Effect of different additives on amylase activity

Year 2025, Volume: 29 Issue: 1, 346 - 351

Safija Herenda , Selma Fetahović Nataša đorđević Tamara Klisara Edhem Hasković Sabina Prevljak

Abstract

Enzymes are catalysts of biological origin, and according to their chemical composition, they are simple or
complex proteins. There are several theories about the enzyme's mechanism of action. Today, the Michaelis-Menten
theory is generally accepted. According to this theory, during enzymatic reactions, an intermediate compound is created
between the enzyme and the substrate. After the formation of this complex, the enzyme catalyzes a chemical reaction
that changes the substrate into another molecule, which we call the product. The product is then separated and released
from the active site of the enzyme, which is then ready to bind the next substrate molecule. Enzyme activity can be
affected by different molecules. The purpose of this study is to use the spectrophotometric approach to determine
whether sodium benzoate and ascorbic acid (vitamin C) serve as activators or inhibitors of enzymatic reactions. The
obtained results show that both additives bind to the enzyme-substrate complex, causing non-competitive inhibition.

Keywords

Enzymes, additives, ascorbic acid, sodium benzoate, inhibition

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