Determination of Opportunistic Pathogens and Antimicrobial Resistance Characterization Isolated From Rainbow Trout in Turkey

Abstract

Modern aquaculture enables effective means for intensive aquaculture production under “controllable” conditions. This rapidly growing industry, however, has experienced relatively severe disease problems owing to a lack of control of the microbiota in rearing systems. In the present study, we identified the opportunistic pathogens and some members of natural microbiota, which were obtained from our culture collection by culturable methods and characterized biochemically in addition to molecular analysis. All isolates were identified by gene sequence analysis and confirmed taxonomically in the bacterio.net database. The isolates were characterized by means of antimicrobial susceptibilities by broth microdilution method analysis, and the resistance gene determinants were screened by PCR analysis. A total of 14 species were identified with high genetic similarity in the GenBank database. MIC results showed that bacteria have heterogeneous characteristics for the susceptibility of an agent into the genus, and species have high MIC values for sulfamethoxazole, trimethoprim, and ampicillin comparing to other agents. A total of 13 different resistance genes were determined in the bacteria, and some of them have multiple resistance genes up to five genes.

Keywords

• Opportunistic pathogens,Microbial pathogenesis,Antimicrobial resistance genes,Natural Microbiota

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