Understanding the effect of ginger on dental pulp stem cells differentiation into chondrocyte

Year 2025, Volume: 29 Issue: 5, 1896 - 1903, 01.09.2025

Payal Pawar Ajay Kale Ramesh Bhonde Pranjali Potdar Mayuri Chavan Avinash Kharat

https://doi.org/10.12991/jrespharm.1763604

Abstract

Ginger is utilised as a plant-based anti-inflammatory and anti-arthritic medication in traditional medicine.
Human dental pulp stem cells (hDPSCs) are an ideal in vitro model for screening drugs and biological agents. This study
evaluated the effect of ginger extract on the chondrogenic differentiation of hDPSCs. Dental pulp stem cells were isolated
using explant culture method. Stem cells surface marker expression and tri-lineage differentiation of hDPSCs were
studied. The cytotoxic impact of the ginger extract on hDPSCs was measured using the MTT assay. The impact of ginger
on the differentiation of hDPSCs into chondrocytes was examined using gene expression and biochemical staining. The
surface markers CD34, CD45, and HLA-DR were negative in the hDPSCs, while CD73, CD90, and CD105 were positive.
The hDPSCs exhibited osteocyte, chondrocyte and adipocyte differentiation. The viability of hDPSC was significantly
increased at a concentration of 10 μg/ml of ginger. Ginger administration increased the amount of glycosaminoglycan
containing extracellular matrix in hDPSCs and demonstrated by Safranin O staining. Ginger extract is a suitable
candidate for cartilage repair because it promotes chondrogenic differentiation in hDPSC. Therefore, this could be
explored further in the treatment of cartilage disorders. However, further investigation to be required.

Keywords

Dental pulp , drugs screening , chondrocyte , mesenchymal stem cell , traditional medicine

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