The effectiveness test of film forming hydrogel (FFH) of Yellow Root Extract (Arcangelisia flava (L.) Merr) on healing burns of male white mice (Mus Musculus)

Year 2025, Volume: 29 Issue: 2, 516 - 529

Rahmi Annisa Irbah Mahdiyah Salsabila Siti Mei Astuti Arief Suryadinata Ria Ramadhani Dwi Atmaja Roihatul Mutiah

https://doi.org/10.12991/jrespharm.1662042

Abstract

The Burns are conditions where skin tissue is lost due to direct contact with heat energy. Therefore, a preparation was developed as film forming hydrogel (FFH) from yellow root extract. Yellow roots contain alkaloids, flavonoids, and saponins, which can improve wound healing. This study aims to determine the effect of the concentration of yellow root extract on wound diameter percentage and increasing the number of fibroblast cells and epithelial thickness. This research uses a true experimental laboratory with a design pre-post test study method on mice aged 2-3 months weighing 20-35 g. Samples were taken using a random sampling technique and divided into five treatment groups, namely formula 1 (F1) with 0.5% yellow root extract, formula 2 (F2) with 1% yellow root extract (F2), formula 3 (F3) with 1.5% yellow root extract, bioplacentonÒ (positive control), normal saline 0.9% (negative control). Treatment of mice for 14 days. The observation parameters were measuring the diameter of the burn wound using the Morton method and the percentage of burn wound healing. The number of fibroblast cells and epithelial thickness were observed at 400x magnification by taking five fields of view in a zig-zag manner. This research shows that the film forming hydrogel (FFH) preparations from yellow root extract with a 1.5% yellow root extract (F3) are the most effective for healing burn wounds. This can be seen from the diameter of the wound, the percentage of wound healing, the increase in fibroblasts, and the thickness of the epithelium.

Keywords

Film Forming Hydrogel (FFH), Arcangelisia flava (L.) Merr, healing burns, fibroblasts, epithelial thickness

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