Application of Matrix-Assisted Laser Desorption Ionization in microbiological domain

Abstract

Mass spectrometry became more and more widespread and many new ionization and ion separation techniques were introduced. For instance, matrix-assisted laser desorption ionization time-of-flight mass spectrometry is a proteomics method that permits rapid and accurate identification of microbial species from positive cultures. This method applies to biomolecules as well as peptides, proteins, glycoproteins, and oligonucleotides and it is considered more cost effective than other techniques used in microbiology. The faster identification of the pathogen and the determination of antimicrobial susceptibility is a key in the management of patients with suspected infection. Various organisms can be identified by this technique including bacteria, fungi, and parasites. Bacteria are the most identified species followed by fungi and finally parasites. Here we present a scientific review that evaluates the performance of MALDI-TOF MS in identifying clinical pathogenic microorganisms.

Keywords

• Mass spectrometry
• Matrix-assisted laser desorption ionization time-of-flight mass
• Mass spectrometry
• fungi
• bacteria

Application of Matrix-Assisted Laser Desorption Ionization in microbiological domain

Öz

Mass spectrometry became more and more widespread and many new ionization and ion separation techniques were introduced. For instance, matrix-assisted laser desorption ionization time-of-flight mass spectrometry is a proteomics method that permits rapid and accurate identification of microbial species from positive cultures. This method applies to biomolecules as well as peptides, proteins, glycoproteins, and oligonucleotides and it is considered more cost effective than other techniques used in microbiology. The faster identification of the pathogen and the determination of antimicrobial susceptibility is a key in the management of patients with suspected infection. Various organisms can be identified by this technique including bacteria, fungi, and parasites. Bacteria are the most identified species followed by fungi and finally parasites. Here we present a scientific review that evaluates the performance of MALDI-TOF MS in identifying clinical pathogenic microorganisms.

Anahtar Kelimeler

• Mass spectrometry
• Matrix-assisted laser desorption ionization time-of-flight mass
• bacteria
• fungi
• parasites.

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