THE EFFECTS OF PLANT ORIGIN EXOSOME EXTRACTS ON A549 AND HCT116 CANCER CELL LINES

Year 2023, Issue: 007, 46 - 58, 30.06.2023

Ayşegül Demirhan , Semra Türkoğlu , Fazilet Erman

Abstract

By applying the exosomes-like nanoparticles obtained from the leaves of the Gundelia munzuriensis plant on lung cancer (A549) and colon cancer (HCT116) cell lines, the effects of plant exosomes on cancer cells were investigated. According to our findings, the exosomes incubated with HCT 116 cell lines affected the viability of the cancerous cells by 40-50% compared to control cells. On the other hand, a rate of 50% decrease in A549 cell lines was observed in 24 hours with 250 μg/mL. As a result, our study demonstrates that plant-derived exosomes may be promising therapeutic agents for future cancer treatment, and the research provides an important source for future studies.

Keywords

Nanoparticles, HCT116, A549, cancer, Gundelia munzuriensis

Supporting Institution

Finance of this research has been provided by Firat University Scientific Research Project Council of Turkey

Project Number

Project number: SYO.20.02

Thanks

We would like to thank Prof.Dr. Fikrettin ŞAHİN for allowing us to use Yeditepe University Laboratories in this study.

BİTKİ KÖKENLİ EKSOZOM EKSTRAKTLARININ A549 ve HCT116 KANSER HÜCRE HATLARI ÜZERİNE ETKİLERİ

Abstract

Gundelia munzuriensis bitkisinin yapraklarından elde edilen eksozom benzeri nanopartiküller akciğer kanseri (A549) ve kolon kanseri (HCT116) hücre hatlarına uygulanarak bitki eksozomlarının kanser hücreleri üzerindeki etkileri araştırıldı. Bulgularımıza göre, HCT 116 hücre hatları ile inkübe edilen eksozomlar, kanserli hücrelerin canlılığını kontrol hücrelerine kıyasla %40-50 oranında etkilemiştir. Öte yandan, 250 μg/mL ile 24 saatte A549 hücre hatlarında %50'lik bir azalma gözlendi. Sonuç olarak, çalışmamız bitki kaynaklı eksozomların gelecekteki kanser tedavisi için umut verici terapötik ajanlar olabileceğini göstermektedir ve araştırma gelecekteki çalışmalar için önemli bir kaynak sağlamaktadır.

Keywords

Nanopartiküller, HCT116, A549, kanser, Gundelia munzuriensis

Project Number

Project number: SYO.20.02

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