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Enzyme Immobilization Onto Carbon Fiber Electrodes by Electrochemical Polymerization

Year 2020, Volume: 10 Issue: 1, 82 - 87, 25.06.2020

Ayşe Elif Böyükbayram

Abstract

New enzyme electrodes were fabricated by using carbon fiber as electrode substrate. Electrodes were obtained by immobilization of
invertase in the conductive polypyrrole matrix during electrochemical polymerization onto laboratory–made carbon fiber electrodes.
Maximum reaction rate, (Vmax) and substrate affinity, (Km), of immobilized enzyme were determined as 56.7 μmol min-1 electrode-1
and 1.57 mM respectively. The effect of conditions on enzyme activity was investigated. It was found that the optimum temperature,
optimum pH value, linear working range are 30 ºC, pH 5.0 and 0.0025−0.0200 M respectively. The electrodes were examined for
operational stability. The results were compared with the previous studies in which invertase was immobilized onto platinum substrate.

Keywords

Carbon fiber electrode Conducting polymer

References

  • Ak, M., Yildiz, H.B., Toppare, L. 2014. Enzyme immobilization in a photosensitive conducting polymer bearing azobenzene in the main chain. Polym. Bull., 71:1827–1841. https://doi. org/10.1007/s00289-014-1157-7
  • Aydar, S., Böyükbayram, A.E., Şendur, M., Toppare, L. 2011. Immobilization of Invertase in DAD Type Polymers: Combination of Benzothiadiazole Acceptor Unit and 3,4-Ethylenedioxythiophene and Thiophene Donor Units. J. Macromol. Sci. A, 48: 855–861. https://doi.org/10.1080/1060 1325.2011.614826

Elektrokimyasal Polimerizasyonla Karbon Fiber Elektrotlara Enzim Tutuklaması

Year 2020, Volume: 10 Issue: 1, 82 - 87, 25.06.2020

Ayşe Elif Böyükbayram

Abstract

Elektrot malzemesi olarak karbon fiber kullanılarak yeni enzim elektrotları hazırlandı. Elektrotlar, laboratuvarda hazırlanan karbon
fiber elektrotlar üzerine elektrokimyasal polimerizasyon esnasında invertazın iletken polipirol matrisinde tutuklanmasıyla elde edildi.
Tutuklanan enzimin kinetik parametreleri, Vmax (maksimum reaksiyon hızı) ve Km (substratın enzim ilgisi) 56,7 μmol dak-1 elektrot-1
ve 1,57 mM olarak elde edildi. Reaksiyon koşullarının enzim aktivitesine etkisi incelendi. Tutuklanmış enzimin optimum sıcaklığı,
optimum pH’ı ve doğrusal çalışma aralığı 30 ºC, pH 5, ve 0,0025−0,0200 M olarak bulundu. Elektrotların ardışık stabilitesi saptandı.
Sonuçlar invertazın polipirol kaplı platin elektrotlara tutuklandığı daha önceki çalışmalarla karşılaştırıldı.

Keywords

Karbon fiber elektrot İletken polimer

References

  • Ak, M., Yildiz, H.B., Toppare, L. 2014. Enzyme immobilization in a photosensitive conducting polymer bearing azobenzene in the main chain. Polym. Bull., 71:1827–1841. https://doi. org/10.1007/s00289-014-1157-7
  • Aydar, S., Böyükbayram, A.E., Şendur, M., Toppare, L. 2011. Immobilization of Invertase in DAD Type Polymers: Combination of Benzothiadiazole Acceptor Unit and 3,4-Ethylenedioxythiophene and Thiophene Donor Units. J. Macromol. Sci. A, 48: 855–861. https://doi.org/10.1080/1060 1325.2011.614826
There are 2 citations in total.

Details

Primary Language English
Journal Section Research Articles
Authors

Ayşe Elif Böyükbayram

Publication Date June 25, 2020
Published in Issue Year 2020 Volume: 10 Issue: 1

Cite

APA Böyükbayram, A. E. (2020). Enzyme Immobilization Onto Carbon Fiber Electrodes by Electrochemical Polymerization. Karaelmas Fen Ve Mühendislik Dergisi, 10(1), 82-87.
AMA Böyükbayram AE. Enzyme Immobilization Onto Carbon Fiber Electrodes by Electrochemical Polymerization. Karaelmas Fen ve Mühendislik Dergisi. June 2020;10(1):82-87.
Chicago Böyükbayram, Ayşe Elif. “Enzyme Immobilization Onto Carbon Fiber Electrodes by Electrochemical Polymerization”. Karaelmas Fen Ve Mühendislik Dergisi 10, no. 1 (June 2020): 82-87.
EndNote Böyükbayram AE (June 1, 2020) Enzyme Immobilization Onto Carbon Fiber Electrodes by Electrochemical Polymerization. Karaelmas Fen ve Mühendislik Dergisi 10 1 82–87.
IEEE A. E. Böyükbayram, “Enzyme Immobilization Onto Carbon Fiber Electrodes by Electrochemical Polymerization”, Karaelmas Fen ve Mühendislik Dergisi, vol. 10, no. 1, pp. 82–87, 2020.
ISNAD Böyükbayram, Ayşe Elif. “Enzyme Immobilization Onto Carbon Fiber Electrodes by Electrochemical Polymerization”. Karaelmas Fen ve Mühendislik Dergisi 10/1 (June 2020), 82-87.
JAMA Böyükbayram AE. Enzyme Immobilization Onto Carbon Fiber Electrodes by Electrochemical Polymerization. Karaelmas Fen ve Mühendislik Dergisi. 2020;10:82–87.
MLA Böyükbayram, Ayşe Elif. “Enzyme Immobilization Onto Carbon Fiber Electrodes by Electrochemical Polymerization”. Karaelmas Fen Ve Mühendislik Dergisi, vol. 10, no. 1, 2020, pp. 82-87.
Vancouver Böyükbayram AE. Enzyme Immobilization Onto Carbon Fiber Electrodes by Electrochemical Polymerization. Karaelmas Fen ve Mühendislik Dergisi. 2020;10(1):82-7.
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