Inflammation Following Yo-Yo Level-1 Test with Circadian Timing: The Effect of Time-of-Day on Biomarker Responses

Year 2026, Volume: 8 Issue: 1, 37 - 45, 30.03.2026

Ayşegül Yapıcı , Engin Güneş Atabaş , Süleyman Gönülateş

https://doi.org/10.56639/jsar.1830568

https://izlik.org/JA22KG87EN

Abstract

The aim of this study is to investigate the acute effects of the Yo-Yo Intermittent Recovery Test (Level-1) administered in the morning and evening hours on performance, metabolic hormones, and inflammation parameters in soccer players. Thirteen voluntary male soc-cer players (mean age: 23.39 ± 2.5 years) from Tavasbirlik Sports Club, competing in the Denizli Super Amateur League, participated in the study. The Yo-Yo IR1 test was admin-istered to the athletes with a one-week interval in the morning (09:00–11:00) and evening (17:00–19:00). Data were collected via venous blood samples taken Pre-Test (Pre), Post-Test (Post), and 2 Hours Post-Test (2h-Post), along with lactate measurements taken 3 minutes after the completion of the test. According to the findings, the total distance covered in the evening measurements (1655.38 m) was statistically significantly higher than in the morning measurements (1363.07 m) (p<0.05). Similarly, evening post-exercise lactate val-ues (8.40 mmol/L) were significantly higher than morning values (6.39 mmol/L). Regard-ing inflammation parameters, it was determined that morning baseline CRP levels were higher than evening levels, whereas IL-1β levels showed an increase during the evening recovery phase. In conclusion, aerobic endurance performance and glycolytic system ac-tivation in soccer players increase during evening hours associated with circadian rhythms.

Keywords

soccer , circadian rhythm , yo-yo IR1 , Inflammation , Lactate , Performance

Inflammation Following Yo-Yo Level-1 Test with Circadian Timing: The Effect of Time-of-Day on Biomarker Responses

https://izlik.org/JA22KG87EN

Abstract

Bu çalışmanın amacı, futbolcularda sabah ve akşam saatlerinde uygulanan Yo-Yo Aralıklı Toparlanma Testi (Seviye-1)'in performans, metabolik hormonlar ve inflamasyon parametreleri üzerindeki akut etkilerini incelemektir. Çalışmaya, Denizli Süper Amatör Ligi’nde mücadele eden Tavasbirlik Spor Kulübü’nden 13 gönüllü erkek futbolcu (yaş ortalaması: 23,39 ± 2,5 yıl) katılmıştır. Sporculara Yo-Yo IR1 testi, bir hafta arayla sabah (09.00-11.00) ve akşam (17.00-19.00) saatlerinde uygulanmıştır. Veriler; Test Öncesi (TÖ), Test Sonrası (TS) ve Testten 2 Saat Sonra (TS2) alınan venöz kan örnekleri ve testin tamamlanmasından 3 dakika sonra alınan laktat ölçümleri aracılığıyla toplanmıştır. Elde edilen bulgulara göre, akşam ölçümlerinde kat edilen toplam mesafe (1655,38 m), sabah ölçümlerine (1363,07 m) kıyasla istatistiksel olarak anlamlı düzeyde daha yüksek bulunmuştur (p<0,05). Benzer şekilde, akşam egzersiz sonrası laktat değerleri (8,40 mmol/L), sabah değerlerinden (6,39 mmol/L) anlamlı derecede daha yüksek bulunmuştur. İnflamasyon parametreleri açısından; sabah bazal CRP düzeylerinin akşam düzeylerinden daha yüksek olduğu, IL-1β düzeylerinin ise akşam toparlanma evresinde artış gösterdiği tespit edilmiştir. Sonuç olarak, futbolcularda aerobik dayanıklılık performansı ve glikolitik sistem aktivasyonunun, sirkadiyen ritimle ilişkili olarak akşam saatlerinde artış gösterdiği belirlenmiştir.

Keywords

Futbol , Sirkadyen Ritim , yo-yo IR1 , İnflamasyon , Laktat , Performans

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