Yeni Bir Schiff Bazı ve Platin Kompleksi: Amiloid Agregasyonunun İnhibisyonu

Yıl 2024, Cilt: 14 Sayı: 3, 1 - 11, 25.11.2024

Özge Özcan , Salih Günnaz , Sevil İrişli

Öz

Bu çalışma, amine ve imin donör atomlarını içeren bir Schiff bazı (L) bileşiğinin sentezini ve karakterizasyonunu incelemektedir. Özellikle, N1-(4-(benziloksi)benziliden)-N2-feniletan-1,2-diamin bileşiği ve onun Platin(II) kompleksi (Pt-L) üzerinde durulmaktadır. Hem ligandın hem de kompleksin yapısal aydınlatılması, Fourier-dönüşümlü kızılötesi spektroskopi (FT-IR), proton nükleer manyetik rezonans (1H-NMR), karbon-13 nükleer manyetik rezonans (13C-NMR) ve elementel analiz gibi çeşitli spektroskopik teknikler kullanılarak gerçekleştirilmiştir. Ayrıca, Pt-L kompleksinin Aβ1-42 agregasyonu üzerindeki potansiyel inhibisyon etkileri, insan nöroblastoma hücre hattı (SH-SY5Y) kullanılarak bir model sistem olarak araştırılmıştır. Pt-L kompleksinin SH-SY5Y nöroblastoma hücreleri üzerindeki sitotoksisitesi de incelenmiş ve IC50 değeri 19.22 μM olarak belirlenmiştir. Aβ agregasyonunun inhibisyon kinetiği ayrıca Thioflavin-T kullanılarak florometrik olarak incelenmiştir. Eğrinin altında kalan alanın değerlendirilmesi ile kompleksin 1.0:1.0 mol oranında amyloid ile %65 oranında etkileştiğini göstermiştir. Çalışmamızda elde edilen bu sonuçlar, kompleksin Aβ1-42. inhibitörü olarak umut verici bir bileşik olduğunu göstermektedir.

Anahtar Kelimeler

Anti-Alzheimer aktivitesi, sitotoksisite, schiff bazları, platin-schiff bazı kompleksleri

Etik Beyan

There are no ethical issues regarding the publication of this study

Destekleyen Kurum

Ege University

Proje Numarası

FYL -2019 21012

Teşekkür

Financial support from Ege University (Project FYL-2020-21432) is gratefully acknowledged

New Schiff Base and Its Platinum Complex: Inhibition of Amyloid Aggregation

Öz

This study investigates the synthesis and characterization of a Schiff base (L) comprising amine and imine donor atoms, specifically N1-(4-(benzyloxy)benzylidene)-N2-phenylethane-1,2-diamine, along with its Platinum(II) complex (Pt-L). The structural elucidation of both the ligand and complex is accomplished through various spectroscopic techniques including Fourier-transform infrared spectroscopy (FT-IR), proton nuclear magnetic resonance (1H-NMR), carbon-13 nuclear magnetic resonance (13C-NMR), and elemental analysis. Furthermore, the potential inhibitory effects of the Pt-L complex on Aβ1-42 aggregation are explored using the human neuroblastoma cell line (SH-SY5Y) as a model system. The cytotoxicity of the Pt-L complex on SH-SY5Y neuroblastoma cells is examined, revealing an IC50 value of 19.22 μM. The inhibition kinetics of Aβ aggregation are also investigated fluorometrically using Thioflavine-T. By analyzing the area under the curve, it is calculated that the complex (1.0:1.0 molar ratio) interacts with amyloid at a rate of 65%. These results obtained in our study show that the complex is promising in terms of inhibition of Aβ1-42.

Anahtar Kelimeler

Anti-Alzheimer activity, cytotoxicity, schiff base, platinum-schiff base complexes

Proje Numarası

FYL -2019 21012

Kaynakça

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