Diamid türü bir insektisit olan klorantraniliprol tarım uygulamalarında çok kullanılan, zararlılarda iyonoregülasyon sistemini bloke eden, toksik özelliği ile etkinlik gösteren bir pestisittir. Bu çalışmada klorantraniliprol, 1 ppm ve 2 ppm konsantrasyonlarının etkisinde Oreochromis niloticus solungaç dokularında iyon transport enzim aktivitelerine üzerine etklileri 7 gün süre ile araştırılmıştır. Araştırmamızda elde edilen sonuçlar solungaç dokusunda klorantraniliprol 7 gün süreyle etkisinde Na+/K+-ATPaz enzim aktivitesinin kontrol grubuna göre 1 ppm ve 2 ppm konsantrasyonlarında sırasıyla % 61 ve % 65 azalma gösterdiği belirlendi. Klorantraniliprol maruziyetinde, kontrol grubuna göre 1 ppm ve 2 ppm
konsantrasyonlarında sırasıyla Ca2+-ATPaz enzim aktivitesinde %68 ve %76, Mg2+-ATPaz enzim aktivitesinde %28 ve %44 azalma olduğu belirlendi. Çalışmanın sonuçları, Oreochromis niloticus balıklarının iyon regülasyon sisteminin, klorantraniliprol’ün etkisinde iyi bir biyobelirteç olduğunu göstermektedir.
Agrahari, S., Gopal, K. 2008. Inhibition of Na+–K+-ATPase in
different tissues of freshwater fish Channa punctatus (Bloch)
exposed to monocrotophos. Pestic. Biochem. Phys., 92(2), 57-60.
Doi:10.1016/j.pestbp.2008.06.003
Atkinson, A., Gatemby, A.O., Lowe, A.G. 1973. The
determination of inorganic orthophosphate in biological
systems. Biochim. Biophys. Acta., 320:195–204. Doi:
10.1016/0304-4165(73)90178-5
Blocksom, KA., Walters, DM., Jicha, TM., Lazorchak, JM.,
Angradi, TR., Bolgrien, DW. 2010. Persistent organic
pollutants in fish tissue in the mid-continental great rivers of
the United States. Sci. Total Environ., 408(5), 1180-1189. Doi:
10.1016/j.scitotenv.2009.11.040
Boyle, RT., Oliveira, LF., Bianchini, A., Souza, MM. 2013.
The Effects of Copper on Na+/K+-ATPase and Aquaporin
Expression in Two Euryhaline Invertebrates. Bull Environ.
Contam. Toxicol. 90:387–390. Doi:10.1007/s00128-012-0949-
4
Bradford, M. 1976. A rapid and sensitive method for the
quantitation of microgram quantities of protein utilizing the
principle of protein-dye binding. Anal. Biochem., 72:248-254.
Doi: 10.1006/abio.1976.9999
Ciacci, C., Barmo, C., Gallo, G., Maisano, M., Cappello,
T., D’Agata, A., Canesi, L. 2012. Effects of sublethal,
environmentally relevant concentrations of hexavalent
chromium in the gills of Mytilus galloprovincialis. Aqua.
Toxicol., 120, 109-118. Doi:10.1016/j.aquatox.2012.04.015
Davis, PW., Wedemeyer, GA., 1971. Na+/K+ activated ATPase
inhibition in rainbowtrout. A site for organochlorine
pesticide toxicity. Comp. Biochem. Physiol. 40,823–827.
Doi:10.1016/0305-0491(71)90157-X
Dellali, M., El Bour, N., Mahmoud, M., Patricia, A., Mahmoudi,
E. 2010. Ecolo. Indicators, 10,696.
Evans, DH., Piermarini, PM., Choe, KP. 2005. The
multifunctional fish gill: dominant site of gas exchange,
osmoregulation, acid-base regulation, and excretion of
nitrogenous waste. Physiol. Rev., 85(1), 97-177. Doi:10.1152/
physrev.00050.2003
Jorgensen, PL., Hakansson, KO. and Karlish, SJD. 2003.
Structure and mechanism of Na+/K+– ATPase: functional
sites and their interactions, Annu. Rev. Physiol., 65: 817-849.
Doi:10.1146/annurev.physiol.65.092101.142558
Lahm, GP., Selby, T. P., Freudenberger, JH., Stevenson, TM.,
Myers, BJ., Seburyamo, G., Cordova, D. 2005. Insecticidal
anthranilic diamides: a new class of potent ryanodine receptor
activators. Bioorg. Med. Chem. Lett., 15(22), 4898-4906.
Doi:10.1016/j.bmcl.2005.08.034
Lahm, GP., Cordova, D., Barry, JD. 2009. New and selective
ryanodine receptor activators for insect control. Bioorg. Med.
Chem., 17(12), 4127-4133. Doi:10.1016/j.bmc.2009.01.018
Mishra, D., Srivastav, SK., & Srivastav, AK. 2005. Effects
of the insecticide cypermethrin on plasma calcium and
ultimobranchial gland of a teleost, Heteropneustes fossilis.
Ecotoxicol. Environ. Saf., 60(2), 193-197. Doi:10.1016/j.
ecoenv.2003.12.020
Murali, M., Carvalho, MS., & Shivanandappa, T. 2020.
Oxidative stress-mediated cytotoxicity of Endosulfan is
causally linked to the inhibition of NADH dehydrogenase
and Na+, K+-ATPase in Ehrlich ascites tumor cells. Mol. Cell.
Biochem., 1-10. Doi:10.1007/s11010-020-03711-z
Narra, MR., Rajender, K., Reddy, RR., Murty, US., Begum, G.
2017. Insecticide induced stress response and recuperation
in fish: Biomarkers in blood and tissues related to
oxidative stress. Chemosphere, 168, 350–357. Doi:10.1016/j.
chemosphere.2016.10.066
Nilsson, GE., Dymowska, A., Stecyk, JA. 2012. New insights
into the plasticity of gill structure. Resp. Physiol. Neurobiol.,
184(3), 214-222. Doi:10.1016/j.resp.2012.07.012
Pałecz, D., Komuński, R., Gabryelak, T. 2005. Na+ K+-
ATPase activity as a biomarker of toxaphene toxicity in Unio
tumidus. Toxicology in vitro, 19(5), 707-712. Doi:10.1016/j.
tiv.2005.03.014
Parvez, S., Sayeed, I., Raisuddin, S. 2006. Decreased gill ATPase
activities in the freshwater fish Channa punctata (Bloch)
exposed to a diluted paper mill effluent. Ecotoxicol. Environ.
Saf., 65(1), 62-66. doi.org/10.1016/j.ecoenv.2005.07.010
Sattelle, DB., Cordova, D., Cheek, TR. 2008. Insect ryanodine
receptors: molecular targets for novel pest control chemicals.
Invert. Neurosci., 8(3), 107.
Stagg, RM., Shuttle Worth, TJ., 1982. The effects of copper on
ionic regulation by thegills of the seawater adapted flounder,
Platichys flesus L. J. Comp. Physiol., 149, 83–90.
Stahl, LL., Snyder, BD., Olsen, AR., Pitt, JL. 2009.
Contaminants in fish tissue from US lakes and reservoirs: a
national probabilistic study. Environ. Monit Assess, 150(1-4),
3-19. Doi:10.1007/s10661-008-0669-8
Suvetha, L., Ramesh, M., Saravanan, M. 2010. Influence of
cypermethrin toxicity on ionic regulation and gill Na+/K+-
ATPase activity of a freshwater teleost fish Cyprinus carpio.
Environ. toxicol. Pharmacol., 29(1), 44-49.
Temiz Ö., 2020. Experimental study on the evaluation of
ionoregulation enzymes, heat shock protein, DNA oxidation
and apoptosis in male mice heart tissue in exposure of
emamectin benzoate. EC Pharmacol. Toxicol., 8(3): 01-08.
Temiz, Ö., Cogun, H. Y., & Kargin, F. 2018. Influence of
chlorantraniliprole toxicity on ionic regulation of gill and
muscle ATPase activity of nile fish (Oreochromis niloticus).
Fresen. Environ. Bull, 27, 5027-5032.
Vieira, CED., dos Reis Martinez, CB. 2018. The pyrethroid
λ-cyhalothrin induces biochemical, genotoxic, and physiological
alterations in the teleost Prochilodus lineatus. Chemosphere, 210,
958-967. Doi:10.1016/j.chemosphere.2018.07.115
Vijayavel, K., Gopalakrishnan, S., Balasubramanian, MP.
2007. Sublethal effect of silver and chromium in the green
mussel Perna viridis with reference to alterations in oxygen
uptake, filtration rate and membrane bound ATPase system
as biomarkers. Chemosphere, 69(6), 979-986. Doi:10.1016/j.
chemosphere.2007.05.011
Webb, D., Gangnon, MM. Rose, T. 2005. Metabolic enzyme
activities in black bream, Acanthopagrus butcheri from the swan
canning estuary, Western Austrilia. Comp. Biochem. Physiol.
Part C 141,356-365. Doi:10.1016/j.cbpc.2005.07.010
Year 2021,
Volume: 11 Issue: 1, 23 - 27, 09.06.2021
Agrahari, S., Gopal, K. 2008. Inhibition of Na+–K+-ATPase in
different tissues of freshwater fish Channa punctatus (Bloch)
exposed to monocrotophos. Pestic. Biochem. Phys., 92(2), 57-60.
Doi:10.1016/j.pestbp.2008.06.003
Atkinson, A., Gatemby, A.O., Lowe, A.G. 1973. The
determination of inorganic orthophosphate in biological
systems. Biochim. Biophys. Acta., 320:195–204. Doi:
10.1016/0304-4165(73)90178-5
Blocksom, KA., Walters, DM., Jicha, TM., Lazorchak, JM.,
Angradi, TR., Bolgrien, DW. 2010. Persistent organic
pollutants in fish tissue in the mid-continental great rivers of
the United States. Sci. Total Environ., 408(5), 1180-1189. Doi:
10.1016/j.scitotenv.2009.11.040
Boyle, RT., Oliveira, LF., Bianchini, A., Souza, MM. 2013.
The Effects of Copper on Na+/K+-ATPase and Aquaporin
Expression in Two Euryhaline Invertebrates. Bull Environ.
Contam. Toxicol. 90:387–390. Doi:10.1007/s00128-012-0949-
4
Bradford, M. 1976. A rapid and sensitive method for the
quantitation of microgram quantities of protein utilizing the
principle of protein-dye binding. Anal. Biochem., 72:248-254.
Doi: 10.1006/abio.1976.9999
Ciacci, C., Barmo, C., Gallo, G., Maisano, M., Cappello,
T., D’Agata, A., Canesi, L. 2012. Effects of sublethal,
environmentally relevant concentrations of hexavalent
chromium in the gills of Mytilus galloprovincialis. Aqua.
Toxicol., 120, 109-118. Doi:10.1016/j.aquatox.2012.04.015
Davis, PW., Wedemeyer, GA., 1971. Na+/K+ activated ATPase
inhibition in rainbowtrout. A site for organochlorine
pesticide toxicity. Comp. Biochem. Physiol. 40,823–827.
Doi:10.1016/0305-0491(71)90157-X
Dellali, M., El Bour, N., Mahmoud, M., Patricia, A., Mahmoudi,
E. 2010. Ecolo. Indicators, 10,696.
Evans, DH., Piermarini, PM., Choe, KP. 2005. The
multifunctional fish gill: dominant site of gas exchange,
osmoregulation, acid-base regulation, and excretion of
nitrogenous waste. Physiol. Rev., 85(1), 97-177. Doi:10.1152/
physrev.00050.2003
Jorgensen, PL., Hakansson, KO. and Karlish, SJD. 2003.
Structure and mechanism of Na+/K+– ATPase: functional
sites and their interactions, Annu. Rev. Physiol., 65: 817-849.
Doi:10.1146/annurev.physiol.65.092101.142558
Lahm, GP., Selby, T. P., Freudenberger, JH., Stevenson, TM.,
Myers, BJ., Seburyamo, G., Cordova, D. 2005. Insecticidal
anthranilic diamides: a new class of potent ryanodine receptor
activators. Bioorg. Med. Chem. Lett., 15(22), 4898-4906.
Doi:10.1016/j.bmcl.2005.08.034
Lahm, GP., Cordova, D., Barry, JD. 2009. New and selective
ryanodine receptor activators for insect control. Bioorg. Med.
Chem., 17(12), 4127-4133. Doi:10.1016/j.bmc.2009.01.018
Mishra, D., Srivastav, SK., & Srivastav, AK. 2005. Effects
of the insecticide cypermethrin on plasma calcium and
ultimobranchial gland of a teleost, Heteropneustes fossilis.
Ecotoxicol. Environ. Saf., 60(2), 193-197. Doi:10.1016/j.
ecoenv.2003.12.020
Murali, M., Carvalho, MS., & Shivanandappa, T. 2020.
Oxidative stress-mediated cytotoxicity of Endosulfan is
causally linked to the inhibition of NADH dehydrogenase
and Na+, K+-ATPase in Ehrlich ascites tumor cells. Mol. Cell.
Biochem., 1-10. Doi:10.1007/s11010-020-03711-z
Narra, MR., Rajender, K., Reddy, RR., Murty, US., Begum, G.
2017. Insecticide induced stress response and recuperation
in fish: Biomarkers in blood and tissues related to
oxidative stress. Chemosphere, 168, 350–357. Doi:10.1016/j.
chemosphere.2016.10.066
Nilsson, GE., Dymowska, A., Stecyk, JA. 2012. New insights
into the plasticity of gill structure. Resp. Physiol. Neurobiol.,
184(3), 214-222. Doi:10.1016/j.resp.2012.07.012
Pałecz, D., Komuński, R., Gabryelak, T. 2005. Na+ K+-
ATPase activity as a biomarker of toxaphene toxicity in Unio
tumidus. Toxicology in vitro, 19(5), 707-712. Doi:10.1016/j.
tiv.2005.03.014
Parvez, S., Sayeed, I., Raisuddin, S. 2006. Decreased gill ATPase
activities in the freshwater fish Channa punctata (Bloch)
exposed to a diluted paper mill effluent. Ecotoxicol. Environ.
Saf., 65(1), 62-66. doi.org/10.1016/j.ecoenv.2005.07.010
Sattelle, DB., Cordova, D., Cheek, TR. 2008. Insect ryanodine
receptors: molecular targets for novel pest control chemicals.
Invert. Neurosci., 8(3), 107.
Stagg, RM., Shuttle Worth, TJ., 1982. The effects of copper on
ionic regulation by thegills of the seawater adapted flounder,
Platichys flesus L. J. Comp. Physiol., 149, 83–90.
Stahl, LL., Snyder, BD., Olsen, AR., Pitt, JL. 2009.
Contaminants in fish tissue from US lakes and reservoirs: a
national probabilistic study. Environ. Monit Assess, 150(1-4),
3-19. Doi:10.1007/s10661-008-0669-8
Suvetha, L., Ramesh, M., Saravanan, M. 2010. Influence of
cypermethrin toxicity on ionic regulation and gill Na+/K+-
ATPase activity of a freshwater teleost fish Cyprinus carpio.
Environ. toxicol. Pharmacol., 29(1), 44-49.
Temiz Ö., 2020. Experimental study on the evaluation of
ionoregulation enzymes, heat shock protein, DNA oxidation
and apoptosis in male mice heart tissue in exposure of
emamectin benzoate. EC Pharmacol. Toxicol., 8(3): 01-08.
Temiz, Ö., Cogun, H. Y., & Kargin, F. 2018. Influence of
chlorantraniliprole toxicity on ionic regulation of gill and
muscle ATPase activity of nile fish (Oreochromis niloticus).
Fresen. Environ. Bull, 27, 5027-5032.
Vieira, CED., dos Reis Martinez, CB. 2018. The pyrethroid
λ-cyhalothrin induces biochemical, genotoxic, and physiological
alterations in the teleost Prochilodus lineatus. Chemosphere, 210,
958-967. Doi:10.1016/j.chemosphere.2018.07.115
Vijayavel, K., Gopalakrishnan, S., Balasubramanian, MP.
2007. Sublethal effect of silver and chromium in the green
mussel Perna viridis with reference to alterations in oxygen
uptake, filtration rate and membrane bound ATPase system
as biomarkers. Chemosphere, 69(6), 979-986. Doi:10.1016/j.
chemosphere.2007.05.011
Webb, D., Gangnon, MM. Rose, T. 2005. Metabolic enzyme
activities in black bream, Acanthopagrus butcheri from the swan
canning estuary, Western Austrilia. Comp. Biochem. Physiol.
Part C 141,356-365. Doi:10.1016/j.cbpc.2005.07.010
Temiz Ö, Aytekin T, Çoğun HY, Kargın F (June 1, 2021) Klorantraniliprol İndüklü Toksisitenin Oreochromis niloticus Solungaç İyon Taşınım Enzim Aktivitelerine Etkileri. Karaelmas Fen ve Mühendislik Dergisi 11 1 23–27.
IEEE
Ö. Temiz, T. Aytekin, H. Y. Çoğun, and F. Kargın, “Klorantraniliprol İndüklü Toksisitenin Oreochromis niloticus Solungaç İyon Taşınım Enzim Aktivitelerine Etkileri”, Karaelmas Fen ve Mühendislik Dergisi, vol. 11, no. 1, pp. 23–27, 2021.
ISNAD
Temiz, Özge et al. “Klorantraniliprol İndüklü Toksisitenin Oreochromis Niloticus Solungaç İyon Taşınım Enzim Aktivitelerine Etkileri”. Karaelmas Fen ve Mühendislik Dergisi 11/1 (June 2021), 23-27.
JAMA
Temiz Ö, Aytekin T, Çoğun HY, Kargın F. Klorantraniliprol İndüklü Toksisitenin Oreochromis niloticus Solungaç İyon Taşınım Enzim Aktivitelerine Etkileri. Karaelmas Fen ve Mühendislik Dergisi. 2021;11:23–27.
MLA
Temiz, Özge et al. “Klorantraniliprol İndüklü Toksisitenin Oreochromis Niloticus Solungaç İyon Taşınım Enzim Aktivitelerine Etkileri”. Karaelmas Fen Ve Mühendislik Dergisi, vol. 11, no. 1, 2021, pp. 23-27.
Vancouver
Temiz Ö, Aytekin T, Çoğun HY, Kargın F. Klorantraniliprol İndüklü Toksisitenin Oreochromis niloticus Solungaç İyon Taşınım Enzim Aktivitelerine Etkileri. Karaelmas Fen ve Mühendislik Dergisi. 2021;11(1):23-7.