Formulation and activity test of ethanol extract spray preparation of celery leaves (Apium Graveolens L) as ınsecticide against mosquitoes

Year 2025, Volume: 29 Issue: 6, 2180 - 2192, 02.11.2025

Ismail Ismail , Ade Nurkhotimah Muhammad Basri Simunati Simunati , Sukriyadi Adi , Nasrullah Nasrullah Baharuddin Kordja

https://doi.org/10.12991/jrespharm.1796183

Abstract

Celery leaves (Apium graveolens L) are one type of herbal plant that contains organic compounds such as flavonoids, saponins, and tannins which are believed to contain toxins against larvae, both those that act as contact poisons and stomach poisons that act as insecticides against mosquitoes. This study aims to test the formula of 96% ethanol extract of Apium graveolens L Spray as an insecticide against mosquitoes. It begins with the selection of celery leaf and mosquito samples, then celery leaf ethanol extraction is carried out, the design of the maceration technique spray formulation, and the evaluation of the preparation physically and chemically, namely Homogeneity Test, pH, Clarity, cycle test, and Insecticide against mosquitoes. Making ethanol extract of Apium graveolens L, making spray formulation preparations with 3 types of formulation concentrations (5%, 10% and 15%). Using the post test-only control group design experimental approach (negative control/sterile water and positive control/HIT is only carried out after testing) with the manufacture of spray preparations. Statistical tests were carried out to test the differences in the effect of each concentration on the research group, positive control, and negative control. The results of the study showed that the concentration of 3 formulas was physically and chemically stable and the insecticide test on the level of mosquito mortality for FI at the 20th minute, FII at the 18th minute and FIII at the 16th minute. The conclusion is that formula III with a concentration of 15% has effective insecticide power against mosquitoes.

Keywords

Insecticide , celery leaf (Apium graveolens Linn) , mosquito

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