Easy Isolation, Propagation, Characterization and Multilineage Differentiation of Equine Amniotic Fluid Derived Stem Cells

Year 2020, Volume: 39 Issue: 2, 135 - 142, 30.12.2020

Asm Golam KIBRIA Shah NAWAZ Özlem ÖZDEN AKKAYA Tayfun DİKMEN Artay YAĞCI

https://doi.org/10.30782/jrvm.797971

Abstract

Easy harvesting and handy application of stem cells therapy want convenient and alternative source. Equine amniotic fluid derived mesenchymal stem cells make hope for scientists as of no ethical concern and of higher proliferation and differentiation potency. Equine AFSCs were isolated, cultured and propagated for characterization of potency of growth and multilineage differentiation study. Successful harvesting and optimum differentiation of equine AFSCs into osteogenic, adipogenic and neurospheres through this study could make a ground for their clinical application for various purposes. Further extensive and comprehensive molecular studies are important to conclude the precious procedures for clinical approach of amniotic fluid derived stem cells.

Keywords

amniotic fluid, equine, multilineage differentiation, stem cells

At Amniyotik Sıvı Kaynaklı Kök Hücrelerin Kolay İzolasyonu, Çoğaltılması, Karakterizasyonu ve Çoklu Farklılaştırması

Abstract

İzolasyon ve uygulama kolaylığı açısından kök hücre tedavisi alanında uygun bir alternatif hücre kaynağı gerekmektedir. At amniyotik sıvı kaynaklı kök hücreler (AASKH) proliferasyon kabiliyetleri, yüksek farklılaşma kabiliyetleri ve izolasyonunda etik sorunların olmaması bakımından bilim insanları için ön plana çıkmaktadır. Çalışmada at amniyotik sıvı kaynaklı kök hücreler izole edilmiş, kültüre edilmiş ve karakterizasyonu, çoğalma kabiliyetleri ve çoklu farklılaşma potansiyelleri incelenmiştir. AASKH’lerin başarılı izolasyonunun ardından bu hücrelerin osteojenik, adipojenik farklılaştırma ve nörosfer oluşturma bakımından optimal özellikler göstermesi bu hücre tipini birçok amaç için yapılacak klinik uygulamalarda uygun bir aday haline getirmektedir. AASKH’lerin klinik uygulamalarda kullanılabilirliğini görebilmek adına bu hücre tipi hakkında yapılacak daha detaylı moleküler çalışmalar yol gösterici olacaktır.

Keywords

amniyotik sıvı, at, çoklu farklılaştırma, kök hücre

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