The effect of fingerprint enhancement methods applied on adhesive surfaces on DNA recovery: a preliminary study

Abstract

The presence of body fluids such as blood, saliva, semen or urine during fingerprint research on the evidence taken from the crime scene makes it necessary to protect biological materials to examine the evidence in multiple ways. Therefore, it is crucial that fingerprint development (FD) techniques do not disrupt the structure of biological materials during FD procedures. In this sense, it is essential to determine whether biological material or fingerprints should be the priority during the collection of evidentiary materials, to determine the systematic order and to determine whether the FD methods to be applied cause damage to the genetic material used in the identification of individuals and to evaluate them in terms of their evidentiary quality. This study investigated the effects of the application of trace detection methods on DNA profiling processes in evidence where fingerprints and biological samples are found at the same time. In this study, blood, saliva, semen and urine samples were taken from a male individual who signed an informed consent form at the laboratory stage. The samples were applied 50 µL on the adhesive tape surface. After application, the samples were treated with crystal violet (CV) and sticky side (SS) fingerprint development chemicals suitable for the surface type. The prepared samples were dried under room conditions. After 1 day and 45 days under normal room conditions, silica-based DNA extraction was performed. After extraction, DNA quantification was performed using the fluorimetry method. In the study, biological samples with known DNA content were used to focus on DNA quantification. Among the fresh samples prepared in the study, DNA recovery was higher in the SS-treated urine, blood and saliva samples and in the CV-treated semen sample group compared to the other groups. This shows that chemical treatment of some biological samples on adhesive tape increases the efficiency of DNA recovery. When the 45-day waiting samples were compared with the control group samples, DNA recovery decreased in CV-treated urine and blood samples, while DNA recovery increased in SS-treated urine and blood samples. In semen samples, both CV and SS treatment negatively affected DNA recovery. In saliva samples, DNA recovery increased ~2-fold in the CV-treated sample group, while SS treatment caused a ~75% decrease in DNA recovery. The results show that the non-porous adhesive tape does not adversely affect the amount of DNA in terms of STR profiling of latent FD chemicals used on the surfaces and that adhesive tape treated with fingerprint enhancement chemicals can actually be used for advanced forensic genetic analyses for DNA extraction on surfaces.

Keywords

• Fingerprint Enhancement
• adhesive Surface
• DNA Extraction
• Forensic Genetics
• Biological fluids,

References

1. [1] C. Neumann and H. Stern, “Forensic Examination of Fingerprints: Past, Present, and Future,” Chance, vol. 29, no. 1, pp. 9–16, Jan. 2016, doi: 10.1080/09332480.2016.1156353.
2. [2] P. Kumar, R. Gupta, R. Singh, and O. P. Jasuja, “Effects of latent fingerprint development reagents on subsequent forensic DNA typing: A review,” J. Forensic Leg. Med., vol. 32, pp. 64–69, May 2015, doi: 10.1016/j.jflm.2015.03.002.
3. [3] S. P. Singh, “Development of Latent Finger Prints on Human Skin: A Review,” Int. J. Eng. Res. Technol., vol. 9, no. 6, pp. 1192–1198, 2020.
4. [4] B. Yamashita and M. French, “Chapter 7: Latent Print Development,” in Fingerprint Sourcebook, US Dept. of Justice, Office of Justice Programs, National Institute of Justice, 2010, pp. 1–68.
5. [5] S. M. Bleay, R. S. Croxton, and M. De Puit, Fingerprint development techniques: theory and application, First. Wiley Press, 2018.
6. [6] ICRC 2009: International Committee of Red Cross (ICRC), Missing People, DNA Analysis and Identification of Human Remains:A Guide to Best Practice in Armed Conflicts and Other Situations of Armed Violence, Second Edition. 2009.
7. [7] Y. Gülekçi, “Parmak İzi Araştırmaları,” in Suç Araştırmalarında Kriminal Yaklaşımlar, First., Akademisyen Press, 2021, pp. 185–214.
8. [8] I. Feine et al., “Acetone facilitated DNA sampling from electrical tapes improves DNA recovery and enables latent fingerprints development,” Forensic Sci. Int., vol. 276, pp. 107–110, Jul. 2017, doi: 10.1016/j.forsciint.2017.04.023.

9. [9] H. Lee, J. Yim, and Y. Eom, “Effects of fingerprint development reagents on subsequent DNA analysis,” Electrophoresis, vol. 40, no. 14, pp. 1824–1829, Jul. 2019, doi: 10.1002/elps.201800496.
10. [10] R. Shalhoub et al., “The recovery of latent fingermarks and DNA using a silicone-based casting material,” Forensic Sci. Int., vol. 178, no. 2–3, pp. 199–203, Jul. 2008, doi: 10.1016/j.forsciint.2008.04.001.
11. [11] M. Carlin, R. Nickel, K. Halstead, J. Viray, A. Hall, and A. Ehrlich, “Quantifying DNA loss in laboratory-created latent prints due to fingerprint processing,” Forensic Sci. Int., vol. 344, p. 111595, Mar. 2023, doi: 10.1016/j.forsciint.2023.111595.
12. [12] A. Gosch and C. Courts, “On DNA transfer: The lack and difficulty of systematic research and how to do it better,” Forensic Sci. Int. Genet., vol. 40, pp. 24–36, May 2019, doi: 10.1016/j.fsigen.2019.01.012.
13. [13] J. Sewell et al., “Recovery of DNA and fingerprints from touched documents,” Forensic Sci. Int. Genet., vol. 2, no. 4, pp. 281–285, Sep. 2008, doi: 10.1016/j.fsigen.2008.03.006.
14. [14] M. . Schulz and W. Reichert, “Archived or directly swabbed latent fingerprints as a DNA source for STR typing,” Forensic Sci. Int., vol. 127, no. 1–2, pp. 128–130, Jun. 2002, doi: 10.1016/S0379-0738(02)00092-0.
15. [15] D. Färber, A. Seul, H. Weisser, and M. Bohnert, “Recovery of Latent Fingerprints and DNA on Human Skin*,” J. Forensic Sci., vol. 55, no. 6, pp. 1457–1461, Nov. 2010, doi: 10.1111/j.1556-4029.2010.01476.x.
16. [16] S. Norlin, M. Nilsson, P. Heden, and M. Allen, “Evaluation of the impact of different visualization techniques on DNA in fingerprints,” J. Forensic Identif., vol. 63, no. 2, pp. 189–204, 2013.
17. [17] A. D. Solomon, M. E. Hytinen, A. M. McClain, M. T. Miller, and T. Dawson Cruz, “An Optimized DNA Analysis Workflow for the Sampling, Extraction, and Concentration of DNA obtained from Archived Latent Fingerprints,” J. Forensic Sci., vol. 63, no. 1, pp. 47–57, Jan. 2018, doi: 10.1111/1556-4029.13504.
18. [18] Z. Subhani, B. Daniel, and N. Frascione, “DNA Profiles from Fingerprint Lifts—Enhancing the Evidential Value of Fingermarks Through Successful DNA Typing,” J. Forensic Sci., vol. 64, no. 1, pp. 201–206, Jan. 2019, doi: 10.1111/1556-4029.13830.
19. [19] S. M. Goschay et al., “Finger mark development techniques within scope of ISO 17025,” in Fingerprint Source Book, Home Office Centre for Applied Science and Technology (CAST), 2012, pp. 233–289.
20. [20] Qiagen, “QIAamp DNA Micro Handbook,” Qiagen Sample and Assay Technologies, 2013. https://www.qiagen.com/us/resources/resourcedetail?id=085e6418-1ec0-45f2-89eb-62705f86f963&lan g=en (accessed Aug. 27, 2023).
21. [21] Thermo Fisher Scientific Inc., “Quant-iTTM dsDNA Assay Kits, high sensitivity (HS),” InvitrogenTM, 2006. https://www.thermofisher.com/order/catalog/product/Q33120 (accessed Aug. 27, 2023).
22. [22] H. C. Lee and R. E. Gaensslen, “Methods of latent fingerprint development,” in Advances in Fingerprint Technology, CRC Press, 2001, pp. 105–175.
23. [23] G. S. Bumbrah, R. M. Sharma, and O. P. Jasuja, “Emerging latent fingerprint technologies: a review,” Res. Reports Forensic Med. Sci., vol. 6, pp. 39–50, 2016.
24. [24] M. Wang, M. Li, A. Yu, Y. Zhu, M. Yang, and C. Mao, “Fluorescent Nanomaterials for the Development of Latent Fingerprints in Forensic Sciences,” Adv. Funct. Mater., vol. 27, no. 14, Apr. 2017, doi: 10.1002/adfm.201606243.
25. [25] P. Kanokwongnuwut, K. P. Kirkbride, H. Kobus, and A. Linacre, “Enhancement of fingermarks and visualizing DNA,” Forensic Sci. Int., vol. 300, pp. 99–105, Jul. 2019, doi: 10.1016/j.forsciint.2019.04.035.
26. [26] S. Gino and M. Omedei, “Effects of the most common methods for the enhancement of latent fingerprints on DNA extraction from forensic samples,” Forensic Sci. Int. Genet. Suppl. Ser., vol. 3, no. 1, pp. e273–e274, Dec. 2011, doi: 10.1016/j.fsigss.2011.08.133.
27. [27] C. Au, H. Jackson-Smith, I. Quinones, B. J. Jones, and B. Daniel, “Wet powder suspensions as an additional technique for the enhancement of bloodied marks,” Forensic Sci. Int., vol. 204, no. 1–3, pp. 13–18, Jan. 2011, doi: 10.1016/j.forsciint.2010.04.044.
28. [28] C. Lennard, “Fingermark detection and identification: current research efforts,” Aust. J. Forensic Sci., vol. 46, no. 3, pp. 293–303, Jul. 2014, doi: 10.1080/00450618.2013.839743.
29. [29] T. Spear, N. Khoshkebari, J. Clark, and M. Murphy, “Summary of experiments investigating the impact of fingerprint processing and fingerprint reagents on PCR-based DNA typing profiles,” 2015.
30. [30] C. Roux, K. Gill, J. Sutton, and C. Lennard, “A further study to investigate the effect of fingerprint enhancement techniques on the DNA analysis of bloodstains,” J. Forensic Identif., vol. 49, no. 4, pp. 357–376, 1999.
31. [31] C. Stein, S. Kyeck, and C. Henssge, “DNA Typing of Fingerprint Reagent Treated Biological Stains,” J. Forensic Sci., vol. 41, no. 6, pp. 1012–1017, Nov. 1996, doi: 10.1520/JFS14039J.
32. [32] H. C. Lee; et al., “Effect of Presumptive Test, Latent Fingerprint and Some Other Reagents and Materials on Subsequent Serological Identification, Genetic Marker and DNA Testing in Bloodstains,” J. Forensic Identif., vol. 39, no. 6, pp. 339–358, 1989.
33. [33] M. Azoury, A. Zamir, C. Oz, and S. Wiesner, “The Effect of 1,2-Indanedione, a Latent Fingerprint Reagent on Subsequent DNA Profiling,” J. Forensic Sci., vol. 47, no. 3, pp. 586–588, May 2002, doi: 10.1520/JFS2001150.
34. [34] C. Frégeau, O. Germain, and R. Fourney, “Fingerprint Enhancement Revisited and the Effects of Blood Enhancement Chemicals on Subsequent Profiler Plus TM Fluorescent Short Tandem Repeat DNA Analysis of Fresh and Aged Bloody Fingerprints,” J. Forensic Sci., vol. 45, no. 2, pp. 354–380, Mar. 2000, doi: 10.1520/JFS14688J.
35. [35] A. Zamir, C. Oz, and B. Geller, “Threat Mail and Forensic Science: DNA Profiling from Items of Evidence After Treatment with DFO,” J. Forensic Sci., vol. 45, no. 2, pp. 445–446, Mar. 2000, doi: 10.1520/JFS14704J.
36. [36] M. K. Balogh, J. Burger, K. Bender, P. M. Schneider, and K. W. Alt, “STR genotyping and mtDNA sequencing of latent fingerprint on paper,” Forensic Sci. Int., vol. 137, no. 2–3, pp. 188–195, Nov. 2003, doi: 10.1016/j.forsciint.2003.07.001.
37. [37] P. Leemans, A. Vandeput, N. Vanderheyden, J.-J. Cassiman, and R. Decorte, “Evaluation of methodology for the isolation and analysis of LCN-DNA before and after dactyloscopic enhancement of fingerprints,” Int. Congr. Ser., vol. 1288, pp. 583–585, Apr. 2006, doi: 10.1016/j.ics.2005.09.079.
38. [38] P. Tozzo, A. Giuliodori, D. Rodriguez, and L. Caenazzo, “Effect of Dactyloscopic Powders on DNA Profiling From Enhanced Fingerprints,” Am. J. Forensic Med. Pathol., vol. 35, no. 1, pp. 68–72, Mar. 2014, doi: 10.1097/PAF.0000000000000081.
39. [39] A. Zamir, E. Springer, and B. Glattstein, “Fingerprints and DNA: STR Typing of DNA Extracted from Adhesive Tape after Processing for Fingerprints,” J. Forensic Sci., vol. 45, no. 3, pp. 687–688, May 2000, doi: 10.1520/JFS14749J.