THE INVESTIGATION OF THE ACTION MECHANISM OF AMMONIUM PYRROLIDINE DITHIOCARBAMATE ON RAT BLADDER SMOOTH MUSCLE CONTRACTION-RELAXATION RESPONSES

Abstract

Ammonium pyrrolidine dithiocarbamate (PDTC), the specific inhibitor of NF-κB, is a thiol compound with anti-viral, anti-apoptotic, anti-inflammatory and antioxidant effects proven in many studies. There is not sufficient literature in what fashion that APDTC has produced these effects. We aimed to investigate the action mechanism of APDTC in rat bladder smooth muscle in our vitro study. In our study, 70 Wistar albino male rats were used. The rats were euthanized by cervical dislocation under the anesthesia and then abdomens were opened and bladders were isolated. After the removal of the connective tissues around the bladders, they were placed in the organ bath containing Krebs solution. KCl, ACh, APDTC, Atropine, Phentolamine, Propranolol, Nifedipine and TEA were applied to the bath with an appropriate protocol. The obtained data were evaluated with Kruskal Wallis and Mann-Whitney U tests. ACh induced bladder produced APDTC relaxation response in its own smooth muscle. Cholinergic receptor blocker Atropine, β-adrenergic receptor blocker Propranolol, L-type calcium channel blocker Nifedipine and atropine+phentolamine+propranolol mix have not changed the relaxation response that APDTC produced. In the presence of potassium channel blocker TEA, APDTC produced contraction response, but this was not a significantly important grade response. In the presence of α-adrenergic receptor blocker Phentolamine, APDTC produced significant contractile response. As a result, we think that APDTC shows its effect on rat bladder smooth muscle via α-adrenergic receptors and NANK system.

Keywords

• adrenergic
• bladder
• calcium channels
• APDTC
• cholinergic
• NANK system
• potassium channels
• TRP

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