ANTIBACTERIAL, ANTIFUNGAL, ANTIBIOFILM, ANTIOXIDANT, AND ANTICANCER PROPERTIES OF METHANOL EXTRACTS OF SOME ACANTHOLIMON BOISS (PLUMBAGINACEAE) SPECIES

Year 2023, Issue: 054, 149 - 163, 30.09.2023

Maside Temiz Bostancı Ali Savaş Bülbül , Cihangir Uygun Muhammed Bekmezci , Ramazan Bayat , Fatih Şen

https://doi.org/10.59313/jsr-a.1296378

Abstract

Therapeutic properties of plants have been used since the existence of mankind. To benefit from this feature, the discovery of the active components of plants has gained momentum today. One of these plant species is Acantholimon Boiss. This plant extract has antifungal, antimicrobial, and anticancer properties. In this article, antimicrobial, antifungal and antioxidant capacities and antibiofilm properties of Acantholimon Boiss plant extract on 15 bacterial and 2 fungal species were evaluated. Antimicrobial properties were evaluated by Agar Well Diffusion method. It was observed that the plants formed a zone diameter of 12-18 cm. Minimum Inhibition Concentration (MIC) and Minimum Bactericidal Concentration (MBC) tests were used to determine the minimum active property. Biofilms of almost all bacteria were reduced by 10-50% by Acantholimon species at doses of 8 mg/ml-16 mg/ml. For MCF-7 cells, the IC50 values for A. acerosum subsp. brachystachyum (Boiss. ), A. acenum var. balansae, A. armenum var. balansae and A. kotschyi were 0.143 mg/ml, 0.63 mg/ml, 0.63 mg/ml, 0.63 mg/ml, 0.63 mg/ml, 0.63 mg/ml and 0.107 mg/ml, respectively. The same doses did not show a cytotoxic effect on HUVEC cell lines. With this article, it has given important results in terms of having a wide range of bacterial studies and examining its activities in fungi and cell lines. These findings suggest that extracts of Acantholimon Boiss species may be a potential source of natural medicines and antibacterial, antifungal, antibiofilm, antioxidant, and anticancer activities may support their therapeutic use.

Keywords

Acantholimon species, Anticancer activity, Antimicrobial activity, Antioxidant activity

References

• [1] Jaleel, C.A., Sathiya, S., Karthikeyan, B., Jaleel, A., Azooz, M.M., and Iqbal, M., (2008). Antibiogram of Catharanthus roseus Extracts. Global Journal of Molecular Sciences. 3 (1), 1–07.
• [2] Saroya, A.S., (2011). Herbalism, phytochemistry and ethnopharmacology. Herbalism, Phytochemistry and Ethnopharmacology. 1–430. [3] Bagetta, G., (2012). Herbal medicines : development and validation of plant-derived medicines for human health. 478.
• [4] Saad, B., Zaid, H., Shanak, S., and Kadan, S., (2017). Introduction to Medicinal Plant Safety and Efficacy. Anti-Diabetes and Anti-Obesity Medicinal Plants and Phytochemicals. 21–55.
• [5] Pandey, M., Debnath, M., Gupta, S., and Chikara, S.K., (2011). Phytomedicine: An ancient approach turning into future potential source of therapeutics. Journal of Pharmacognosy and Phytotherapy. 3 (3), 27–37.
• [6] Kubitzki, K., (1993). Plumbaginaceae. Flowering Plants, Dicotyledons. 523–530.
• [7] Chrtek, J., (1984). P. H. Davis (ed.) Flora of turkey and the east aegean Islands. Vol. 7. Folia Geobotanica et Phytotaxonomica. 19 (3), 322–322.
• [8] Bokhary, H.A., Suleiman, A.A.A., Basalah, M.O., and Parvez, S., (1987). Chemical Composition of Desert Truffles from Saudi Arabia. Canadian Institute of Food Science and Technology Journal. 20 (5), 336–341.
• [9] Doǧan, M. and Akaydin, G., (2005). A new species of Acantholimon Boiss. sect. Glumaria Boiss. (Plumbaginaceae) from Elaziǧ, Turkey. Botanical Journal of the Linnean Society. 149 (3), 351–356. [10] Alev Ateş, M., Kaptaner İğci̇, B., Selçuk Körüklü, T., Fi̇şne, A., and Aytaç, Z., (2019). Morphologic, Palynologic, and Phylogenetic Relationships of Acantholimon Species (Plumbaginaceae) Sharing Similar Habitats. Comm. J. Biol. 3 (1), 48–52.
• [11] Parvez, M.K., Basudan, O.A., Noman, O.M., Al-Dosari, M.S., and Alqasoumi, S.I., (2020). The first bioactivity studies of Acantholimon lycopodioides from high altitude Karakoram-Himalayan desert. Saudi Journal of Biological Sciences. 27 (10), 2514–2520. [12] Pasdaran, A., Sarker, S.D., Nahar, L., and Hamedi, A., (2019). Chemical Composition, Antibacterial, Insecticidal and Anti-Oxidant Activities of Three Acantholimon Species (A. atropatanum, A. gilliatii and A. tragacanthium). The Natural Products Journal. 10 (3), 272–278.
• [13] Parvez, M.K., Basudan, O.A., Noman, O.M., Al-Dosari, M.S., and Alqasoumi, S.I., (2020). The first bioactivity studies of Acantholimon lycopodioides from high altitude Karakoram-Himalayan desert. Saudi Journal of Biological Sciences. 27 (10), 2514–2520.
• [14] Soltanian, S., Sheikhbahaei, M., Mirtadzadini, M., and Khandani, B.K., (2020). Evaluation of anticancer, antioxidant and antibacterial properties of methanol extract of three Acantholimon Boiss. species. Avicenna Journal of Phytomedicine. 10 (6), 641.
• [15] Goorani, S., Shariatifar, N., Seydi, N., Zangeneh, A., Moradi, R., Tari, B., et al., (2019). The aqueous extract of Allium saralicum R.M. Fritsch effectively treat induced anemia: experimental study on Wistar rats. Oriental Pharmacy and Experimental Medicine. 19 (4), 403–413.
• [16] Nasiri, E., Naserirad, S., Lashgari, A.P., Gazor, R., Mohammadghasemi, F., and Roushan, Z.A. (2016). Hepatoprotective effect of Acantholimon bracteatum (Girard) Boiss. on formaldehyde-induced liver injury in adult male mice. Research Journal of Pharmacognosy (RJP). 3 (3), 55–61.
• [17] Gazor, R., Asgari, M., Pasdaran, A., Mohammadghasemi, F., Nasiri, E., and Roushan, Z.A., (2017). Evaluation of Hepatoprotective Effect of Acantholimon Gilliati Eerial Part Methanolic Extract. Iranian Journal of Pharmaceutical Research : IJPR. 16 (Suppl), 135.
• [18] Tóth, R., Nosek, J., Mora-Montes, H.M., Gabaldon, T., Bliss, J.M., Nosanchuk, J.D., (2019). Candida parapsilosis: From genes to the bedside. Clinical Microbiology Reviews. 32 (2).
• [19] Mayer, F.L., Wilson, D., and Hube, B., (2013). Candida albicans pathogenicity mechanisms. 4 (2), 119–128.
• [20] Bonassoli, L.A., Bertoli, M., and Svidzinski, T.I.E., (2005). High frequency of Candida parapsilosis on the hands of healthy hosts. Journal of Hospital Infection. 59 (2), 159–162.
• [21] Tóth, R., Nosek, J., Mora-Montes, H.M., Gabaldon, T., Bliss, J.M., Nosanchuk, J.D., et al. (2019) Candida parapsilosis: From genes to the bedside. Clinical Microbiology Reviews. 32 (2).
• [22] Angmo, K., Adhikari, B.S., and Rawat, G.S., (2012). Changing aspects of traditional healthcare system in western Ladakh, India. Journal of Ethnopharmacology. 143 (2), 621–630.
• [23] Abbas, Z., Khan, S.M., Alam, J., Khan, S.W., and Abbasi, A.M., (2017). Medicinal plants used by inhabitants of the Shigar Valley, Baltistan region of Karakorum range-Pakistan. Journal of Ethnobiology and Ethnomedicine. 13 (1), 1–15.
• [24] Parvez, M.K., Basudan, O.A., Noman, O.M., Al-Dosari, M.S., and Alqasoumi, S.I., (2020). The first bioactivity studies of Acantholimon lycopodioides from high altitude Karakoram-Himalayan desert. Saudi Journal of Biological Sciences. 27 (10), 2514–2520.
• [25] Doğan, S., Turan, P., Doğan, M., Arslan, O., and Alkan, M., (2007). Partial characterization of peroxidase from the leaves of thymbra plant (Thymbra spicata L. var. spicata). European Food Research and Technology. 225 (5–6), 865–871.
• [26] Cullen, J. (James), Knees, Sabina., Cubey, H.S., and Shaw, J.M.H., (2011). The European garden flora, flowering plants : a manual for the identification of plants cultivated in Europe, both out-of-doors and under glass.
• [27] Çömlekcioğlu, N. and Aygan, A., (2020). Farklı Tekniklerle Elde Edilen Hibiscus sabdariffa L. Özütünün Yağ Asitleri, Biyoaktif İçeriği ve Antimikrobiyal Aktivitesi. Turkish Journal of Agriculture - Food Science and Technology. 8 (12), 2723–2728.
• [28] Merritt, J.H., Kadouri, D.E., and O’Toole, G.A., (2006). Growing and Analyzing Static Biofilms. Current Protocols in Microbiology. 00 (1),.
• [29] Blois, M.S., (1958). Antioxidant Determinations by the Use of a Stable Free Radical. Nature. 181 (4617), 1199–1200.
• [30] Tozyılmaz, V., Bülbül, A.S., and Ceylan, Y., (2020). Anadolu Florasına Ait Bazı Alyssum L. Türlerinin Antimikrobiyal, Antioksidan ve Antibiyofilm Aktivitesinin Belirlenmesi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi.