THE INVESTIGATION OF THE ACTION MECHANISM OF AMMONIUM PYRROLIDINE DITHIOCARBAMATE ON RAT AORTA SMOOTH MUSCLE CONTRACTION-RELAXATION RESPONSES

Year 2020, Issue: 045, 50 - 69, 31.12.2020

Hayri Dayıoğlu Ayhan Yılmaz Zeynep Keleş Fatih Alan Sinan Darcan

Abstract

Ammonium Pyrrolidine Dithiocarbamate (APDTC) is an inhibitor of nuclear factor kappa b (NF-κB). Beside of these function, its antitumoral, antioxidant, anticarcinogenic and antiviral properties and also apoptosis inhibiting effect in smooth muscle cells were determined. Our experiments aimed to investigate the mechanism of action of APDTC on rat aortic smooth muscle. Some adrenergic and cholinergic receptors, l-type Ca2+ channels and K+ channels were blocked in 7 different groups, and therefore the action mechanism of APDTC whether is used or not on which channels and receptors and the extent to which they are effective were aimed to be determined. The contraction-relaxation responses after the administration of APDTC, atropine, phentolamine, propranolol, nifedipine, tetraethylammonium (TEA) and mix (atropine+phentolamine+propranolol) on living state controlled with potassium chloride (KCl) and blocked nitric oxide (NO) synthesis with l-ng-nitro arginine methyl ester (L-NAME) in the pre-contraction-induced aortic preparations with phenylephrine were investigated. The obtained data were evaluated by kruskal wallis and mann-whitney u tests. APDTC created the relaxation response in the aortic smooth muscle. Cholinergic receptor blocker atropine, α-adrenergic receptor blocker fentolamine, β-adrenergic receptor blocker propranolol, l-type calcium channel blocker nifedipine and potassium channel blocker TEA did not alter the relaxation response of APDTC. In the mix group consisting of atropine+phentolamine+propranolol, APDTC created a significant contraction response. It has been determined that APDTC can be effective on these systems via different mechanisms.

Keywords

APDTC, aorta, non‐adrenergic non‐cholinergic (NANC) system, L-NAME, phenylephrine, adrenergic, cholinergic, calcium channels, potassium channels

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