Biomechanical comparison of the effects of the storage temperature on tibiotarsus in Japanese quail

Abstract

The study aimed to compare the effects of different cryopreservation temperatures on mechanical properties and determine the optimal cryopreservation temperature for bones in Japanese quail. Bone biomechanical tests are getting more attention but, fresh bones are not always available for testing and have a limited lifespan. Cryopreservation of biological specimens is often needed during tissue preparation and mechanical testing. In the study, the tibiotarsi were collected from 8 weeks of age quail, and bones were divided into four groups of fresh bones; frozen at 0 ºC, frozen at -20 ºC, and frozen at -80 ºC. Frozen bones were kept in the freezer for three weeks. After three weeks, bones were subjected to a three-point bending test for biomechanical evaluation. There was no significant difference between the mechanical strength properties of fresh tibiotarsi and the tibiotarsi stored in three different storage conditions of 0ºC, -20 ºC, or -80 ºC. It was observed that cryopreservation of tibiotarsi at 0, -20, and -80 °C for up to three weeks did not negatively affect bone biomechanical properties in quail.

Keywords

• Biomechanics
• Cryopreservation
• Freezing
• Storage temperature

References

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