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Year 2020, Issue: 045, 70 - 80, 31.12.2020

Abstract

References

  • [1] Vanzella, T. P., Martinez, C. B. R., & Cólus, I. M. S. (2007). Genotoxic and mutagenic effects of diesel oil water soluble fraction on a neotropical fish species. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 631(1), 36–43.
  • [2] Kulaksız, G., & Sancar, A. (2007). Nükleotid Eksizyon Onarımı ve Kanser. Türk Biyokimya Dergisi.; 32: 10.
  • [3] Dı̇kı̇lı̇taş, M., & Koçyı̇ğı̇t, A. (2010). Canlılarda “Tek Hücre Jel Elektroforez” Yöntemi ile DNA Hasar Analizi (Teknik Not): Comet Analiz Yöntemi. Harran Tarım ve Gıda Bilimleri Dergisi, 14(2), 77–89.
  • [4] Arkoosh, M. R., Casillas, E., Clemons, E., Kagley, A. N., Olson, R., Reno, P., & Stein, J. E. (1998). Effect of Pollution on Fish Diseases: Potential Impacts on Salmonid Populations. Journal of Aquatic Animal Health, 10(2), 182–190.
  • [5] Frenzilli, G., Nigro, M., & Lyons, B. (2009). The Comet assay for the evaluation of genotoxic impact in aquatic environments. Mutation Research/Reviews in Mutation Research, 681(1), 80–92.
  • [6] Yoku, B., & Çakir, D. Ü. (2002). İn vivo Oksidatif DNA Hasarı Biyomarkerı; 8-Hydroxy-2’-deoxyguanosine. Türkiye Klinikleri Journal of Medical Sciences 22(5):535-43.
  • [7] Buschini, A., Martino, A., Gustavino, B., Monfrinotti, M., Poli, P., Rossi, C., Santoro, M., Dörr, A. J. M., & Rizzoni, M. (2004). Comet assay and micronucleus test in circulating erythrocytes of Cyprinus carpio specimens exposed in situ to lake waters treated with disinfectants for potabilization. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 557(2), 119–129.
  • [8] Ostling, O., & Johanson, K. J. (1984). Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells. Biochemical and Biophysical Research Communications, 123(1), 291–298.
  • [9] Singh, N. P., McCoy, M. T., Tice, R. R., & Schneider, E. L. (1988). A simple technique for quantitation of low levels of DNA damage in individual cells. Experimental Cell Research, 175(1), 184–191.
  • [10] Balik, İ., Özkök, R., Çubuk, H., & Uysal, R. (2004). Investigation of some biological characteristics of the silver crucian carp, Carassius gibelio (Bloch 1782) population in Lake Eğirdir. Turkish Journal of Zoology, 28(1), 19–28.
  • [11] Apaydın Yağcı, M., Yağcı, A., & Dölcü, B. (2016). Relationships between the physicochemical parameters and zooplankton in Eğirdir Lake (Turkey). Iranian Journal of Fisheries Sciences, 15(1), 118–132.
  • [12] Ustaoğlu, M. R., Balık, S., & Özbek, M. (2015). Işıklı Gölü (Çivril-Denizli)’nün Mollusca Faunası. Su Ürünleri Dergisi, 18(1).
  • [13] Tekin-Özan, S. (2012). Relationship of Heavy Metals in Water, Sediment and Tissues with Total Length, Weight and Seasons of Cyprinus carpio L., 1758 From. 15.
  • [14] Aygen, C. & Balık, S. (2015). Işıklı Gölü ve Kaynaklarının (Çivril-Denizli) Crustacea Faunası. Su Ürünleri Dergisi 22, 371–375
  • [15] McKelvey-Martin, V. J., Green M. H. L., Schmezer P., Pool-Zobel B. L., De Meo M. P., Collins A. (1993). The single cell gel electrophoresis assay (comet assay): A European review. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 288, 47–63.
  • [16] Azqueta, A., Shaposhnikov, S., & Collins, A. R. (2009). DNA oxidation: Investigating its key role in environmental mutagenesis with the comet assay. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 674(1), 101–108.
  • [17] Cuce, G., Cetinkaya, S., Isitez, N., Kuccukturk, S., Sozen, M. E., Kalkan, S., Cigerci, I. H., & Demirel, H. H. (2016). Effects of curcumin on methyl methanesulfonate damage to mouse kidney. Biotechnic & Histochemistry, 91(2), 122–127.
  • [18] Kammann, U., Riggers, J. C., Theobald, N., & Steinhart, H. (2000). Genotoxic potential of marine sediments from the North Sea. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 467(2), 161–168.
  • [19] Rank, J., & Jensen, K. (2003). Comet assay on gill cells and hemocytes from the blue mussel Mytilus edulis. Ecotoxicology and Environmental Safety, 54(3), 323–329.
  • [20] Lemos, N. G., Dias, A. L., Silva-Souza, A. T., & Mantovani, M. S. (2005). Evaluation of environmental waters using the comet assay in Tilapia rendalli. Environmental Toxicology and Pharmacology, 19(2), 197–201. 4-11.
  • [21] Çok, İ., Ulutas, O. K., Okusluk, Ö., Durmaz, E. & Demir, N. (2011). Evaluation of DNA Damage in Common Carp (Cyprinus carpio L.) by Comet Assay for Determination of Possible Pollution in Lake Mogan (Ankara). The Scientific World JOURNAL 11, 1455–1461.
  • [22] Kontaş, S. & Bostanci, D. (2020). The Study on Determination of Genotoxic Damage in Fish: Capoeta banarescui. Journal of Limnology and Freshwater Fisheries Research 144–152.
  • [23] Arslan, K., Duman, F., Kanbur, M. & Akyüz, B. (2016). Kayseri İli, Pınarbaşı İlçesinde Doğal Olarak Yakalanan ve Yetiştiriciliği Yapılan Gökkuşağı Alabalıklarının (Oncorhynchus mykiss) Genotoksisite Yönünden İncelenmesi, Erciyes Üniversitesi Veteriner Fakultesi Dergisi, (13)2, 131-138.
  • [24] Collins, A. R., Dobson, V. L., Dušinská, M., Kennedy, G., & Štětina, R. (1997). The comet assay: What can it really tell us? Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 375(2), 183–193.
  • [25] Cotelle, S., & Férard, J. F. (1999). Comet assay in genetic ecotoxicology: A review. Environmental and Molecular Mutagenesis, 34(4), 246–255.
  • [26] Ulutas, O. K., Okusluk, O., Demir, N., Durmaz, E., & Cok, I. (2008). Determination of possible pollution in Lake Mogan (Ankara) by Comet assay using common carp (meth carpio L.). Toxicology Letters, Supplement (180), S204–S205.
  • [27] Akcha, F. Arzul G, Rousseau S, Bardouil M. (2008). Comet assay in phytoplankton as biomarker of genotoxic effects of environmental pollution. Mar Environ Res 6:59–61.
  • [28] Klobuear, GLV., Pavlica M., Erben R, Pape D. (2003). Application of the micronucleus and comet assays to mussel Dreissena polymorpha haemocytes for genotoxicity monitoring of freshwater environments. Aquat Toxicol 64:15–23.
  • [29] Mitchelmore C.L., Chipman J.K. (1998). DNA strand breakage in aquatic organisms and the potential value of the comet assay in environmental monitoring Mutat. Res., 399 pp. 135-147.

PRELIMINARY STUDY ON DETERMINATION OF DNA DAMAGE OF Cyprinus carpio BY SINGLE CELL GEL ELECTROPHORESIS IN IŞIKLI LAKE, DENIZLI

Year 2020, Issue: 045, 70 - 80, 31.12.2020

Abstract

In this study, DNA damage was examined in blood samples taken from Cyprinus carpio between October 2019 and December 2019. The aim of this study is to determine single strand breaks in DNA by single cell gel electrophoresis (comet analysis). Different concentrations of Methyl methanesulfonate (MMS) was applied in vitro conditions and pollution of lake was evaluated on a cell basis. DNA damage was found to be less than the concentrations of MMS applied in October 2019. In December 2019, DNA damage was found to be less than on the basis of cells compared to different concentrations of MMS. Average Arbitrary Units (AAU) was calculated. Accordingly, the samples taken in October 2019 AAU values were obtained 1,4044±0,2725, after MMS application the results were between 2,38±1,1637 and 2,824444±0,6556. And in December 2019, AAU values in blood samples were found to be between 298278±0,2213, and MMS values between 2,35±0,8381 and 2,884167±0,657. Comet head and tail lengths were calculated from the comet images obtained from the comet parameters. Comet tail lengths were determined as 15.784 ± 6.97 μm in October 2019 and 10.899 ± 4.75 μm in December 2019. The data obtained from results, there is not much DNA damage of C. carpio in Işıklı Lake. As a result Cyprinus carpio is an efficient organism to evaluate genotoxicity of environmental pollution and the pollution in Işıklı Lake is not at critical level.

References

  • [1] Vanzella, T. P., Martinez, C. B. R., & Cólus, I. M. S. (2007). Genotoxic and mutagenic effects of diesel oil water soluble fraction on a neotropical fish species. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 631(1), 36–43.
  • [2] Kulaksız, G., & Sancar, A. (2007). Nükleotid Eksizyon Onarımı ve Kanser. Türk Biyokimya Dergisi.; 32: 10.
  • [3] Dı̇kı̇lı̇taş, M., & Koçyı̇ğı̇t, A. (2010). Canlılarda “Tek Hücre Jel Elektroforez” Yöntemi ile DNA Hasar Analizi (Teknik Not): Comet Analiz Yöntemi. Harran Tarım ve Gıda Bilimleri Dergisi, 14(2), 77–89.
  • [4] Arkoosh, M. R., Casillas, E., Clemons, E., Kagley, A. N., Olson, R., Reno, P., & Stein, J. E. (1998). Effect of Pollution on Fish Diseases: Potential Impacts on Salmonid Populations. Journal of Aquatic Animal Health, 10(2), 182–190.
  • [5] Frenzilli, G., Nigro, M., & Lyons, B. (2009). The Comet assay for the evaluation of genotoxic impact in aquatic environments. Mutation Research/Reviews in Mutation Research, 681(1), 80–92.
  • [6] Yoku, B., & Çakir, D. Ü. (2002). İn vivo Oksidatif DNA Hasarı Biyomarkerı; 8-Hydroxy-2’-deoxyguanosine. Türkiye Klinikleri Journal of Medical Sciences 22(5):535-43.
  • [7] Buschini, A., Martino, A., Gustavino, B., Monfrinotti, M., Poli, P., Rossi, C., Santoro, M., Dörr, A. J. M., & Rizzoni, M. (2004). Comet assay and micronucleus test in circulating erythrocytes of Cyprinus carpio specimens exposed in situ to lake waters treated with disinfectants for potabilization. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 557(2), 119–129.
  • [8] Ostling, O., & Johanson, K. J. (1984). Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells. Biochemical and Biophysical Research Communications, 123(1), 291–298.
  • [9] Singh, N. P., McCoy, M. T., Tice, R. R., & Schneider, E. L. (1988). A simple technique for quantitation of low levels of DNA damage in individual cells. Experimental Cell Research, 175(1), 184–191.
  • [10] Balik, İ., Özkök, R., Çubuk, H., & Uysal, R. (2004). Investigation of some biological characteristics of the silver crucian carp, Carassius gibelio (Bloch 1782) population in Lake Eğirdir. Turkish Journal of Zoology, 28(1), 19–28.
  • [11] Apaydın Yağcı, M., Yağcı, A., & Dölcü, B. (2016). Relationships between the physicochemical parameters and zooplankton in Eğirdir Lake (Turkey). Iranian Journal of Fisheries Sciences, 15(1), 118–132.
  • [12] Ustaoğlu, M. R., Balık, S., & Özbek, M. (2015). Işıklı Gölü (Çivril-Denizli)’nün Mollusca Faunası. Su Ürünleri Dergisi, 18(1).
  • [13] Tekin-Özan, S. (2012). Relationship of Heavy Metals in Water, Sediment and Tissues with Total Length, Weight and Seasons of Cyprinus carpio L., 1758 From. 15.
  • [14] Aygen, C. & Balık, S. (2015). Işıklı Gölü ve Kaynaklarının (Çivril-Denizli) Crustacea Faunası. Su Ürünleri Dergisi 22, 371–375
  • [15] McKelvey-Martin, V. J., Green M. H. L., Schmezer P., Pool-Zobel B. L., De Meo M. P., Collins A. (1993). The single cell gel electrophoresis assay (comet assay): A European review. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 288, 47–63.
  • [16] Azqueta, A., Shaposhnikov, S., & Collins, A. R. (2009). DNA oxidation: Investigating its key role in environmental mutagenesis with the comet assay. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 674(1), 101–108.
  • [17] Cuce, G., Cetinkaya, S., Isitez, N., Kuccukturk, S., Sozen, M. E., Kalkan, S., Cigerci, I. H., & Demirel, H. H. (2016). Effects of curcumin on methyl methanesulfonate damage to mouse kidney. Biotechnic & Histochemistry, 91(2), 122–127.
  • [18] Kammann, U., Riggers, J. C., Theobald, N., & Steinhart, H. (2000). Genotoxic potential of marine sediments from the North Sea. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 467(2), 161–168.
  • [19] Rank, J., & Jensen, K. (2003). Comet assay on gill cells and hemocytes from the blue mussel Mytilus edulis. Ecotoxicology and Environmental Safety, 54(3), 323–329.
  • [20] Lemos, N. G., Dias, A. L., Silva-Souza, A. T., & Mantovani, M. S. (2005). Evaluation of environmental waters using the comet assay in Tilapia rendalli. Environmental Toxicology and Pharmacology, 19(2), 197–201. 4-11.
  • [21] Çok, İ., Ulutas, O. K., Okusluk, Ö., Durmaz, E. & Demir, N. (2011). Evaluation of DNA Damage in Common Carp (Cyprinus carpio L.) by Comet Assay for Determination of Possible Pollution in Lake Mogan (Ankara). The Scientific World JOURNAL 11, 1455–1461.
  • [22] Kontaş, S. & Bostanci, D. (2020). The Study on Determination of Genotoxic Damage in Fish: Capoeta banarescui. Journal of Limnology and Freshwater Fisheries Research 144–152.
  • [23] Arslan, K., Duman, F., Kanbur, M. & Akyüz, B. (2016). Kayseri İli, Pınarbaşı İlçesinde Doğal Olarak Yakalanan ve Yetiştiriciliği Yapılan Gökkuşağı Alabalıklarının (Oncorhynchus mykiss) Genotoksisite Yönünden İncelenmesi, Erciyes Üniversitesi Veteriner Fakultesi Dergisi, (13)2, 131-138.
  • [24] Collins, A. R., Dobson, V. L., Dušinská, M., Kennedy, G., & Štětina, R. (1997). The comet assay: What can it really tell us? Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 375(2), 183–193.
  • [25] Cotelle, S., & Férard, J. F. (1999). Comet assay in genetic ecotoxicology: A review. Environmental and Molecular Mutagenesis, 34(4), 246–255.
  • [26] Ulutas, O. K., Okusluk, O., Demir, N., Durmaz, E., & Cok, I. (2008). Determination of possible pollution in Lake Mogan (Ankara) by Comet assay using common carp (meth carpio L.). Toxicology Letters, Supplement (180), S204–S205.
  • [27] Akcha, F. Arzul G, Rousseau S, Bardouil M. (2008). Comet assay in phytoplankton as biomarker of genotoxic effects of environmental pollution. Mar Environ Res 6:59–61.
  • [28] Klobuear, GLV., Pavlica M., Erben R, Pape D. (2003). Application of the micronucleus and comet assays to mussel Dreissena polymorpha haemocytes for genotoxicity monitoring of freshwater environments. Aquat Toxicol 64:15–23.
  • [29] Mitchelmore C.L., Chipman J.K. (1998). DNA strand breakage in aquatic organisms and the potential value of the comet assay in environmental monitoring Mutat. Res., 399 pp. 135-147.
There are 29 citations in total.

Details

Primary Language English
Journal Section Research Articles
Authors

Hilal Çavuş This is me 0000-0001-6024-4005

Müge Gidiş This is me 0000-0002-5623-1698

Publication Date December 31, 2020
Submission Date July 8, 2020
Published in Issue Year 2020 Issue: 045

Cite

IEEE H. Çavuş and M. Gidiş, “PRELIMINARY STUDY ON DETERMINATION OF DNA DAMAGE OF Cyprinus carpio BY SINGLE CELL GEL ELECTROPHORESIS IN IŞIKLI LAKE, DENIZLI”, JSR-A, no. 045, pp. 70–80, December 2020.