic Pharmacology

Journal Section

Research Article

Authors

Sura Ahmed Al Jabbara ^* This is me
0009-0000-1889-2332
Iraq

Mohammed Qasim Yahya Malallah A. Al-atrakji This is me
0000-0001-5047-5854
Iraq

Publication Date

September 1, 2025

Submission Date

September 18, 2024

Acceptance Date

December 21, 2024

Published in Issue

Year 2025 Volume: 29 Number: 5

IZ

https://izlik.org/JA77YU39AX

Cite

RIS / Bibtex

APA

Al Jabbara, S. A., & A. Al-atrakji, M. Q. Y. M. (2025). Deciphering the anti-inflammatory pathways: Juniperus macrocarpa’s role in IL-6 and TNF modulation in gastric ulcer healing on rats model. Journal of Research in Pharmacy, 29(5), 1904-1917. https://izlik.org/JA77YU39AX

AMA

1.Al Jabbara SA, A. Al-atrakji MQYM. Deciphering the anti-inflammatory pathways: Juniperus macrocarpa’s role in IL-6 and TNF modulation in gastric ulcer healing on rats model. J. Res. Pharm. 2025;29(5):1904-1917. https://izlik.org/JA77YU39AX

Chicago

Al Jabbara, Sura Ahmed, and Mohammed Qasim Yahya Malallah A. Al-atrakji. 2025. “Deciphering the Anti-Inflammatory Pathways: Juniperus Macrocarpa’s Role in IL-6 and TNF Modulation in Gastric Ulcer Healing on Rats Model”. Journal of Research in Pharmacy 29 (5): 1904-17. https://izlik.org/JA77YU39AX.

EndNote

Al Jabbara SA, A. Al-atrakji MQYM (September 1, 2025) Deciphering the anti-inflammatory pathways: Juniperus macrocarpa’s role in IL-6 and TNF modulation in gastric ulcer healing on rats model. Journal of Research in Pharmacy 29 5 1904–1917.

IEEE

[1]S. A. Al Jabbara and M. Q. Y. M. A. Al-atrakji, “Deciphering the anti-inflammatory pathways: Juniperus macrocarpa’s role in IL-6 and TNF modulation in gastric ulcer healing on rats model”, J. Res. Pharm., vol. 29, no. 5, pp. 1904–1917, Sept. 2025, [Online]. Available: https://izlik.org/JA77YU39AX

ISNAD

Al Jabbara, Sura Ahmed - A. Al-atrakji, Mohammed Qasim Yahya Malallah. “Deciphering the Anti-Inflammatory Pathways: Juniperus Macrocarpa’s Role in IL-6 and TNF Modulation in Gastric Ulcer Healing on Rats Model”. Journal of Research in Pharmacy 29/5 (September 1, 2025): 1904-1917. https://izlik.org/JA77YU39AX.

JAMA

1.Al Jabbara SA, A. Al-atrakji MQYM. Deciphering the anti-inflammatory pathways: Juniperus macrocarpa’s role in IL-6 and TNF modulation in gastric ulcer healing on rats model. J. Res. Pharm. 2025;29:1904–1917.

MLA

Al Jabbara, Sura Ahmed, and Mohammed Qasim Yahya Malallah A. Al-atrakji. “Deciphering the Anti-Inflammatory Pathways: Juniperus Macrocarpa’s Role in IL-6 and TNF Modulation in Gastric Ulcer Healing on Rats Model”. Journal of Research in Pharmacy, vol. 29, no. 5, Sept. 2025, pp. 1904-17, https://izlik.org/JA77YU39AX.

Vancouver

1.Sura Ahmed Al Jabbara, Mohammed Qasim Yahya Malallah A. Al-atrakji. Deciphering the anti-inflammatory pathways: Juniperus macrocarpa’s role in IL-6 and TNF modulation in gastric ulcer healing on rats model. J. Res. Pharm. [Internet]. 2025 Sep. 1;29(5):1904-17. Available from: https://izlik.org/JA77YU39AX