Multi-criteria decision-making approach in biological risk assessment methodology with bioRAM© program

Year 2023, Volume: 7 Issue: 2, 1801 - 1823, 29.12.2023

Ergün Eraslan Nuray Alpoğlu Akbulut

Abstract

The Multi-Criteria Decision Making approach in risk assessment with the
BioRAM © methodology enables comparison of the relative risks posed by
biological agents and analysis in the laboratory environment. The
questionnaire used in this method is based on the principle of calculating the
weights of probability and severity criteria with expert opinions using Saaty's
semantic pairwise comparison scale. The model, which assigns values
between 0 and 1 to the sub-variables of the criteria, is used as a standard scale
in biological risk assessment. In the study, biological risk assessment of the
process "Detecting Coagulase positive Staphylococcus aureus in raw milk
samples" was carried out with the BioRAM© 2022 program. According to
the program that calculates the criterion weights with 2-dimensional
graphics, the risk level through inhalation, contact, and digestion is
calculated as high risk according to the biochemical properties of the
biological agent. When applying microbiological analysis procedures, it was
calculated as high risk through contact and ingestion, medium risk through
percutaneous injury, and medium risk through inhalation. It poses a high risk
to the public through inhalation, ingestion and contact. The risk level through
percutaneous injury is calculated as medium risk. After biosafety and risk
reduction measures were taken, the risk level of the analysis was calculated
as medium risk through ingestion, contact, and inhalation. However, despite
the precautions, the risk level posed by the analysis to society is high through
contact, ingestion, and inhalation. Although good precautions were taken,
the risk level could not be brought to an acceptable level. At the end of the
study, it was recommended to increase Personal Protective Equipment
measures to bring the risk level to an acceptable level during the
manipulations of this microorganism. In addition, it has been suggested to
use molecular techniques that reduce material volume and aerosol risk
instead of traditional microbiological analyses in the manipulation of this
microorganism.

Keywords

Bioram, Probability, Severity, Multi-Criteria Decision Making

BioRAM© programıyla biyolojik risk değerlendirme metodolojisinde çok kriterli karar verme yaklaşımı

Abstract

BioRAM ©metodolojisi ile risk değerlendirmesinde Çok Kriterli Karar Verme
yaklaşımı biyolojik ajanların ve analizlerin oluşturduğu göreli risklerin
laboratuvar ortamında karşılaştırılmasını sağlamaktadır. Bu yöntemde kullanılan
anket Saaty’nin semantik ikili karşılaştırma ölçeği kullanılarak uzman
görüşleriyle olasılık ve şiddet kriterlerinin ağırlıklarının hesaplanması prensibine
dayanmaktadır. Kriterlerin alt değişkenlerine 0-1 arasında değerler atayan model
biyolojik risk değerlendirmesinde standart ölçek olarak kullanılmaktadır.
Çalışmada “Çiğ süt numunesinde Koagülaz pozitif Staphylococcus aureus
aranması” proseslerinin BioRAM© 2022 programıyla biyolojik risk
değerlendirmesi yapılmıştır. İki boyutlu grafiklerle kriter ağırlıklarını hesaplayan
programa göre biyolojik ajanın biyokimyasal özelliklerine göre solunum, temas,
sindirim yoluyla risk düzeyi yüksek riskli olarak hesaplanmıştır. Mikrobiyolojik
analiz prosedürleri uygulanırken temas ve sindirim yoluyla yüksek riskli,
perkütan yaralanma yoluyla orta riskli, inhalasyon yoluyla orta riskli olarak
hesaplanmıştır. Toplum için solunum, sindirim ve temas yoluyla risk düzeyi
yüksek risklidir. Perkütan yaralanma yoluyla risk düzeyi orta riskli olarak
hesaplanmıştır. Biyogüvenlik ve risk azaltma önlemleri alındıktan sonra analizin
risk düzeyi sindirim, temas ve inhalasyon yoluyla orta riskli olarak
hesaplanmıştır. Ancak önlemlere rağmen analizin toplum için oluşturduğu risk
düzeyi temas, sindirim ve solunum yoluyla yüksek risklidir. İyi düzeyde önlemler
alınmasına rağmen risk düzeyi kabul edilebilir düzeye getirilememiştir.
Çalışmanın sonunda bu mikroorganizmanın manipülasyonları sırasında risk
düzeyinin kabul edilebilir düzeye getirilmesi için Kişisel Koruyucu Donanım
önlemlerinin artırılması önerilmiştir. Ayrıca bu mikroorganizmanın
manipülasyonlarında geleneksel mikrobiyolojik analizlerinin yerine malzeme
hacmini ve aerosol riskini azaltan moleküler tekniklerin kullanılması önerilmiştir.

Keywords

BioRAM©, Olasılık, Şiddet, Çok Kriterli Karar Verme

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