Chemical composition and antimicrobial activity of Myristica fragrans & Elettaria cardamomum essential oil

Year 2018, , 225 - 229, 15.09.2018

Osman Emre Özkan , Çağrı Olgun , Betül Güney Mahmut Gür , Kerim Güney , Saim Ateş

https://doi.org/10.17475/kastorman.356765

Cited By: 10

Abstract

Aim of study: The objectives in this study were to determine the chemical composition and antimicrobial activity of the essential oils from nutmeg (Myristica fragrans) and cardamom (Elettaria cardamomum).

Material and Methods: The essential oil was obtained from nutmeg and cardamom seeds through hydrodistillation method. The chemical composition of nutmeg and cardamom essential oil was analyzed by GC-MS. The antimicrobial activity of essential oil was investigated against Bacillus subtilis DSMZ 1971, Candida albicans DSMZ 1386, Enterococcus faecalis ATCC 29212, Enterobacter aerogenes ATCC 13048, Enterococcus durans, Enterococcus faecium, Escherichia coli ATCC 25922, Klebsiella pneumoniae, Listeria monocytogenes, Listeria innocua, Pseudomonas aeruginosa DSMZ 50071, Pseudomonas fluorescence P1, Salmonella enteritidis ATCC 13076, Salmonella infantis, Salmonella kentucky, Salmonella typhimurium SL 1344, Staphylococcus aureus ATCC 25923 and Staphylococcus epidermidis DSMZ 20044 with minimum inhibitory concentration (MIC) method.

Main results: GC-MS analyzes shows that γ-terpinene (19.16%), (-)-terpinen-4-ol (14.08%), α-pinene (6.75%), myristicin (6.18%), β-pinene (4.85%), sylvestrene (4.72%), elemicin (4.68%) and safrole (4.17%) identified in nutmeg essential oil. Also, α-terpinyl acetate (34.55%), eucalyptol (24.91%), (-)-terpinen-4-ol (10.63%), linalool (7.69%) and neryl butyrate (6.58%) compounds identified in cardamom essential oil as major compounds. Nutmeg and cardamom essential oil showed an antimicrobial activity against almost all test microorganisms.

Research highlights: This study suggests that nutmeg and cardamom essential oil may be useful in the medicinal application and food industry as a preservative.

Keywords

Nutmeg, Cardamom, Essential oil, Antimicrobial activity

Myristica fragrans ve Elettaria cardamomum uçucu yağlarının kimyasal bileşimi ve anti mikrobiyal aktivitesi

Abstract

Çalışmanın amacı: Bu çalışmanın amacı, muskat (Myristica fragrans) ve kakule (Elettaria cardamomum) ‘den elde edilen uçucu yağlarının kimyasal içeriğinin ve antimikrobiyal aktivite özelliklerinin tespit edilmesidir.

Materyal ve Yöntem: Muskat ve kakule tohumlarından uçucu yağ eldesi hidro distilasyon yöntemi ile yapılmıştır. Söz konusu bu uçucu yağların kimyasal içeriği GC-MS cihazı ile analiz edilmiştir. Anti mikrobiyal aktivite deneyi Bacillus subtilis DSMZ 1971, Candida albicans, Enterococcus faecalis ATCC 29212, Enterobacter aerogenes ATCC 13048, Enterococcus durans, Enterococcus faecium, Escherichia coli ATCC 25922, Klebsiella pneumoniae, Listeria monocytogenes, Listeria innocua, Pseudomonas aeruginosa DSMZ 50071, Pseudomonas fluorescence P1, Salmonella enteritidis ATCC 13076, Salmonella infantis, Salmonella kentucky, Salmonella typhimurium SL 1344, Staphylococcus aureus ATCC 25923 ve Staphylococcus epidermidis DSMZ 20044 mikroorganizmalarına karşı minimum inhibisyon konsantrasyonu (MIC) yöntemi ile belirlenmiştir.

Sonuçlar: GC-MS analizi sonucunda muskat uçucu yağında γ-terpinene (19.16%), (-)-terpinen-4-ol (14.08%), α-pinene (6.75%), myristicin (6.18%), β-pinene (4.85%), sylvestrene (4.72%), elemicin (4.68%) and safrole (4.17%) ana bileşenler olarak tespit edilmiştir. Ayrıca, α-terpinyl acetate (34.55%), eucalyptol (24.91%), (-)-terpinen-4-ol (10.63%), linalool (7.69%) ve neryl butyrate (6.58%) bileşikleri de kakule uçucu yağının ana bileşenleri olarak tespit edilmiştir. Muskat ve kakule uçucu yağlarının deney mikroorganizmalar karşı antimikrobiyal aktiviteleri tespit edilmiştir.

Araştırma vurguları: Uçucu yağın tıp ve gıda endüstrisinde koruyucu olarak kullanılması önerilmiştir.

Keywords

Muskat, Kakule, Uçucu yağ, Antimikrobiyal aktivite

References

• Altuner, E. M., Akata, I., and Canlı, K. (2012). "In vitro Antimicrobial Activity Screening of Bovista nigrescens Pers." Journal of Forestry Faculty of Kastamonu University, 12(1).
• Balouiri, M., Sadiki, M., and Ibnsouda, S. K. (2016). "Methods for in vitro evaluating antimicrobial activity: A review." Journal of Pharmaceutical Analysis, 6(2), 71-79.
• Khan, I. A., and Abourashed, E. A. (2011). Leung's encyclopedia of common natural ingredients: used in food, drugs and cosmetics, John Wiley & Sons.
• Kubo, I., Himejima, M., and Muroi, H. (1991). "Antimicrobial activity of flavor components of cardamom Elettaria cardamomum (Zingiberaceae) seed." Journal of agricultural and food chemistry, 39(11), 1984-1986.
• Narasimhan, B., and Dhake, A. S. (2006). "Antibacterial principles from Myristica fragrans seeds." Journal of medicinal food, 9(3), 395-399.