Araştırma Makalesi
BibTex RIS Kaynak Göster

Callinectes sapidus’un (Mavi Yengeç) Hepatopankreas Esteraz (E. C. 3.1.1.1) Aktivitesine Metal İyonlarının (Zn+2, Co+2, Cd+2, Ni+2) in Vitro Etkilerinin Belirlenmesi

Yıl 2022, Cilt: 12 Sayı: 1, 32 - 37, 01.06.2022

Salih Görgün

Öz

Bu çalışmada insanlar tarafından besin olarak tüketilen bir yengeç türü olan Callinectes sapidus (Rathbun, 1896)’nın hepatopankreas
esteraz (E. C. 3.1.1.1) aktivitesine çinko (Zn), kobalt (Co), kadmiyum (Cd), nikel (Ni) metallerinin etkileri in vitro olarak belirlenmiştir.
Elde edilen sonuçlara göre; araştırılan tüm metal iyonlarının hepatopankreas esteraz aktivitesi üzerine inhibisyon etkisi sergilemekle
birlikte, IC50 değerlerinin 0.83 mM (Cd) ile 32.21 mM (Co) arasında değişim gösterdiği bulunmuştur. Metal iyonlarının inhibisyon
tipleri değerlendirildiğinde, Zn ve Co un-kompetitif inhibisyona neden olurken, Cd ve Ni karışık tip inhibisyon sergilemişlerdir. Elde
edilen veriler, çevresel metal kirliliğinin belirlenmesinde mavi yengecin rolüne biyokimyasal veriler sunmaktadır.

Anahtar Kelimeler

Callinectes sapidus hepatopankreas esteraz metal iyonları

Destekleyen Kurum

Sivas Cumhuriyet Üniversitesi

Proje Numarası

F-624

Teşekkür

Bu çalışma Sivas Cumhuriyet Üniversitesi Bilimsel Araştırma Projeleri (CÜBAP) Birimi tarafından F-624 No’lu proje olarak desteklenmiştir. Bu nedenle, teşekkürlerimizi sunuyoruz.

Kaynakça

  • Althaljı, M , Görgün, S. 2019. Purification and characterization of an esterase from larval Diplolepis fructuum (Rübsaamen, 1895) (Hymenoptera: Cynipidae). Turk. Entomol. Derg.-Tu. 43(4): 367-376. Doi: 10.16970/entoted.533752
  • Arya, S., Trivedi, JN., Vachhrajani, KD. 2014. Brachyuran crabs as a biomonitoring tool: A conceptual framework for chemical pollution assessment. Int. Res. J. of Environment Sci. 3(1): 49-57
  • Cheng, L., Chen, Y., Zheng, YY., Zhan, Y., Zhao, H., Zhou, JL. 2017. Bioaccumulation of sulfadiazine and subsequent enzymatic activities in Chinese mitten crab (Eriocheir sinensis). Mar. Pollut. Bullet. 121: 176-182. Doi: 10.1016/j.marpolbul.2017.06.006
  • Cheng, L., Zhou, JL., Cheng, J. 2018. Bioaccumulation, tissue distribution and joint toxicity of erythromycin and cadmium in Chinese mitten crab (Eriocheir sinensis). Chemosphere. 210: 267-278. Doi: 10.1016/j.chemosphere.2018.07.005
  • Chou, C., Paon, L., Moffatt, J. 2002. Cadmium, copper, manganese, silver, and zinc in rock crab (Cancer irroratus) from highly copper contaminated sites in the inner Bay of Fundy, Atlantic Canada. Bull. Environ. Contam. Toxicol. 68: 885–892. Doi: 10.1007/s00128-002-0037-2
  • Cresswell, T., Simpson, SL., Mazumder, D., Callaghan, PD., Nguyen AP. 2015. Bioaccumulation kinetics and organ distribution of cadmium and zinc in the freshwater decapod crustacean Macrobrachium australiense. Environ. Sci. Technol. 49(2): 1182-1189. Doi: 10.1021/es505254w
  • Çoğun, HY., Firat, Ö., Aytekin, T., Firidin, G., Firat, Ö., Varkal, H., Temiz, Ö., Kargin, F. 2017. Heavy metals in the blue crab (Callinectes sapidus) in Mersin Bay, Turkey. Bull. Environ. Contam. Toxicol. 98: 824–829. Doi: 10.1007/s00128-017-2086-6
  • Elumalai, M., Antunes, C., Guilhermino, L. 2005. Alterations of reproductive parameters in the crab Carcinus maenas after exposure to metals. Water Air Soil Pollut. 160: 245–258. Doi: 10.1007/s11270-005-2992-9
  • Franceschini-Vicentini, IB., Ribeiro, K., Papa, LP. 2009. Histoarchitectural features of the hepatopancreas of the amazon river prawn Macrobrachium amazonicum. Int. J. Morphol. 27: 121-128. Doi: 10.4067/S0717-95022009000100022
  • Frasco, MF., Erzen, I., Stojan, J., Guilhermino, L. 2010. Localization and properties of cholinesterases in the common prawn (Palaemon serratus): A kinetic-histochemical study. Biol. Bull. 218: 1-5. Doi: 10.1086/BBLv218n1p1
  • Lima, AVB., Guerra AL., de Almeida, EA., Taddei, FG., Castiglioni, L. 2013. Characterization of esterase patterns in hepatopancreas of three species of Macrobrachium (Palaemonidae). Biochem. Syst. Ecol. 47: 132-138. Doi: 10.1016/j.bse.2012.10.006
  • Liu, J., Wu, H., Feng, J., Li, Z., Lin, G. 2014. Heavy metal contamination and ecological risk assessments in sediments and zoobenthos of selected mangrove ecosystems, South China. Catena. 119, 136–142. Doi: 10.1016/j.catena.2014.02.009
  • Peterson, MJ., Smith, JG., Southworth, GR., Ryon, MG., Eddlemon, GK. 2002. Trace element contamination in benthic macroinvertebrates from a small stream near a uranium mill Tailings Site. Environ. Monit. Assess. 74:193–208. doi:10.1023/A:1013872103460
  • Rainbow, PS., Luoma, SN. 2011. Metal toxicity, uptake and bioaccumulation in aquatic invertebrates: Modelling zinc in crustaceans. Aquat. Toxicol. 105(3−4): 455−465. Doi: 10.1016/j.aquatox.2011.08.001
  • Soegianto, A., Winarni, D., Handayani, US., Hartati, H. 2013. Bioaccumulation, elimination, and toxic effect of cadmium on structure of gills and hepatopancreas of freshwater prawn Macrobrachium sintangese (De Man, 1898). Water Air Soil. Pollut. 224: 1575-1585. Doi: 10.1007/s11270-013-1575-4
  • Stubblefield, WA., Genderen, EV, Cardwell, AS., Heijerick, DG., Janssen, CR., De Schamphelaere, KAC. 2020. Acute and chronic toxicity of cobalt to freshwater organisms: using a species sensitivity distribution approach to establish international water quality standards. Environ. Toxicol. Chem. 39(4): 799-811. Doi: 10.1002/etc.4662
  • Turoczy, NJ., Mitchell, BD., Levings, AH., Rajendram, VS. 2001. Cadmium, copper, mercury, and zinc concentrations in tissues of the king crab (Pseudocarcinus gigas) from southeast Australian waters. Environ. Int. 27(4): 327-34. Doi: 10.1016/s0160-4120(01)00064-2
  • Türkmen, A., Türkmen, M., Tepe, Y., Mazlum, Y., Oymael, S. 2006. Metal concentrations in blue crab (Callinectes sapidus) and mullet (Mugil cephalus) in İskenderun Bay, Northern East Mediterranean, Turkey. Bull. Environ. Contam. Toxicol. 77:186–193. Doi: 10.1007/s00128-006-1049-0
  • Verma, RS. 2012. Acute toxicity of nickel to fresh water prawns. Turk. J. Zool. 36(4): 534-542. Doi: 10.3906/zoo-1102-18
  • Zhang, Z. Fang, Z., Li, J., Sui, T., Lin, L., Xu, X. 2019. Copper, zinc, manganese, cadmium and chromium in crabs from the mangrove wetlands in Qi'ao Island, South China: Levels, bioaccumulation and dietary exposure. WEE. 1(2019): 26-32. Doi: 10.1016/j.wsee.2019.09.001
  • Zhao, S., Feng, C., Quan, W., Chen, X., Niu, J., Shen, Z. 2012. Role of living environments in the accumulation characteristics of heavy metals in fishes and crabs in the Yangtze River Estuary, China. Mar. Pollut. Bullet. 64, 1163-1171. Doi: 10.1016/j.marpolbul.2012.03.023

Determination of in Vitro Effects of Metal Ions (Zn+2, Co+2, Cd+2, Ni+2) on the Hepatopancreas Esterase (E. C. 3.1.1.1) Activity of Callinectes sapidus (Blue Crab)

Yıl 2022, Cilt: 12 Sayı: 1, 32 - 37, 01.06.2022

Salih Görgün

Öz

In this study, in vitro effects of zinc (Zn), cobalt (Co), cadmium (Cd), nickel (Ni) on the hepatopancreas esterase (E. C. 3.1.1.1)
activity of Callinectes Sapidus (Rathbun, 1896), a crab species consumed as a food by humans, were investigated. According to the
obtained results; IC50 values were found to vary between 0.83 mM (Cd) and 32.21 mM (Co), in addition to the inhibition effect of all
the metal ions investigated on the hepatopancreas esterase activity. When assessed the inhibition types of the metal ions investigated,
while Zn and Co caused un-competitive inhibition, Cd and Ni had exhibited mixed type inhibition. The data obtained provide
biochemical data on the role of blue crab in determination of environmental metal pollution.

Anahtar Kelimeler

Callinectes sapidus Hepatopancreas Esterase Metal ions

Proje Numarası

F-624

Kaynakça

  • Althaljı, M , Görgün, S. 2019. Purification and characterization of an esterase from larval Diplolepis fructuum (Rübsaamen, 1895) (Hymenoptera: Cynipidae). Turk. Entomol. Derg.-Tu. 43(4): 367-376. Doi: 10.16970/entoted.533752
  • Arya, S., Trivedi, JN., Vachhrajani, KD. 2014. Brachyuran crabs as a biomonitoring tool: A conceptual framework for chemical pollution assessment. Int. Res. J. of Environment Sci. 3(1): 49-57
  • Cheng, L., Chen, Y., Zheng, YY., Zhan, Y., Zhao, H., Zhou, JL. 2017. Bioaccumulation of sulfadiazine and subsequent enzymatic activities in Chinese mitten crab (Eriocheir sinensis). Mar. Pollut. Bullet. 121: 176-182. Doi: 10.1016/j.marpolbul.2017.06.006
  • Cheng, L., Zhou, JL., Cheng, J. 2018. Bioaccumulation, tissue distribution and joint toxicity of erythromycin and cadmium in Chinese mitten crab (Eriocheir sinensis). Chemosphere. 210: 267-278. Doi: 10.1016/j.chemosphere.2018.07.005
  • Chou, C., Paon, L., Moffatt, J. 2002. Cadmium, copper, manganese, silver, and zinc in rock crab (Cancer irroratus) from highly copper contaminated sites in the inner Bay of Fundy, Atlantic Canada. Bull. Environ. Contam. Toxicol. 68: 885–892. Doi: 10.1007/s00128-002-0037-2
  • Cresswell, T., Simpson, SL., Mazumder, D., Callaghan, PD., Nguyen AP. 2015. Bioaccumulation kinetics and organ distribution of cadmium and zinc in the freshwater decapod crustacean Macrobrachium australiense. Environ. Sci. Technol. 49(2): 1182-1189. Doi: 10.1021/es505254w
  • Çoğun, HY., Firat, Ö., Aytekin, T., Firidin, G., Firat, Ö., Varkal, H., Temiz, Ö., Kargin, F. 2017. Heavy metals in the blue crab (Callinectes sapidus) in Mersin Bay, Turkey. Bull. Environ. Contam. Toxicol. 98: 824–829. Doi: 10.1007/s00128-017-2086-6
  • Elumalai, M., Antunes, C., Guilhermino, L. 2005. Alterations of reproductive parameters in the crab Carcinus maenas after exposure to metals. Water Air Soil Pollut. 160: 245–258. Doi: 10.1007/s11270-005-2992-9
  • Franceschini-Vicentini, IB., Ribeiro, K., Papa, LP. 2009. Histoarchitectural features of the hepatopancreas of the amazon river prawn Macrobrachium amazonicum. Int. J. Morphol. 27: 121-128. Doi: 10.4067/S0717-95022009000100022
  • Frasco, MF., Erzen, I., Stojan, J., Guilhermino, L. 2010. Localization and properties of cholinesterases in the common prawn (Palaemon serratus): A kinetic-histochemical study. Biol. Bull. 218: 1-5. Doi: 10.1086/BBLv218n1p1
  • Lima, AVB., Guerra AL., de Almeida, EA., Taddei, FG., Castiglioni, L. 2013. Characterization of esterase patterns in hepatopancreas of three species of Macrobrachium (Palaemonidae). Biochem. Syst. Ecol. 47: 132-138. Doi: 10.1016/j.bse.2012.10.006
  • Liu, J., Wu, H., Feng, J., Li, Z., Lin, G. 2014. Heavy metal contamination and ecological risk assessments in sediments and zoobenthos of selected mangrove ecosystems, South China. Catena. 119, 136–142. Doi: 10.1016/j.catena.2014.02.009
  • Peterson, MJ., Smith, JG., Southworth, GR., Ryon, MG., Eddlemon, GK. 2002. Trace element contamination in benthic macroinvertebrates from a small stream near a uranium mill Tailings Site. Environ. Monit. Assess. 74:193–208. doi:10.1023/A:1013872103460
  • Rainbow, PS., Luoma, SN. 2011. Metal toxicity, uptake and bioaccumulation in aquatic invertebrates: Modelling zinc in crustaceans. Aquat. Toxicol. 105(3−4): 455−465. Doi: 10.1016/j.aquatox.2011.08.001
  • Soegianto, A., Winarni, D., Handayani, US., Hartati, H. 2013. Bioaccumulation, elimination, and toxic effect of cadmium on structure of gills and hepatopancreas of freshwater prawn Macrobrachium sintangese (De Man, 1898). Water Air Soil. Pollut. 224: 1575-1585. Doi: 10.1007/s11270-013-1575-4
  • Stubblefield, WA., Genderen, EV, Cardwell, AS., Heijerick, DG., Janssen, CR., De Schamphelaere, KAC. 2020. Acute and chronic toxicity of cobalt to freshwater organisms: using a species sensitivity distribution approach to establish international water quality standards. Environ. Toxicol. Chem. 39(4): 799-811. Doi: 10.1002/etc.4662
  • Turoczy, NJ., Mitchell, BD., Levings, AH., Rajendram, VS. 2001. Cadmium, copper, mercury, and zinc concentrations in tissues of the king crab (Pseudocarcinus gigas) from southeast Australian waters. Environ. Int. 27(4): 327-34. Doi: 10.1016/s0160-4120(01)00064-2
  • Türkmen, A., Türkmen, M., Tepe, Y., Mazlum, Y., Oymael, S. 2006. Metal concentrations in blue crab (Callinectes sapidus) and mullet (Mugil cephalus) in İskenderun Bay, Northern East Mediterranean, Turkey. Bull. Environ. Contam. Toxicol. 77:186–193. Doi: 10.1007/s00128-006-1049-0
  • Verma, RS. 2012. Acute toxicity of nickel to fresh water prawns. Turk. J. Zool. 36(4): 534-542. Doi: 10.3906/zoo-1102-18
  • Zhang, Z. Fang, Z., Li, J., Sui, T., Lin, L., Xu, X. 2019. Copper, zinc, manganese, cadmium and chromium in crabs from the mangrove wetlands in Qi'ao Island, South China: Levels, bioaccumulation and dietary exposure. WEE. 1(2019): 26-32. Doi: 10.1016/j.wsee.2019.09.001
  • Zhao, S., Feng, C., Quan, W., Chen, X., Niu, J., Shen, Z. 2012. Role of living environments in the accumulation characteristics of heavy metals in fishes and crabs in the Yangtze River Estuary, China. Mar. Pollut. Bullet. 64, 1163-1171. Doi: 10.1016/j.marpolbul.2012.03.023
Toplam 21 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Mühendislik
Bölüm Araştırma Makaleleri
Yazarlar

Salih Görgün 0000-0002-2121-2577

Proje Numarası F-624
Yayımlanma Tarihi 1 Haziran 2022
Yayımlandığı Sayı Yıl 2022 Cilt: 12 Sayı: 1

Kaynak Göster

APA Görgün, S. (2022). Callinectes sapidus’un (Mavi Yengeç) Hepatopankreas Esteraz (E. C. 3.1.1.1) Aktivitesine Metal İyonlarının (Zn+2, Co+2, Cd+2, Ni+2) in Vitro Etkilerinin Belirlenmesi. Karaelmas Fen Ve Mühendislik Dergisi, 12(1), 32-37. https://doi.org/10.7212/karaelmasfen.960789
AMA Görgün S. Callinectes sapidus’un (Mavi Yengeç) Hepatopankreas Esteraz (E. C. 3.1.1.1) Aktivitesine Metal İyonlarının (Zn+2, Co+2, Cd+2, Ni+2) in Vitro Etkilerinin Belirlenmesi. Karaelmas Fen ve Mühendislik Dergisi. Haziran 2022;12(1):32-37. doi:10.7212/karaelmasfen.960789
Chicago Görgün, Salih. “Callinectes sapidus’un (Mavi Yengeç) Hepatopankreas Esteraz (E. C. 3.1.1.1) Aktivitesine Metal İyonlarının (Zn+2, Co+2, Cd+2, Ni+2) in Vitro Etkilerinin Belirlenmesi”. Karaelmas Fen Ve Mühendislik Dergisi 12, sy. 1 (Haziran 2022): 32-37. https://doi.org/10.7212/karaelmasfen.960789.
EndNote Görgün S (01 Haziran 2022) Callinectes sapidus’un (Mavi Yengeç) Hepatopankreas Esteraz (E. C. 3.1.1.1) Aktivitesine Metal İyonlarının (Zn+2, Co+2, Cd+2, Ni+2) in Vitro Etkilerinin Belirlenmesi. Karaelmas Fen ve Mühendislik Dergisi 12 1 32–37.
IEEE S. Görgün, “Callinectes sapidus’un (Mavi Yengeç) Hepatopankreas Esteraz (E. C. 3.1.1.1) Aktivitesine Metal İyonlarının (Zn+2, Co+2, Cd+2, Ni+2) in Vitro Etkilerinin Belirlenmesi”, Karaelmas Fen ve Mühendislik Dergisi, c. 12, sy. 1, ss. 32–37, 2022, doi: 10.7212/karaelmasfen.960789.
ISNAD Görgün, Salih. “Callinectes sapidus’un (Mavi Yengeç) Hepatopankreas Esteraz (E. C. 3.1.1.1) Aktivitesine Metal İyonlarının (Zn+2, Co+2, Cd+2, Ni+2) in Vitro Etkilerinin Belirlenmesi”. Karaelmas Fen ve Mühendislik Dergisi 12/1 (Haziran 2022), 32-37. https://doi.org/10.7212/karaelmasfen.960789.
JAMA Görgün S. Callinectes sapidus’un (Mavi Yengeç) Hepatopankreas Esteraz (E. C. 3.1.1.1) Aktivitesine Metal İyonlarının (Zn+2, Co+2, Cd+2, Ni+2) in Vitro Etkilerinin Belirlenmesi. Karaelmas Fen ve Mühendislik Dergisi. 2022;12:32–37.
MLA Görgün, Salih. “Callinectes sapidus’un (Mavi Yengeç) Hepatopankreas Esteraz (E. C. 3.1.1.1) Aktivitesine Metal İyonlarının (Zn+2, Co+2, Cd+2, Ni+2) in Vitro Etkilerinin Belirlenmesi”. Karaelmas Fen Ve Mühendislik Dergisi, c. 12, sy. 1, 2022, ss. 32-37, doi:10.7212/karaelmasfen.960789.
Vancouver Görgün S. Callinectes sapidus’un (Mavi Yengeç) Hepatopankreas Esteraz (E. C. 3.1.1.1) Aktivitesine Metal İyonlarının (Zn+2, Co+2, Cd+2, Ni+2) in Vitro Etkilerinin Belirlenmesi. Karaelmas Fen ve Mühendislik Dergisi. 2022;12(1):32-7.
 Kapak Resmi İndir