Formulation, characterization and antidepressant evaluation of phyto-assisted selenium nanoparticles synthesized using Tinospora cordifolia aqueous extract

Year 2025, Volume: 29 Issue: 1, 352 - 359, 03.03.2025

Radhika Chaurasia Surya Prakash Gupta

https://doi.org/10.12991/jrespharm.1644439

Abstract

Tinospora cordifolia extracts are extensively used in various herbal preparations for the treatment of
different ailments for its anti-periodic, anti-spasmodic, anti-microbial, anti-osteoporotic, anti-inflammatory, anti-arthritic,
anti-allergic, and anti-diabetic properties. In the present investigation the aqueous extract of leaf of Tinospora cordifolia
was used as reducing agent for the synthesis of Selenium nanoparticles. The total phenolic content of the extract was
determined by derivatization with Folin-ciocalteu reagent and measuring the absorbance in UV-visible
spectrophotometer at 765 nm. The SeNPs prepared by reduction of sodium selenite by the extract were assessed in terms
of FTIR, UV absorption, size, and form and antidepressant action using forced swim test in mice. The extract solution
was dark green in color and contained 1.3 ± 0.003 GAE/mg of phenolics. Se NP was produced rapidly with ascorbic acid
as well as Tinospora cordifolia extract. The formation of Se NP is indicated by an absorbance at 226.0 nm in the ultraviolet
spectrum. The FTIR spectrum revealed the stretching and bending vibrations of O-H, C-H, C-C, N-O, C-N, and other
groups due to the presence of phytoconstituent composition. It was discovered that the concentration of the extract, or
reducing agent, had an impact on the size of the Se NP nanoparticles, which ranged in size from 46 to 137 nm. The SEM
images showed smooth particles with a spherical structure. The Se NPs synthesized using Tinospora cordifolia leaf extract
exhibited antidepressant action in a concentration dependent manner. The lowest immobility time was depicted by Se
NPE4 (1.115 ± 0.0213 min)..

Keywords

Selenium nanoparticles , Tinospora cordifolia , Biogenic reduction , sodium selenite

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