Betanin prohibits cisplatin-induced nephrotoxicity through targeting mitochondria

Year 2019, Volume: 23 Issue: 6, 1131 - 1139, 27.06.2025

Sepideh Zununi Vahed , Mohammadreza Ardalan Rovshan Khalilov Elham Ahmadian

Abstract

Cisplatin is a common chemotherapeutic agent against a wide range of solid tumors. The clinical application of cisplatin is restricted due to its adverse effect, mainly nephrotoxicity. The aim of this study was to examine whether betanin (a natural pigment) is able to alleviate cisplatin-induced renal injury. Rats received cisplatin (10 mg/kg, single dose) after 3 consecutive days of betanin (100 mg/kg) oral gavage. Serum and urine samples were collected and kidney mitochondria were isolated from the treated groups for the further evaluations. Cisplatin-treated animals revealed altered biochemical evidence of nephrotoxicity, which were significantly improved in betanin pre-treated groups. On the other hand, betanin modulated mitochondrial parameters such as mitochondrial dehydrogenase activity, mitochondrial swelling, mitochondrial ATP content, mitochondrial depolarization, lipid peroxidation, and cytochrome c release. These data propose the possible protective role of betanin in cisplatin-associated nephrotoxicity in which the mechanism appears to be a prohibition of chemical disturbances and mitochondrial damage.

Keywords

Cisplatin , mitochondria , betanin , nephrotoxicity , kidney

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