Determination and data analysis for Pb, Ni, Fe, Mn and Co elements in serum of PCOS patients

Year 2025, Volume: 29 Issue: 1, 75 - 80

Salam A.h. Al-ameri Amer Hasan Abdullah Muntadher H. Challoob

Abstract

Number of women with polycystic ovary syndrome (PCOS) who have an insulin resistance at the same time was increased to large numbers in Iraq, with a lack of statistics regarding the number of PCOS patients and the role of some of biochemical variables that may interfere and causing this disease. The research aimed to study the levels of some trace elements namely; lead, nickel, iron, manganese and cobalt in the serum of Iraqi PCOS patient in a case control study in which the serum Pb, Ni, Fe, Mn and Co concentrations were determined in sixty women with PCOS with an age ranged from 20-40 years old and compared with those levels in 60 age, gender, and BMI-matched healthy volunteers selected as a control group which showed that the levels of Lead and nickel were significantly higher (p<0.05) in the levels of Fe, Mn and Co in patients comparing with controls. The analysis of the ROC curve analysis revealed that all studied trace elements provide significant results with highest values of AUC, sensitivity and specificity obtained with Ni which is 0.954, 85% and 98.3% respectively and in Mn of 0.908, 86.7 % and 88.3 % respectively which indicate that these elements can be considered as good predictive markers for PCOS

Keywords

PCOS, Lead, Nickel, Manganese, Iron, Cobalt

References

• [1] Yaman M, Kaya G, Yekeler H. Distribution of trace metal concentrations in paired cancerous and non-cancerous human stomach tissues. World J Gastroenterol. 2007;13(4):612-618. https://doi.org/10.3748%2Fwjg.v13.i4.612
• [2] Tapiero H, Tew KD. Trace elements in human physiology and pathology: Zinc and metallothioneins. Biomed Pharmacother. 2003;57(9):399-411. https://doi.org/10.1016/s0753-3322(03)00081-7
• [3] Kurdoglu Z, Kurdoglu M, Demir H, Sahin HG. Serum trace elements and heavy metals in polycystic ovary syndrome. Hum Exp Toxicol. 2012;31(5):452-456. https://doi.org/10.1177/0960327111424299
• [4] Pokorska-Niewiada K, Brodowska A, Szczuko M. The content of minerals in the PCOS group and the correlation with the parameters of metabolism. Nutrients. 2021;13(7):2214. https://doi.org/10.3390/nu13072214
• [5] Ozden O, Park SH, Kim HS, Jiang H, Coleman MC, Spitz DR, Gius D. Acetylation of MnSOD directs enzymatic activity responding to cellular nutrient status or oxidative stress. Aging (Albany NY). 2011;3(2):102-107. https://doi.org/10.18632%2Faging.100291
• [6] Saker F, Ybarra J, Leahy P, Hanson RW, Kalhan SC, Ismail-Beigi F. Glycemia-lowering effect of cobalt chloride in the Role of decreased gluconeogenesis. Am J Physiol. 1998;274(6):E984-991. https://doi.org/10.1152/ajpendo.1998.274.6.e984
• [7] Jomova K, Valko M. Advances in metal-induced oxidative stress and human disease. Toxicology. 2011;283(2-3):65 87. https://doi.org/10.1016/j.tox.2011.03.001
• [8] Ćwiertnia A, Kozłowski M, Cymbaluk-Płoska A. The role of ıron and cobalt in gynecological diseases. Cells. 2022;12(1):117. https://doi.org/10.3390%2Fcells12010117
• [9] Abudawood M, Tabassum H, Alanazi AH, Almusallam F, Aljaser F, Ali MN, Alenzi ND, Alanazi ST, Alghamdi MA, Altoum GH, Alzeer MA, Alotaibi MO, Abudawood A, Ghneim HK, Al-Nuaim LAA. Antioxidant status in relation to heavy metals induced oxidative stress in patients with polycystic ovarian syndrome (PCOS). Sci Rep. 2021;11(1):22935. https://doi.org/10.1038/s41598-021-02120-6
• [10] Kuşçu NK, Var A. Oxidative stress but not endothelial dysfunction exists in non-obese, young group of patients with polycystic ovary syndrome. Acta Obstet Gynecol Scand. 2009;88(5):612-617. https://doi.org/10.1080/00016340902859315
• [11] Luque-Ramírez M, Alvarez-Blasco F, Botella-Carretero JI, Sanchón R, San Millán JL, Escobar-Morreale HF. Increased body iron stores of obese women with polycystic ovary syndrome are a consequence of insulin resistance and hyperinsulinism and are not a result of reduced menstrual losses. Diabetes Care. 2007;30(9):2309-2313. https://doi.org/10.2337/dc07-0642
• [12] Escobar-Morreale HF. Iron metabolism and the polycystic ovary syndrome. Trends Endocrinol Metab. 2012;23(10):509-515. https://doi.org/10.1016/j.tem.2012.04.003
• [13] Al-Hakeim HK. Correlation Between Iron Status Parameters and Hormone Levels in Women with Polycystic Ovary Syndrome. Clin Med Insights Women's Health. 2012; 5. http://dx.doi.org/10.4137/CMWH.S8780
• [14] Kirmizi DA, Baser E, Turksoy VA, Kara M, Yalvac ES, Gocmen AY. Are heavy metal exposure and trace element levels related to metabolic and endocrine problems in polycystic ovary syndrome? Biol Trace Elem Res. 2020;198(1):77-86. https://doi.org/10.1007/s12011-020-02220-w
• [15] Liang C, Zhang Z, Cao Y, Wang J, Shen L, Jiang T, Li D, Zou W, Zong K, Liang D, Xu X, Liu Y, Tao F, Luo G, Ji D, Cao Y. Exposure to multiple toxic metals and polycystic ovary syndrome risk: Endocrine disrupting effect from As, Pb and Ba. Sci Total Environ. 2022;849:157780. https://doi.org/10.1016/j.scitotenv.2022.157780
• [16] Balahoroglu R, Zirek AK, Cokluk E, Atmaca M, Sekeroglu MZ, Huyut Z, The relationship between insulin resistance and trace elements in patients with polycystic ovary syndrome. Online Turk J Health Sci. 2020; 5(2):375 382. https://doi.org/10.26453/otjhs.571510
• [17] Pokorska-Niewiada K, Brodowska A, Brodowski J, Szczuko M. Levels of trace elements in erythrocytes as endocrine disruptors in obese and nonobese women with polycystic ovary syndrome. Int J Environ Res Public Health. 2022;19(2):976. https://doi.org/10.3390/ijerph19020976
• [18] Taher MA, Mhaibes SH. Assessment of some trace elements in obese and non-obese polycystic ovary syndrome (PCOS). Int J Sci Res. 2017;6(9): 1333-1341.
• [19] Prodarchuk MG, Tatarchuk TF, Gunkov SV, Zhminko PG, Regeda SI, Rymarchuk MI. The role of macro- and microelements in the pathogenesis of polycystosis of the ovaries. Reprod Endocrinol. 2020; 53: 19–22. http://dx.doi.org/10.18370/2309-4117.2019.48.22-25
• [20] Sharma P, Gupta V, Kumar K, Khetarpal P. Assessment of serum elements concentration and polycystic ovary syndrome (PCOS): Systematic review and meta-analysis. Biol Trace Elem Res. 2022;200(11):4582-4593. https://doi.org/10.1007/s12011-021-03058-6
• [21] Yin J, Hong X, Ma J, Bu Y, Liu R. Serum trace elements in patients with polycystic ovary syndrome: A systematic review and meta-analysis. Front Endocrinol (Lausanne). 2020;11:572384. https://doi.org/10.3389%2Ffendo.2020.572384
• [22] Li M, Tang Y, Lin C, Huang Q, Lei D, Hu Y. Serum macroelement and microelement concentrations in patients with polycystic ovary syndrome: A cross-sectional study. Biol Trace Elem Res. 2017;176(1):73-80. https://doi.org/10.1007/s12011-016-0782-4
• [23] Cao R, López-de-Ullibarri I. ROC curves for the statistical analysis of microarray data. Methods Mol Biol. 2019;1986:245-253. https://doi.org/10.1007/978-1-4939-9442-7_11
• [24] Pérez-Fernández S, Martínez-Camblor P, Filzmoser P, Corral N. Visualizing the decision rules behind the ROC curves: understanding the classification process. Adv Stat Anal. 2021;105:135–161. http://dx.doi.org/10.1007/s10182-020-00385-2.
• [25] Aldafaay AAA, Abdulamir HA, Abdulhussain HA, Badry AS, Abdulsada AK. The use of urinary α-amylase level in a diagnosis of chronic renal failure. Res J Pharm Technol. 2021; 14(3):1597-1600. http://dx.doi.org/10.5958/0974 360X.2021.00283.3