Antioxidant and antimicrobial activities of Hypericum origanifolium Willd. var. origanifolium

Year 2025, Volume: 29 Issue: 2, 599 - 604

Zeynep Gülcan , Fatmanur Tunç , Nagehan Saltan , Pervin Soyer , Yavuz Bülent Köse

https://doi.org/10.12991/jrespharm.1662102

Abstract

Hypericum origanifolium Willd. var. origanifolium, commonly known as "Lüfer otu". Since the number of studies on this taxon is limited, this study aimed to evaluate its biological activities, especially its antioxidant, and antimicrobial activities. Extracts were prepared with methanol, n-hexane and ethyl acetate solvents from the aerial parts of the plant. In the DPPH· assay, the n-hexane extract exhibited higher antioxidant activity compared to other extracts, with an IC50 value of 0.096±0.021 mg/mL. The highest total phenolic content was observed in the n-hexane extract (75.73±0.016 mg GAE/g extract), while the lowest total phenolic content was found in the ethyl acetate extract (40.42±0.003 mg GAE/g extract). The extracts showed antimicrobial activity against all tested microorganisms at values ranging from 7.81-4000 μg/mL. It was proven to have strong antibacterial activity, especially against S. aureus, B. subtilis, and E. faecalis microorganisms, with its low MIC (minimum inhibitory concentration) value (7.81-62.5 μg/mL). The study's findings show that H. origanifolium var. origanifolium extracts have the potential to be a good source of free radicals. Targeting particular bacterial illnesses may be possible given the notable antibacterial efficacy against particular strains.

Keywords

Antimicrobial, antioxidant, Hypericum origanifolium, phenolic content

References

• [1] Baytop T, Türkiye’de Bitkiler ile Tedavi (Geçmişte ve Bugün), Nobel Tıp Kitabevleri, İstanbul, 1999, pp. 166-167.
• [2] bizimbitkiler.org.tr. https://bizimbitkiler.org.tr/v2/filtre.php?familya=Hypericaceae&taxon=&taxonKriter=0&epitetType=0&epitet=&epitetKriter=0&authorType=0&author=&authorKriter=0&sayfa=1&trkAdi=&trkKriter=0 (accessed March 13, 2024).
• [3] Davis P. (Eds). Flora of Turkey and The East Aegean Islands, Volume 2, Edinburgh Univ, Edinburgh, 1967.
• [4] Yeşilada E, İyileştiren Bitkiler, Hayy kitap, İstanbul, 2019.
• [5] Eatemadnia A, Ansari S, Abedi P, Najar S. The effect of Hypericum perforatum on postmenopausal symptoms and depression: A randomized controlled trial. Complement Ther Med. 2019; 45: 109-113. https://doi.org/10.1016/j.ctim.2019.05.028.
• [6] Saltan N, Köse YB, Kırcı D, Göger F, Demirci B. Chemical profiling and antimicrobial activity of essential oils from Hypericum adenotrichum Spach. An endemic species. J Pharm Res. 2023; 27(4): 1645-1657. http://doi.org/10.29228/jrp.449.
• [7] Medina MA, Martinez-Poveda B, Amores-Sanchez MI, Quesada AR. Hyperforin: More than an antidepressant bioactive compound?. Life Sci. 2006; 79: 105–111. https://doi.org/10.1016/j.lfs.2005.12.027.
• [8] Guedes RC, Eriksson LA. Theoretical study of hypericin. J Photochem Photobiol A. 2005; 172: 293–299. https://doi.org/10.1016/j.jphotochem.2004.12.025.
• [9] Smelcerovic A, Zuehlke S, Spiteller M, Raabe N, Özen T. Phenolic constituents of 17 Hypericum species from Turkey. Biochem Syst Ecol. 2008; 36: 316-319. https://doi.org/10.1016/j.bse.2007.09.002.
• [10] Güzey G, Ibadova S, Öztürk Y, Öztürk N, Maggi F, Sagratini G, Ricciutelli M, Vittori S. Antiproliferative and antioxidant effects of three Hypericum species of Turkish origin: H. perforatum, H. montbretii and H. origanifolium. Med Aromat Plant Sci Biotechnol. 2011; 5(special issue 1): 91-99.
• [11] Bertoli A, Çirak C, Seyis F. Hypericum origanifolium Willd.: The essential oil composition of a new valuable species. Ind Crops Prod. 2015; 77: 676-679. https://doi.org/10.1016/j.indcrop.2015.08.014.
• [12] Kolit D. Mater’s Thesis. Sille (Selçuklu/Konya) ve Çevresinin Etnobotanik Özellikleri. Institute of Science, Gazi University, Ankara, Turkey, 2022.
• [13] Gül LB, Özdemir N, Gül O, Çırak C, Can AH. Some bioactive properties and antimicrobial activity of some Hypericum species growing wild in Black Sea Region of Turkey. Eur Food Sci Eng. 2021; 2(1): 1-6.
• [14] Boran R. Investigations of anti-aging potential of Hypericum origanifolium Willd. for skincare formulations. Ind Crops Prod. 2018; 118: 290-295. https://doi.org/10.1016/j.indcrop.2018.03.058.
• [15] Seyrekoglu F, Temiz H, Eser F, Yildirim C. Comparison of the antioxidant activities and major constituents of three Hypericum species (H. perforatum, H. scabrum and H. origanifolium) from Turkey. S Afr J. 2022; 146: 723-727. https://doi.org/10.1016/j.sajb.2021.12.012.
• [16] Kubin A, Loew HG, Burner U, Jessner G, Kolbabek H, Wierrani F. How to make hypericin water-soluble. Die Pharmazie. 2008; 63: 263–269. https://doi.org/10.1691/ph.2008.7292.
• [17] Kakouri E, Daferera D, Trigas P, Charalambous D, Pantelidou M, Tarantilis PA, Kanakis CD. Comparative study of the antibacterial activity, total phenolic and total flavonoid content of nine Hypericum species grown in Greece. Appl Sci. 2023; 13(5): 3305. https://doi.org/10.3390/app13053305.
• [18] Tocci N, Perenzoni D, Iamonico D, Fava F, Weil T, Mattivi F. Extracts from Hypericum hircinum subsp. majus exert antifungal activity against a panel of sensitive and drug-resistant clinical strains. Front Pharmacol. 2018; 9: 382. https://doi.org/10.3389/fphar.2018.00382.
• [19] Kumarasamy Y, Byres M, Cox PJ, Jaspars M, Nahar, Sarker SD. Screening seeds of some Scottish plants for free radical scavenging activity. Phytother Res. 2007; 21(7): 615-621. https://doi.org/10.1002/ptr.2129.
• [20] Re R, Pellegrini N, Proteggente A, Pannalaa A, Yanga M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorisation assay. Free Radic Biol Med. 1999; 26: 1231–1237. https://doi.org/10.1016/s0891-5849(98)00315-3.
• [21] Singleton VL, Orthofer R, Lamuela-Raventós RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods Enzymol. 1999; 299: 152-178. https://doi.org/10.1016/S0076-6879(99)99017-1.
• [22] Kosar M, Göger F, Can Baser KH. In vitro antioxidant properties and phenolic composition of Salvia virgata Jacq. from Turkey. J Agric Food Chem. 2008; 56(7): 2369-2374. https://doi.org/10.1021/jf073516b.
• [23] Clinical and Laboratory Standards Institute M07-A9. Reference Methods for Dilution Antimicrobial Susceptibility Tests f or Bacteria That Grow Aerobically; Approved Standard- Ninth Edition, CLSI document M07-A9. 2012; 29(2).