Aqueous extract of Aloe vera ameliorates ethanol-ınduced liver damage and oxidative stress in Balb/c mice

Year 2025, Volume: 29 Issue: 2, 552 - 560

Jilmari Shadrach Saleh Helga Ishaya , Samaila Musa Chiroma Martha Orendu Oche Attah , Sunday Joseph Manye Nathan Isaac Dibal

https://doi.org/10.12991/jrespharm.1662053

Abstract

Exessive alcohol abuse is associated with physiological and pathological effects on the body. Aloe vera was selected because of its many beneficial qualities. 25 mice were given the following treatment protocol: distilled water (normal control), 20% ethanol at 10ml/kg (negative control), 25mg/kg Aqueous extract of Aloe vera (AA) plus 20% ethanol, 50mg/kg AA plus 20% ethanol and 100mg/kg silymarin plus 20% ethanol (positive control) respectively daily for 18 days. After sacrifice, blood was collected for biochemical analysis. The liver was analysed histologically, histochemically and part was macerated to determine oxidative stress biomarkers. AA significantly decreased (P< .05) AST activity but it did not significantly decrease ALT activity.It significantly increased (P<.05) albumin levels. Pretreatment with AA at 25mg/kg significantly elevated (P< .05) catalase and SOD activities. However, GSH activity was not significantly changed. AA was found to significantly reduce (P< .05) lipid peroxidation. AA significantly increased the number of hepatocytes at telophase stage relative to the ethanol-treated mice. Histological and histochemical analysis revealed normal hepatocytes with few vacuoles and fewer fat droplets in the liver parenchyma of AA treated mice compared to the ethanol group. The results reveal that pretreatment with AA has hepatoprotective effects on ethanolinduced hepatic damage by reducing oxidative stress and preserving the histology of the hepatic tissue.

Keywords

Aloe vera, biomarker, hepatic, histology, oil red o, oxidative stress

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