Effect of Extenders Including High Concentrations Dimethyl Sulfoxide (DMSO) on Post-Thaw Rabbit Sperm Parameters

Year 2022, Volume: 41 Issue: 2, 88 - 91, 31.12.2022

Niyazi Küçük

https://doi.org/10.30782/jrvm.1125759

Abstract

Cryoprotectants have critical roles to prevent cell damages during cryopreservation. However, the adjustment of cryoprotectant concentration is also very crucial to protect cells from cryoprotectant toxicity. The present study was designed to investigate the effect of extenders including high concentration dimethyl sulfoxide (DMSO) on post-thaw rabbit sperm quality. Pooled rabbit sperm samples (n=7) were diluted and cryopreserved in extenders including 250 mmol/L Tris, 88 mmol/L citric acid, 47 mmol/L glucose, 1% sucrose and different concentrations of DMSO. The presence of high concentration DMSO (12 and 14%) in extender decreased sperm total and progressive motility (P < 0.01). The 8 and 10 percent DMSO supplementations in extender increased live sperm rates (P < 0.01). Live and intact acrosome or intact membrane sperm rates were detected higher in 8 and 10 DMSO groups (P < 0.05). When total intact membrane sperm rates were similar in all groups, total intact acrosome sperm rate was higher in 8 DMSO group compared to 12 DMSO group (P < 0.05). In conclusion, when 12 and 14 percent DMSO additions in extender adversely affected post-thaw sperm parameters, the presence of 8 percent DMSO in extender provided the highest post-thaw sperm quality.

Keywords

sperm, rabbit, cryopreservation, dimethyl sulfoxide

References

• 1. Kubovicova E, Makarevich A, Balazi A, Vasicek J, Chrenek P. Factors affecting rabbit sperm cryopreservation: A mini-review. Zygote. 2022;30(1):1-8.
• 2. Küçük N, Uçan U, Raza S, Erdoğan G, Aksoy M. Comparative efficiency of novel laparoscopic and routine vaginal inseminations with cryopreserved semen in rabbits. Reprod Domest Anim. 2021;56(8):1059-1065.
• 3. Rosato MP, Iaffaldano N. Cryopreservation of rabbit semen: Comparing the effects of different cryoprotectants, cryoprotectant-free vitrification, and the use of albümin plus osmoprotectants on sperm survival and fertility after standard vapor freezing and vitrification. Theriogenology. 2013;79:508–516.
• 4. Raza S, Uçan U, Aksoy M, Erdoğan G, Ceylan A, Serin İ. Silk protein sericin pretreatment enhances osmotic tolerance and post-thaw sperm quality but reduces the ability of sperm cells to undergo in vitro induced acrosome reaction in rabbit. Cryobiology. 2019;90:1-7.
• 5. Viudes-de-Castro MP, Vicente JS. A simple method for freezing rabbit semen with successful results on fertility and prolificity. Anim Reprod Sci. 1996;44:195-201.
• 6. Zhu Z, Fan X, Lv Y, Zhang N, Fan C, Zhang P, Zeng, W. Vitamin E analogue ımproves rabbit sperm quality during the process of cryopreservation through ıts antioxidative action. Plos One. 2015;10(12):1-16.
• 7. Hezavehei M, Sharafi M, Kouchesfahani HM, Henkel R, Agarwal A, Esmaeili V, Shahverdi A. Sperm cryopreservation: A review on current molecular cryobiology and advanced approaches. Reprod Biomed Online. 2018;37(3):327-339.
• 8. Nishijima K, Kitajima S, Matsuhisa F, Niimi M, Wang C, Fan, J. Strategies for highly efficient rabbit sperm cryopreservation. Animals. 2021;11:1220.
• 9. Curry MR, Redding BJ, Watson PF. Determination of water permeability coefficient and its activation energy for rabbit spermatozoa. Cryobiology. 1995;32:175–181.
• 10. Iaffaldano N, Di Iorio M, Rosato MP. The cryoprotectant used, its concentration, and the equilibration time are critical for the successful cryopreservation of rabbit sperm: dimethylacetamide versus dimethylsulfoxide. Theriogenology. 2012;78:1381–1389.
• 11. Küçük N, Raza S, Matsumura K, Uçan, U, Serin İ, Ceylan A, Aksoy M. Effect of different carboxylated poly l-lysine and dimethyl sulfoxide combinations on post thaw rabbit sperm functionality and fertility. Cryobiology. 2021;102:127-132.
• 12. Küçük N, Aksoy M. Effect of environmental heat stress on Kıvırcık ram sperm parameters. J Hell Vet Med Soc. 2020;71(1):2073-2080.
• 13. Siemea H, Oldenhofa H, Wolkers FW. Mode of action of cryoprotectants for sperm preservation. Anim Reprod Sci. 2016;169:2–5.
• 14. Si W, Zheng P, Li Y, Dinnyes A, Ji W. Effect of glycerol and dimethyl sulfoxide on cryopreservation of Rhesus Monkey (Macaca mulatta) sperm. Am J Primatol. 2004;62:301-306.
• 15. Siddiqui MSI, Parvin R, Giasuddin M, Chowdhury SMZH, Islam MR, Chowdhury EH. The effect of different concentrations of Dimethyl sulfoxide (DMSO) and glycerol as cryoprotectant in preserving Vero cells. Bangladesh Vet. 2016;33(1):1-7.