THE EFFECT OF QUERCETIN ON SOME HEMATOLOGICAL PARAMETERS AGAINST BISPHENOL-A EXPOSURE IN STREPTOZOCIN-INDUCED RATS

Abstract

Bisphenol-A (BPA) is an endocrine-disrupting environmental toxin widely used in the composition of plastics. Today, the widespread use of BPA in preserving and packaging food and beverages increases BPA exposure. Therefore, recent research has focused on the health effects of continuous exposure to BPA. This study aimed to investigate the protective effect of quercetin (QUE) on different hematologic variables in rats induced by the environmental toxin BPA and streptozocin (STZ). Wistar albino rats were administered BPA orally (p.o.) at 10 mg/kg and QUE intraperitoneally (i.p.) at 15 mg/kg for 14 days. STZ was administered subcutaneously (s.c.) in a single dose of 50 mg/kg at the beginning of the experiment. 72 rats were randomly selected for the experimental procedure and divided into 9 groups with 8 animals in each group. The groups were created as follows; Group 1: Control (Saline); Group 2: Corn oil (0.5 ml, solvent); Group 3: STZ (50 mg/kg); Group 4: BPA (10 mg/kg); Group 5: QUE (15mg/kg); Group 6: STZ (50 mg/kg) + QUE (15mg/kg); Group 7: BPA (10 mg/kg) + QUE (15mg/kg); Group 8: STZ (50 mg/kg) + BPA group (10 mg/kg); Group 9: STZ (50 mg/kg) + BPA (10 mg/kg) + QUE (15mg/kg). STZ and BPA-treated rats showed functional variability in all hematologic parameters. The combination of STZ and BPA significantly reduced erythrocytes, leukocytes, and their associated parameters. However, QUE treatment alone or in combination corrected the altered hematologic parameters. The results of this study demonstrated that exposure to BPA in combination with STZ may alter hematologic indices, while QUE may be a therapeutic agent to correct the altered blood profile.

Keywords

• Bisphenol-A
• Quercetin
• Streptozocin
• RBCs
• WBCs

References

1. [1] Abraham, A., Chakraborty, P., Staples, C., van der Hoeven, N., Clark, K., Mihaich, E., Woelz, J., Hentges, S. (2018). Distributions of concentrations of bisphenol A in North American and European surface waters and sediments determined from 19 years of monitoring data. Chemosphere, 201(8), 448–458.
2. [2] Hwang, M., Park, S.-J., and Lee, H.-J. (2023). Risk assessment of bisphenol a in the korean general population. Applied Sciences, 13(6), 3587.
3. [3] Ma, Q., Deng, P., Lin, M., Yang, L., Li, L., Guo, L., Zhang, L., He, M., Lu, Y., Pi, H., Zhang, Y., Yu, Z., Chen, C., and Zhou, Z. (2021). Long-term bisphenol-A exposure exacerbates diet-induced prediabetes via TLR4-dependent hypothalamic inflammation. Journal of Hazardous Materials, 402, 123926.
4. [4] Çelik, Y., and Şahin, S. (2021). Health effects of bisphenol-A as an endocrine disrupting chemical. Journal of Continuing Medical Education, 29(6), 439–445.
5. [5] Rancière, F., Botton, J., Slama, R., Lacroix, M. Z., Debrauwer, L., Charles, M. A., Roussel, R., Balkau, B., Magliano, D. J., and Vol, S. (2019). Exposure to bisphenol -A and bisphenol s and incident type 2 diabetes: A case-cohort study in the French cohort D.E.S.I.R. Environmental Health Perspectives, 127(10), 1–9.
6. [6] Azeem, M., Hanif, M., Mahmood, K., Ameer, N., Chughtai, F. R. S., and Abid, U. (2023). An insight into anticancer, antioxidant, antimicrobial, antidiabetic and anti-inflammatory effects of quercetin: a review. Polymer Bulletin, 80(1), 241–262.
7. [7] Lenzen, S. (2008). The mechanisms of alloxan- and streptozotocin-induced diabetes. Diabetologia, 51(2), 216–226.
8. [8] Amra, E. A., Abd, S. A., Raheem, E., Habib, T. N., and Aboelkhair, H. A. (2022). Protective effect of bradykinin potentiating factor on haematological parameters of diabetic male albino rats. Sohag Journal of Sciences, 114(2), 105–114.

9. [9] Moselhy, W., Ahmed, W. M. S., Moselhy, W. A., and Nabil, T. M. (2015). Bisphenol A toxicity in adult male rats: Hematological, biochemical and histopathological approach. Global Veterinaria, 14(2), 228–238.
10. [10] Anwar, M., Shousha, W. G., El-mezayen, H. A., Wadallah, R. A., El-Wassef, M., Nazif, N. M., and El-bana, M. A. (2013). Antiatherogenic effect of almond oil in streptozotocin induced diabetic rats. Journal of Applied Pharmaceutical Science, 3(10), 59–65.
11. [11] Abdelmoaty, M. A., Ibrahim, M. A., Ahmed, N. S., and Abdelaziz, M. A. (2010). Confirmatory studies on the antioxidant and antidiabetic effect of quercetin in rats. Indian Journal of Clinical Biochemistry, 25(2), 188–192
12. [12] Prud’homme, G. J., Glinka, Y., Kurt, M., Liu, W., and Wang, Q. (2017). The anti-aging protein Klotho is induced by GABA therapy and exerts protective and stimulatory effects on pancreatic beta cells. Biochemical and Biophysical Research Communications, 493(4), 1542–1547.
13. [13] Khan, D., Vasu, S., Moffett, R. C., Irwin, N., and Flatt, P. R. (2017). Influence of neuropeptide Y and pancreatic polypeptide on islet function and beta-cell survival. Biochimica et Biophysica Acta (BBA) - General Subjects, 1861(4), 749–758.
14. [14] Hajam, Y. A., Rai, S., Ghosh, H., and Basheer, M. (2020). Combined administration of exogenous melatonin and insulin ameliorates streptozotocin induced toxic alteration on hematological parameters in diabetic male Wistar rats. Toxicology Reports, 7, 353–359.
15. [15] Zhang, Q., Cui, Q., Hou, Y., Wang, H., Xu, Y., and Pi, J. (2017). The impairment of glucose-stimulated insulin secretion in pancreatic β-cells caused by prolonged glucotoxicity and lipotoxicity is associated with elevated adaptive antioxidant response. Food and Chemical Toxicology, 100, 161–167.
16. [16] Zhang, W., Meng, J., Liu, Q., Makinde, E. A., Lin, Q., and Olatunji, O. J. (2020). Shorea roxburghii leaf extract Ameliorates hyperglycemia induced abnormalities in high fat/fructose and streptozotocin induced diabetic rats. Chemistry & Biodiversity, 17(3), e1900661.
17. [17] Kolanjiappan, K., Manoharan, S., and Kayalvizhi, M. (2002). Measurement of erythrocyte lipids, lipid peroxidation, antioxidants and osmotic fragility in cervical cancer patients. Clinica Chimica Acta, 326(1–2), 143–149.
18. [18] Schönemann, A. M., Moreno Abril, S. I., Diz, A. P., and Beiras, R. (2022). The bisphenol-A metabolite MBP causes proteome alterations in male Cyprinodon variegatus fish characteristic of estrogenic endocrine disruption. Environmental Pollution, 300, 118936.
19. [19] Karnam, S. S., Ghosh, R. C., Mondal, S., and Mondal, M. (2015). Evaluation of subacute bisphenol -A toxicity on male reproductive system. Veterinary World, 8(6), 738–744.
20. [20] Moselhy, W., Ahmed, W. M. S., Moselhy, W. A., and Nabil, T. M. (2015). Bisphenol -A toxicity in adult male rats: Hematological, biochemical and histopathological approach. Global Veterinaria, 14(2), 228–238.
21. [21] Bhutada, P., Mundhada, Y., Bansod, K., Bhutada, C., Tawari, S., Dixit, P., and Mundhada, D. (2010). Ameliorative effect of quercetin on memory dysfunction in streptozotocin-induced diabetic rats. Neurobiology of Learning and Memory, 94(3), 293–302.
22. [22] Keskin, E., Dönmez, N., Kılıçarslan, G., and Kandır, S. (2016). Beneficial effect of quercetin on some haematological parameters in streptozotocin-induced diabetic rats. Bulletin of Environment, Pharmacology and Life Sciences, 5(6), 65-8.
23. [23] Kundrapu, S., and Noguez, J. (2018). Laboratory assessment of anemia. In G. S. Makowski (Ed.), Advances in Clinical Chemistry (1st ed., Vol. 83, pp. 197–225). Cambridge: Elsevier.
24. [24] Dörtbudak, M. Y., Çadırcı, M. Ş., and Karakılcık, A. Z. (2013). Effects of vitamin E and selenium on erythrocyte and platelet indices and pancreatic histopathology in experimental diabetes. Journal of Harran University Medical Faculty, 10(2), 54–59.
25. [25] Omoba, O. S., Olagunju, A. I., Akinrinlola, F. O., and Oluwajuyitan, T. D. (2022). Shallot-enriched amaranth-based extruded snack influences blood glucose levels, hematological parameters, and carbohydrate degrading enzymes in streptozotocin-induced diabetic rats. Journal of Food Biochemistry, 46(11), e14098.
26. [26] Gayathri, M., and Kannabiran, K. (2009). The effects of oral administration of an aqueous extract of Ficus bengalensis stem bark on some hematological and biochemical parameters in rats with streptozotocin-induced diabetes. Turkish Journal of Biology, 33(1), 9–13.
27. [27] Konsue, A., Picheansoonthon, C., and Talubmook, C. (2017). Fasting blood glucose levels and hematological values in normal and streptozotocin-induced diabetic rats of mimosa pudica L. extracts. Pharmacognosy Journal, 9(3), 315–322.
28. [28] M. Eid, H., and S. Haddad, P. (2017). The antidiabetic potential of quercetin: underlying mechanisms. Current medicinal chemistry, 24(4), 355–364.
29. [29] Hogan, S. P., Rosenberg, H. F., Moqbel, R., Phipps, S., Foster, P. S., Lacy, P., Kay, A.B., and Rothenberg, M. E. (2008). Eosinophils: biological properties and role in health and disease. Clinical & Experimental Allergy, 38(5), 709–750.
30. [30] Galli, F., Varani, M., Trapasso, F., Tetti, S., and Signore, A. (2022). Radiolabeling of monocytes, NK cells and dendritic cells and quality controls. Nuclear Medicine and Molecular Imaging, 299–304.
31. [31] Monie, T. P. (2017). A Snapshot of the Innate Immune System. (T. P. Monie, Ed.)The innate immune system (1st ed.). Cambridge: Academic Press.
32. [32] Ravikumar, N., and Kavitha, C. N. (2020). Immunomodulatory effect of quercetin on dysregulated Th1 / Th2 cytokine balance in mice with both type 1 diabetes and allergic asthma, 10(03), 80–87.
33. [33] Wiyono, T., Nisa, K., Handayani, S., Windarsih, A., Hayati, S. N., Wulanjati, M. P., Sholikhahb, E. N., and Pratiwi, W. R. (2023). Ameliorative effect of quercetin on pancreatic damage in rodent: a meta-analysis. Egyptian Journal of Basic and Applied Sciences, 10(1), 433–446.