Arı Sütü Takviyesinin Sıçan Akciğerinde Tüketici Egzersiz Kaynaklı Oksidatif Stres Üzerine Etkileri

Abstract

Bu çalışmanın amacı, Arı Sütü (AS)’nün, tüketici egzersize bağlı oksidatif hasar üzerindeki etkilerini araştırmaktı. Çalışmayı gerçekleştirmek için sıçanları 4 gruba ayırdık: Kontrol (K), AS tedavisi ile Kontrol (K+AS), kontrol diyeti ile tüketici egzersiz (K+TE) ve AS tedavisi ile tüketici egzersiz (AS+TE). K+AS ve AS+TE gruplarındaki sıçanlar 14 gün boyunca 100 mg/kg AS diyeti ile beslendi. Deneyin 14. Gününde K+TE ve AS+TE gruplarındaki sıçanlar, tüketici yüzme egzersizine tabi tutuldu. Bu çalışmada akciğer oksidatif stres indeksi [malondialdehit (MDA)] ve antioksidan savunma sistemleri [süperoksit dismutaz (SOD), katalaz (CAT), glutatyon peroksidaz (GSHPx)] incelenmiştir. Lipid profillerini analiz etmek için kan örnekleri toplandı. AS tedavisi, toplam kolesterol (TK), düşük yoğunluklu lipoprotein (LDL) serum seviyelerini önemli ölçüde azalttı ve yüksek yoğunluklu lipoprotein (HDL) seviyesini önemli ölçüde arttırdı (P<0.05). AS takviyesi, akciğerde egzersize bağlı MDA seviyelerindeki artışları önemli ölçüde azalttı (P<0.05). AS, SOD aktivitelerinde egzersize bağlı artışı tersine çevirdi, ancak AS takviyesi CAT ve GSHPx aktivitelerini arttırdı. Sonuç olarak, Arı Sütü takviyesi akciğerdeki MDA seviyelerinin yükselmesini önleyebilir ve tüketici egzersiz sonrası akciğer antioksidan savunma sistemleri üzerinde olumlu bir etkiye sahip olabilir.

Keywords

• Arı sütü
• oksidatif stres
• antioksidan enzimler
• tüketici egzersiz

Effects of Royal Jelly Supplement on Exhaustive Exercise-Induced Oxidative Stress in Rat Lung

Abstract

The aim of this study was to investigate the effects of Royal Jelly (RJ) on exhaustive exercise-induced oxidative damage. To perform the study, we divided rats into four groups: control (C), control with RJ treatment (C+RJ), exhaustive exercise with control diet (C+E) and exhaustive exercise with RJ treatment (E+RJ). Rats in C+RJ and RJ+E groups were fed with 100 mg/kg RJ diet for 14 days. On the 14th day of the experiment, rats in the C+E and RJ+E groups were subjected to exhaustive swimming exercise. Lung oxidative stress indice [malondialdehyde (MDA)] and antioxidant defense systems [superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHPx)] were investigated in this study. Blood samples were collected for analyzing lipid profiles. Treatment of RJ significantly reduced serum levels of total cholesterol, low-density lipoprotein (LDL), and increased high-density lipoprotein (HDL) level. Royal jelly treatment remarkably decreased exercise-induced increases of MDA levels in lung. RJ inverted the exercise-induced increment in SOD activities, but RJ supplementation increased CAT and GSHPx activities. RJ a result, RJ supplementation can prevent elevations of MDA levels in the lung and may have a positive effect on the lung antioxidant defense systems after exhaustive exercise.

Keywords

• Royal jelly
• oxidative stress
• antioxidant enzymes
• exhaustive exercise
• lung

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