Development of a Digital Imaging-Based Colorimetric Analysis Method for the Determination of Phenolphthalein in Drinking Water

Yıl 2024, Cilt: 14 Sayı: 1, 70 - 79, 29.04.2024

Nihal Yücel , Ozan Yağmuroğlu

Öz

In this study, a digital imaging box-based colorimetric analysis method was developed for the determination of phenolphthalein in drinking water samples. In digital imaging, a smartphone camera was used as a sensor, and an application called "Color Detector" was used as software. Firstly, an optimization study was carried out for the variables that were considered to be effective on the analysis method. For this purpose, the distance of the sample from the phone lens, the location on the sample to be measured, the scale at the measurement location and pH values were optimized. Then, analysis was carried out with samples with different concentrations between 0.5-5.0 ppm. Linearity between 1-4 mg/L was observed in the analysis results. As a result of the calculations made to determine the method performance, the limit of detection (LOD) was found to be 0.58 mg/L and the percent relative standard deviation was 0.28% (n = 8). A recovery study was carried out on a drinking water sample to evaluate the performance of the method we developed in the real sample environment. As a result of the analysis, percentage recovery values were found to be in the range of 99%-106%. These values show that our method has high performance in drinking water samples.

Anahtar Kelimeler

Phenolphthalein, Colorimetry, Digital imaging, Drinking water

Proje Numarası

1919B012201439

İçme Sularında Fenolftalein Tayinine Yönelik Dijital Görüntüleme Temelli Kolorimetrik Analiz Yöntemi Geliştirilmesi

Öz

Bu çalışmada, fenolftaleinin içme suyu örneklerinde tayinine yönelik dijital görüntüleme kutusu temelli kolorimetrik analiz yöntemi geliştirilmiştir. Dijital görüntülemede algılayıcı olarak akıllı telefon kamerası kullanılmış, yazılım olarak ise "Color Detector" isimli uygulamadan yararlanılmıştır. İlk olarak analiz yöntemi üzerinde etkili olacağı değerlendirilen değişkenlere yönelik optimizasyon çalışması yapılmıştır. Bu amaçla, numunenin telefon lensine uzaklığı, numune üzerinde ölçüm alınacak konum, ölçüm konumundaki ölçek ve pH değerleri optimize edilmiştir. Daha sonra 0.5-5.0 ppm arasında farklı derişimlere sahip örneklerle analiz yapılmıştır. Analiz sonuçlarında 1-4 mg/L arasında lineerlik gözlenmiştir. Yöntem performansının belirlenmesine yönelik yapılan hesaplamalar sonucu tayin limiti (LOD) 0.58 mg/L ve yüzde bağıl standart sapma %0,28 (n=8) olarak bulunmuştur. Geliştirdiğimiz yöntemin gerçek numune ortamındaki performansının değerlendirilmesi amacıyla içme suyu örneğinde geri kazanım çalışması gerçekleştirilmiştir. Analizler sonucunda yüzde geri kazanım değerleri %99-%106 aralığında bulunmuştur. Bu değerler yöntemimizin içme suyu örneklerinde yüksek performans gösterdiğini ortaya koymaktadır.

Anahtar Kelimeler

Fenolftalein, Kolorimetri, Dijital görüntüleme, İçme suyu

Destekleyen Kurum

TÜBİTAK 2209-A Üniversite Öğrencileri Araştırma Projeleri Destekleme Programı

Proje Numarası

1919B012201439

Teşekkür

Çalışmamız TÜBİTAK 2209-A Üniversite Öğrencileri Araştırma Projeleri Destekleme Programı kapsamında desteklenmiştir.

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