Optimization of physical factors affecting the production of the α-amylase from a newly isolated Bacillus sp. M10 strain

Year 2017, Volume: 7 Issue: 1, 23 - 30, 01.01.2017

Elif Demirkan Tuba Sevgi Merve Başkurt

Abstract

In this study, sixty bacteria isolated from soil samples that come from 15 different cities of Turkey. The four isolates which had a higher ratio of clearing zone were determined to be α-amylase positive, and were investegated for morphological and physiological. All of these were defined as Bacillus. Enzyme production capacity of these 4 strain have been tested. The bacteria which has the highest α-amylase activity was selected as a Bacillus strain and these strain was named as Bacillus sp. M-10. It's investigated that physical factors which are affecting the α-amylase production of Bacillus sp. M-10. For this purpose, temperature, pH, aeration, inoculum size and inoculum age have been tested as physical parameters. In starchy medium, 37°C temperature, pH 7.0, 150 rpm for aeration, 2.5 ml inoculation size and 2 days for inoculation age were the optimum rate for maximum α-amylase production of Bacillus sp. M-10 strain. In order to enhance the production of α-amylase, medium was modified by our, and the enzyme activity was found 30 U/mL at the hour of 48. In modified environment, enzyme activity was increased about 42% rate.

Keywords

Isolation, Bacillus sp., α-amylase, Physical factors

Yeni İzole Edilen Bacillus sp. M-10 Suşundan α-Amilazın Üretimini Etkileyen Fiziksel Faktörlerin Optimizasyonu

Abstract

Bu çalışmada Türkiye’ nin 15 farklı ilinden temin edilen toprak örneklerinden 60 bakteri izole edilmiştir. α-Amilaz pozitif olarak belirlenen en geniş açık zon çapına sahip 4 bakterinin morfolojik ve fizyolojik özellikleri araştırılmış ve hepsinin Bacillus cinsine ait olduğu saptanmıştır. 4 suşun enzim üretim kapasiteleri test edilmiştir. En yüksek α-amilaz aktivitesine sahip Bacillus suşu seçilmiş ve bu suş, Bacillus sp. M-10 olarak adlandırılmıştır. Bacillus sp. M-10’un α-amilaz üretimini etkileyen bazı fiziksel faktörlerin etkisi araştırılmıştır. Bu amaçla, sıcaklık, pH, havalandırma, inokulüm miktarı ve inokulüm yaşı gibi fiziksel parametreler denenmiştir. Bacillus sp. M-10 suşunun maksimum α-amilaz üretimi, nişastalı ortamda, 37°C sıcaklıkta, pH 7,0, havalandırma 150 rpm, inokülasyon miktarının %2.5 v/v ve inokülasyon yaşının 2 gün olduğu değerlerde optimum düzeydedir. Tarafımızdan modifiye edilen ortam, daha yüksek enzim aktivitesi elde etmek için en uygun ortam olup, enzim aktivitesi 48. saatte 30 U/ mL olarak bulunmuştur. Modifiye edilen ortamda enzim aktivitesinde yaklaşık % 42 oranında artış gözlenmiştir

Keywords

Bacillus sp., İzolasyon, Fiziksel faktörler, α-amilaz

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