Analysis of anti-aging activity of Chinese perfume (Aglaia odorata) and Indian camphorweed (Pluchea indica) leaves using Saccharomyces cerevisiae model system

Year 2025, Volume: 29 Issue: 1, 396 - 406

Woro Anindito Sri Tunjung

Abstract

This study examined the anti-aging effect of Chinese perfume (Aglaia odorata) and Indian camphorweed
(Pluchea indica) leaf extracts on Saccharomyces cerevisiae as a model system. Investigation of the antioxidant activity and
bioactive compounds using GC–MS and qualitative anti-aging spot tests were performed to determine the anti-aging
effects. In addition, a quantitative anti-aging test was conducted using high-throughput chronological lifespan analysis.
The results showed that the antioxidant enzyme activities of SOD, APX, and CAT in Chinese perfume leaves were 393.96
units/min/g FW, 215 µmoles H2O2/min/g FW, and 5.6 µmoles H2O2 decomposed/min/g FW, respectively; the values
in Indian camphorweed leaves were 717.57 units/min/g FW, 48 µmoles H2O2/min/g FW, and 12.33 µmole H2O2
decomposed/min/g FW, respectively. The antioxidant activity of Chinese perfume and Indian camphorweed was 577.2
µg/mL and 348.86 µg/mL. The antioxidant bioactive compounds of Chinese perfume extract included n-hexadecanoic
acid, β turmerone, and 2-propenoic acid, 3-phenyl-, methyl ester (methyl cinnamate) and those from Indian
camphorweed included n-hexadecanoic acid and neophytadiene. Treatment with both extracts prolonged the life of
yeast after 15 days of incubation. In addition, H2O2 stress conditions, the yeasts showed better growth with the addition
of both leaf extracts. This study revealed that the extracts of Chinese perfume and Indian camphorweed leaves
demonstrate promising potential as ingredients for anti-aging cosmetics.

Keywords

Chinese perfume, Indian camphorweed, anti-aging, antioxidant, S. cerevisiae

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