Abstract
Ektoin EKT , halofil bakterilerin stres faktörlerine karşı olarak ürettiği bir ozmoprotektandır. Bu amino asit türevi madde, doğal proseslere müdahale etmeden yüksek konsantrasyonlarda hücreler içinde birikebilir ve hücrenin radyasyona ya da ozmotik strese karşı korunmasına yardımcı olabilir. EKT, farklı alanlardaki yoğun uygulamalarından dolayı bilim adamlarının ilgisini kazanmaktadır. Bu çalışmada, insan lenfosit hücrelerini kullanarak, EKT’nin genotoksik ve anti-genotoksik potansiyellerini incelemeyi amaçladık. Sonuçlar, EKT’nin güçlü antigenotoksik özelliğe sahip olduğunu gösterdi. Ayrıca bulgularımız NaN3’e maruz bırakılan insan lenfosit hücrelerinde gözlemlenen koruyucu etkinin, EKT’nin antioksidatif özellikleriyle ilişkili olabileceğini düşündürmektedir
Keywords
References
- Abdel-Aziz, H., Wadi, W., Abdallah, DM., Lentzen, G., Khayyal, MT. 2013. Novel effects of ectoine, a bacteria- derived natural tetrahydropyrimidine, in experimental colitis. Phytomedicine, 20: 585–591.
- Arakawa, T., Timasheff, S.N. 1985. The stabilization of proteins by osmolytes. Biophys. J., 47 (3): 411-414.
- Becker, J., Schäfer, R., Kohlstedt, M., Harder, BJ., Borchert, N.S., Stöveken, N., Bremer, E., Wittmann, C. 2013. Systems metabolic engineering of Corynebacterium glutamicum for production of the chemical chaperone ectoine. Microb. Cell Fact., 12 (1): 110.
- Botta, C., Di Giorgio, C., Sabatier, A. S., De Méo, M. 2008. Genotoxicity of visible light (400–800nm) and photoprotection assessment of ectoin, l-ergothioneine and mannitol and four sunscreens. J. Photoch. Photobio. B., 91 (1): 24-34.
- Bownik, A., Stępniewska, Z. 2015a. Protective effects of ectoine on behavioral, physiological and biochemical parameters of Daphnia magna subjected to hydrogen peroxide. Comp. Biochem. Phys. C., 170: 38-49.
- Bownik, A., Stępniewska, Z. 2015b. Protective effects of bacterial osmoprotectant ectoine on bovine erythrocytes subjected to staphylococcal alpha-haemolysin. Toxicon, 99: 130-135.
- Bownik, A., Stępniewska, Z. 2016. Ectoine as a promising protective agent in humans and animals. Arh. Hig. Rada Toksiko., 67 (4): 260-264.
- Tice, R.R., Agurell, E., Anderson, D., Burlinson, B., Hartmann, A., Kobayashi, H., Miyamae, Y., Rojas, E., Ryu, J.C., Sasaki, Y.F. 2000. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ. Mol. Mutagen., 35: 206-221.
- Wei, L., Wedeking, A., Buttner, R., Kalff, JC., Tolba, RH., van Echten-Deckert, G. 2009. A natural tetrahydropyrimidine protects small bowel from cold ischemia and subsequent warm in vitro reperfusion injury. Pathobiology, 76: 212–220.
- Yao, CL., Lin, Y.M., Mohamed, MS., Chen, JH. 2013. Inhibitory effect of ectoine on melanogenesis in B16-F0 and A2058 melanoma cell lines. Biochem. Eng. J., 78: 163-169.
- Yu, I., Jindo, Y., Nagaoka, M. 2007. Microscopic understanding of preferential exclusion of compatible solute ectoine: direct interaction and hydration alteration. J. Phys. Chem. B, 111 (34): 10231-10238.
- Zhang, L., Wang, Y., Zhang, C., Wang, Y., Zhu, D., Wang, C., Nagata, S. 2006. Supplementation effect of ectoine on thermostability of phytase. J. Biosci. Bioeng., 102: 560–563.
- Sydlik, U., Gallitz, I., Albrecht, C., Abel, J., Krutmann, J., Unfried, K. 2009. The compatible solute ectoine protects against nanoparticle-induced neutrophilic lung inflammation. Am. J. Respir. Crit. Care Med., 180: 29–35.
Abstract
Ectoine ECT is an osmoprotectant produced by halophilic bacteria in response to stressful factors. This amino acid derivative material can accumulate inside cells at high concentrations without interfering with natural processes and can protect the cell against radiation or osmotic stress. ECT is receiving increasing awareness by the scientific community because of its multiple applications. In the present study, we aimed to investigate the genotoxic and anti-genotoxic potencies of ECT by using human lymphocytes cells. The results showed that ECT has strong anti-genotoxic properties. Also, our findings suggest that protective effects of ECT in human lymphocytes cells subjected to NaN3 may be related to antioxidative properties of the ECT.
Keywords
References
- Abdel-Aziz, H., Wadi, W., Abdallah, DM., Lentzen, G., Khayyal, MT. 2013. Novel effects of ectoine, a bacteria- derived natural tetrahydropyrimidine, in experimental colitis. Phytomedicine, 20: 585–591.
- Arakawa, T., Timasheff, S.N. 1985. The stabilization of proteins by osmolytes. Biophys. J., 47 (3): 411-414.
- Becker, J., Schäfer, R., Kohlstedt, M., Harder, BJ., Borchert, N.S., Stöveken, N., Bremer, E., Wittmann, C. 2013. Systems metabolic engineering of Corynebacterium glutamicum for production of the chemical chaperone ectoine. Microb. Cell Fact., 12 (1): 110.
- Botta, C., Di Giorgio, C., Sabatier, A. S., De Méo, M. 2008. Genotoxicity of visible light (400–800nm) and photoprotection assessment of ectoin, l-ergothioneine and mannitol and four sunscreens. J. Photoch. Photobio. B., 91 (1): 24-34.
- Bownik, A., Stępniewska, Z. 2015a. Protective effects of ectoine on behavioral, physiological and biochemical parameters of Daphnia magna subjected to hydrogen peroxide. Comp. Biochem. Phys. C., 170: 38-49.
- Bownik, A., Stępniewska, Z. 2015b. Protective effects of bacterial osmoprotectant ectoine on bovine erythrocytes subjected to staphylococcal alpha-haemolysin. Toxicon, 99: 130-135.
- Bownik, A., Stępniewska, Z. 2016. Ectoine as a promising protective agent in humans and animals. Arh. Hig. Rada Toksiko., 67 (4): 260-264.
- Tice, R.R., Agurell, E., Anderson, D., Burlinson, B., Hartmann, A., Kobayashi, H., Miyamae, Y., Rojas, E., Ryu, J.C., Sasaki, Y.F. 2000. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ. Mol. Mutagen., 35: 206-221.
- Wei, L., Wedeking, A., Buttner, R., Kalff, JC., Tolba, RH., van Echten-Deckert, G. 2009. A natural tetrahydropyrimidine protects small bowel from cold ischemia and subsequent warm in vitro reperfusion injury. Pathobiology, 76: 212–220.
- Yao, CL., Lin, Y.M., Mohamed, MS., Chen, JH. 2013. Inhibitory effect of ectoine on melanogenesis in B16-F0 and A2058 melanoma cell lines. Biochem. Eng. J., 78: 163-169.
- Yu, I., Jindo, Y., Nagaoka, M. 2007. Microscopic understanding of preferential exclusion of compatible solute ectoine: direct interaction and hydration alteration. J. Phys. Chem. B, 111 (34): 10231-10238.
- Zhang, L., Wang, Y., Zhang, C., Wang, Y., Zhu, D., Wang, C., Nagata, S. 2006. Supplementation effect of ectoine on thermostability of phytase. J. Biosci. Bioeng., 102: 560–563.
- Sydlik, U., Gallitz, I., Albrecht, C., Abel, J., Krutmann, J., Unfried, K. 2009. The compatible solute ectoine protects against nanoparticle-induced neutrophilic lung inflammation. Am. J. Respir. Crit. Care Med., 180: 29–35.
Details
| Primary Language | Turkish |
|---|---|
| Journal Section | Research Article |
| Authors | |
| Publication Date | January 1, 2018 |
| Published in Issue | Year 2018 Volume: 8 Issue: 1 |