Determination of Total Phenol Contents, Antibacterial and Antioxidant Activity of Some Mosses Species

Year 2022, Volume: 12 Issue: 1, 86 - 92, 01.06.2022

Şükran Öztürk Yasin Hazer Banu Kaşkatepe Muhammet Ören

Abstract

The aim of this study were to determine total phenol contents, antibacterial and antioxidant activity of bryophyte species including
Plasteurhynchium striatum (Spruce) M. Fleisch, Palamocladium euchloron (Bruch ex Müll. Hal.) Wijk & Margad. and Cratoneuron filicinum
(Hedw.) Spruce and Campyliadelphus chrysophylus (Brid.) R.S. Chopra. Antibacterial activity of methanolic, ethanolic, chloroform,
acetone and water extracts of these species was determined by microdilution method. Antioxidant activity of these species was
evaluated by 2,2-diphenyl-1-picrylhydrazyl free radical-scavenging ability. The total phenol content of these species was determined
quantitatively using the folin ciocalteu reagent, with gallic acid equivalents as the standard.
The highest DPPH free radical scavenging activity was observed in about 65% C. filicinum. Total phenolic content of P. euchloron
was determined the highest extract value of 0,027±0,002 mg/g. The methanol extract of bryophytes was found to have significant
antioxidant activity and phenolic contents. Antibacterial effect of the extracts was not observed against the tested microorganisms

Keywords

Antimicrobial activity, Antioxidant activity, Bryophytes, Total phenol contents

Bazı Yosun Türlerinin Toplam Fenol İçeriklerinin, Antibakteriyel ve Antioksidan Aktivitelerinin Belirlenmesi

Abstract

Bu çalışmanın amacı Cratoneuron filicinum (Hedw.) Ladin, Campyliadelphus chrysophylus (Brid.) R.S. Chopra. Plasteurhynchium striatum
(Spruce) M. Fleisch, Palamocladium euchloron (Bruch ex Müll. Hal.) Wijk & Margad gibi briyofit türlerinin toplam fenol içeriklerini,
antibakteriyel ve antioksidan aktivitelerini belirlemektir. Bu türlerin metanolik, etanolik, kloroform, aseton ve su ekstraktlarının
antibakteriyel aktiviteleri mikrodilüsyon yöntemi ile, antioksidan aktivitesi 2,2-difenil-1-pikrilhidrazil serbest radikal süpürme yeteneği
ile, Toplam fenol içerikleri ise standart olarak gallik asit eşdeğerleri ile folin ciocalteu reaktifi kullanılarak nicel olarak belirlenmiştir.
En yüksek DPPH serbest radikal süpürme aktivitesi yaklaşık %65 ile C. filicinum’da gözlendi. P. euchloron’un toplam fenolik içeriği
en yüksek ekstre değeri 0,027±0,002 mg/g olarak belirlenmiştir. Briyofitlerin metanol ekstraktının önemli antioksidan aktiviteye ve
fenolik içeriğe sahip olduğu bulundu. Ekstraktların test edilen mikroorganizmalara karşı antibakteriyel etkisi gözlenmedi.

Keywords

Antimikrobiyal aktivite, Antioksidan aktivite, Briyofitler, Toplam fenol içeriği

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