PRELIMINARY STUDY ON DETERMINATION OF DNA DAMAGE OF Cyprinus carpio BY SINGLE CELL GEL ELECTROPHORESIS IN IŞIKLI LAKE, DENIZLI

Abstract

In this study, DNA damage was examined in blood samples taken from Cyprinus carpio between October 2019 and December 2019. The aim of this study is to determine single strand breaks in DNA by single cell gel electrophoresis (comet analysis). Different concentrations of Methyl methanesulfonate (MMS) was applied in vitro conditions and pollution of lake was evaluated on a cell basis. DNA damage was found to be less than the concentrations of MMS applied in October 2019. In December 2019, DNA damage was found to be less than on the basis of cells compared to different concentrations of MMS. Average Arbitrary Units (AAU) was calculated. Accordingly, the samples taken in October 2019 AAU values were obtained 1,4044±0,2725, after MMS application the results were between 2,38±1,1637 and 2,824444±0,6556. And in December 2019, AAU values in blood samples were found to be between 298278±0,2213, and MMS values between 2,35±0,8381 and 2,884167±0,657. Comet head and tail lengths were calculated from the comet images obtained from the comet parameters. Comet tail lengths were determined as 15.784 ± 6.97 μm in October 2019 and 10.899 ± 4.75 μm in December 2019. The data obtained from results, there is not much DNA damage of C. carpio in Işıklı Lake. As a result Cyprinus carpio is an efficient organism to evaluate genotoxicity of environmental pollution and the pollution in Işıklı Lake is not at critical level.

Keywords

• DNA damage
• Single cell gel electrophoresis
• Işıklı Lake
• Cyprinus carpio

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