Kahramanmaraş Topraklarından İzole Edilen Bacillus sp. Tarafından Alfa-Amilaz Üretimi ve Karakterizasyonu

Year 2015, Volume: 5 Issue: 2, 68 - 74, 01.06.2015

Ashabil Aygan

Abstract

Bacillus sp. A-3 Kahramanmaraş topraklarından izole edilmiştir. Suş 5.0-7.0 pH aralığında enzim sentezini gerçekleştirirken, maksimum amilolitik aktivite 37oC sıcaklık ve 6.5 pH değerinde gözlenmiştir. SDS-PAGE analizinde enzime ait 53.94 kDa ağırlığında tek bant elde edilmiştir. Enzimin optimum pH’sı 6.0-6.5 olarak belirlenmiştir. Enzimin maksimum rölatif aktivite gösterdiği sıcaklık değeri 35oC olup ve 25 ile 65oC arasında ortalama %68 bir rölatif aktiviteye sahiptir. Enzim 40oC ye kadar termostabil bir özellik sergilemiştir. Kimyasalların etkisi açısından ise en yüksek kalan aktivite %93 ile KCl ve en düşük ise %0.8 ile EDTA’da gözlenmiştir. Bu özellikleri ile A-3 alfa amilazı unlu mamul sanayiinde, biyoetanol üretiminde ve hurda kâğıt endüstrisinde önemli bir yer teşkil edebilecek potansiyeldedir

Keywords

Alfa amilaz, Bacillus, Karakterizasyon, Mezofilik, Saflaştırma, Stabilite

Production and Characterization of Alfa-Amylase by Bacillus sp. Isolated from Kahramanmaraş Soils

Abstract

Bacillus sp. A-3 isolated from soils of Kahramanmaras. The maximum amylolitic activity was obtained at 37o C and pH 6.5 as the strain showed enzyme synthesis in between pH 5.0-7.0. SDS-PAGE analysis of the enzyme showed a single band which was estimated as 53.94 kDa. Optimum pH of the enzyme was determined as 6.0-6.5. Maximum relative activity of the enzyme presented was 35o C and also average 68% of relative activity was observed in between 25-65o C. Enzyme revealed a thermostable properties up to 40o C. In terms of chemicals effect, The highest remaining activity was 93% with KCl as the lowest one was 0.8% in the presence of EDTA. According to these properties of the A-3 alpha amylase has potential to have an important place in baking, bioethanol production and waste paper industries

Keywords

Bacillus, alfa amilaz, karakterizasyon, mesofilik, saflaştırma, stabilite

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