Total phenolic content, cyclooxygenases, α-glucosidase, acetylcholinesterase, tyrosinase inhibitory and DPPH radical scavenging effects of Cornus sanguinea leaves and fruits

Year 2020, Volume: 24 Issue: 5, 623 - 631, 27.06.2025

Burak Barut Didem Şöhretoğlu

https://doi.org/10.35333/jrp.2020.217

Cited By: 6

Abstract

The aim of the present study was to investigate total phenolic content and biological effects of methanol extracts from Cornus sanguinea L. leaves (LME) and fruits (FME). Total phenolic contents, COX-1/COX-2, αglucosidase, AChE, tyrosinase inhibitory and DPPH radical scavenging effects of both extracts were investigated by using spectrophotometric methods. The total phenolic contents of LME and FME were determined as 191.14 ± 4.84 and 31.51 ± 2.68 mg GAE/g dry weight, respectively. LME inhibited COX-1 enzyme 70.71 ± 1.88% and 79.38 ± 0.92% at 50 and 100 µg/mL. LME had higher COX-1 and COX-2 inhibitory effects than that of FME. LME inhibited αglucosidase stronger than positive control, acarbose. On the other hand, both extracts showed lower AChE inhibition actions compared to positive control, galantamine. Moreover, LME had higher tyrosinase inhibitory effect than FME. Both extracts scavenged DPPH radical in a concentration-dependent manner. Also, LME had stronger scavenging effect than that of FME. To our knowledge, current work is the first report on tyrosinase, AChE, as well as COX-1 inhibitory properties of C. sanguinea. These results suggested that LME of C. sanguinea have a promising potential for the treatment of several disorders but further studies are needed to support the this assumption.

Keywords

Acetylcholinesterase , antioxidant , cyclooxygenase , Cornus sanguinea , α-glucosidase , tyrosinase

References

• [1] David L, Moldovan B, Baldea I, Olteanu D, Bolfa P, Clichici S, Filip GA. Modulatory effects of Cornus sanguinea L. mediated green synthesized silver nanoparticles on oxidative stress, COX-2/NOS2 and NFkB/pNFkB expressions in experimental inflammation in Wistar rats. Mater Sci Eng C. 2020; 110: 110709. [CrossRef]
• [2] Stankovic MS, Topuzovic MD. In vitro antioxidant activity of extracts from leaves and fruits of common dogwood (Cornus sanguinea L.). Acta Bot Gallica. 2012; 159(1): 79-83. [CrossRef]
• [3] Chamberlain DF. Cornus L. In: Davis PH (Ed.) Flora of Trukey and the East Aegean Islands, Vol IV. Edinburgh University Press, Edinburgh, 1972, pp.539-541.
• [4] Popovic Z, Smiljanić M, Kostić M, Nikić P, Janković S. Wild flora and its usage in traditional phytotherapy (Deliblato Sands, Serbia, South East Europe). Indian J Tradit Knowl. 2014; 13: 9–35.
• [5] Bulut G. Folk medicinal plants of Silivri (Istanbul, Turkey). Marmara Pharm J. 2011; 15: 25-29. [CrossRef]
• [6] Saraç DU, Özkan ZC, Akbulut S. Ethnobotanic features of Rize/Turkey province. Biol Divers Conservat. 2013; 6: 57–66.
• [7] Yousfbeyk F, Esmaiili T, Pashna Z, Hozori Z, Ghohari AR, Ostad SN, Amin GR. Antioxidant activity, total phenol and total anthocyanin contents of Cornus sanguinea L subsp australis. (C.A. Mey.) Jáv. J Med Plant. 2014; 13(49): 69- 74.
• [8] Popović Z, Bajić-Ljubičić J, Matić R, Bojović S. First evidence and quantification of quercetin derivatives in dogberries (Cornus sanguinea L.). Turk J Biochem. 2017; 42(4): 513–518. [CrossRef]
• [9] Oskay M, Sarı D. Antimicrobial screening of some Turkish medicinal plants. Pharm Biol. 2007; 45(3): 176-181. [CrossRef]
• [10] Zhang L, Lv J. Sesquiterpenoids from Artemisia argyi and their COXs inhibitory activities. Fitoterapia 2019; 139: 104372. [CrossRef]
• [11] Larsen BHV, Soelberg J, Jager AK. COX-1 inhibitory effect of medicinal plants of Ghana. S Afr J Bot. 2015; 99: 129– 131. [CrossRef]
• [12] Tamgewagh UU, Kandhare AD, Honmore VS, Kadam PP, Khedkar VM, Bodhankar SL, Rojatkar SR. Antiinflammatory and antioxidant potential of guaianolide isolated from Cyathocline purpurea: Role of COX-2 inhibition. Int Immunopharmacol. 2017; 52: 110–118. [CrossRef]
• [13] Ni M, Hu X, Gong D, Zhang G. Inhibitory mechanism of vitexin on α-glucosidase and its synergy with acarbose. Food Hydrocoll. 2020; 105: 105824. [CrossRef]
• [14] Zhao L, Wen L, Lu Q, Liu R. Interaction mechanism between α-glucosidase and a-type trimer procyanidin revealed by integrated spectroscopic analysis techniques. Int J Biol Macromol. 2020; 143: 173–180. [CrossRef]
• [15] Ragab HM, Teleb M, Haidar HR, Gouda N. Chlorinated tacrine analogs: Design, synthesis and biological evaluation of their anti-cholinesterase activity as potential treatment for Alzheimer’s disease. Bioorg Chem. 2019; 86: 557–568. [CrossRef]
• [16] Imran M, Irfan A, Ibrahim M, Assiri MA, Khalid N, Ullah S, Al-Sehem AG. Carbonic anhydrase and cholinesterase inhibitory activities of isolated flavonoids from Oxalis corniculata L. and their first-principles investigations. Ind Crop Prod. 2020; 148: 112285. [CrossRef]
• [17] Bilska A, Kobus-Cisowska J, Kmiecik D, Danyluk B, Kowalski R, Szymanowska D, Gramza-Michlowska A, Szczepaniak O. Cholinesterase inhibitory activity, antioxidative potential and microbial stability of innovative liver pâté fortified with rosemary extract (Rosmarinus officinalis). Electron J Biotechn. 2019; 40: 22–29. [CrossRef]
• [18] Sari S, Barut B, Özel A, Şöhretoğlu D. Tyrosinase inhibitory effects of Vinca major and its secondary metabolites: Enzyme kinetics and in silico inhibition model of the metabolites validated by pharmacophore modeling. Bioorg Chem. 2019; 92: 103259. [CrossRef]
• [19] Şöhretoğlu D, Sari S, Barut B, Özel A. Tyrosinase inhibition by some flavonoids: Inhibitory activity, mechanism by in vitro and in silico studies. Bioorg Chem. 2018; 81: 168–174. [CrossRef]
• [20] Popovic Z, Matic R, Bajic-Ljubicic J, Tesevic V, Bojovic S. Geographic variability of selected phenolic compounds in fresh berries of two Cornus species. Trees 2018; 32: 203-214. [CrossRef]
• [21] Kim M, Choi S, Lee P, Hur J. Neochlorogenic acid inhibits lipopolysaccharide-induced activation and proinflammatory responses in BV2 microglial cells. Neurochem Res. 2015; 40: 1792–1798. [CrossRef]
• [22] Mandour Y, Handoussa H, Swilam N, Hanafi R, Mahran L. Structural docking studies of COX-II inhibitory activity for metabolites derived from Corchorus olitorius and Vitis vinifera. Int J Food Prop. 2016; 19: 2377–2384. [CrossRef]
• [23] Comolada M, Camuesco D, Sierra S, Ballester I, Xaus J, Galvez, Zarzuelo A. In vivo quercitrin anti‐ inflammatory effect involves release of quercetin, which inhibits inflammation through down‐ regulation of the NF‐ κB pathway. Euro J Immun. 2005; 35: 584–592. [CrossRef]
• [24] Ning Z, Zhai L, Huang T, Peng J, Hu D, Xiao H, Wen B, Lin C, Zhao L, Bian Z, Identification of α-glucosidase inhibitors from cyclocarya paliurus tea leaves using UF-UPLC-Q/TOF-MS/MS and molecular docking. Food Funct. 2019; 10: 1893-1902. [CrossRef]
• [25] Truba J, Stanislawska I, Walasek M, Wieczorkowska W, Wolinski K, Buchholz T, Melzig MF, Czerwinska ME. Inhibition of digestive enzymes and antioxidant activity of extracts from fruits of Cornus alba, Cornus sanguinea subsp. hungarica and Cornus florida–A comparative study. Plants 2020; 9: 122. [CrossRef]
• [26] Bhakta HK, Park CH, Yokozawa T, Tanaka T, Jung HA, Choi JS. Potential anti-cholinesterase and β-site amyloid precursor protein cleaving enzyme 1 inhibitory activities of cornuside and gallotannins from Cornus officinalis fruits. Arch Pharm Res. 2017; 40: 836–853. [CrossRef]
• [27] An YA, Hwang JY, Lee JS, Kim YC. Cornus officinalis methanol extract upregulates melanogenesis in melan-a cells. Toxicol Res. 2015; 31(2): 165-172. [CrossRef]
• [28] Kandia S, Linton Charles A. Statistical comparative study between the conventional DPPH% spectrophotometric and dropping DPPH% analytical method without spectrophotometer: Evaluation for the advancement of antioxidant activity analysis. Food Chem. 2019; 287: 338-345. [CrossRef]
• [29] Kahkönen MP, Hopia AI, Vuorela HJ, Rauha J, Pihlaja K, Kujala TS, Heinonen M. Antioxidant activity of plant extracts containing phenolic compounds. J Agric Food Chem. 1999; 47(10): 3954-3962. [CrossRef]
• [30] de Oliveira ADT, de Miranda MDS, Jacob ITT, da Cruz Amorim CA, de Moura RO, da Silva SAS, Soares MBP, de Almeida SMV, de Lima Souza TRC, de Oliveira JF, da Silva TG, de Melo, CRL, Moreira DRM, de Lima MC. Synthesis, in vitro and in vivo biological evaluation, COX-1/2 inhibition and molecular docking study of indole-Nacylhydrazone derivatives. Bioorg Med Chem. 2018; 26: 5388-5396. [CrossRef]
• [31] Barut B, Barut EN, Engin S, Özel A, Sezen FS, Investigation of the antioxidant, α-glucosidase inhibitory, antiinflammatory, and DNA protective properties of Vaccinium arctostaphylos L. Turk J Pharm Sci. 2019; 16: 175-183. [CrossRef]
• [32] Barut EN, Barut B, Engin S, Yıldırım S, Yaşar A, Turkis S, Özel A, Sezen FS. Antioxidant capacity, antiacetylcholinesterase activity and inhibitory effect on lipid peroxidation in mice brain homogenate of Achillea millefolium. Turk J Biochem. 2017; 42: 493-502. [CrossRef]
• [33] Sellitepe HE, Doğan İS, Eroğlu G, Barut B, Özel A. Synthesis, characterization and investigation of cholinesterase enzyme inhibition and antioxidant activities of some 4-aryl-1,4-dihydropyridine derivatives. J Res Pharm. 2019; 23(4): 608-616. [CrossRef]