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The Effects of Cold Stratification, Cold Storage and Gibberellic acid (GA3) on Seed Germination of Bilberry (Vaccinium myrtillus L.)

Year 2021, Volume: 21 Issue: 2, 176 - 182, 30.09.2021
https://doi.org/10.17475/kastorman.1000467

Abstract

Abstract
Aim of study: The effects of cold stratification, cold storage and gibberellic acid (GA3) on seed germination of bilberry (Vaccinium myrtillus L.) were investigated.
Area of study: Ripe berries of V.myrtillus were collected from the Kaz Mountains (İda Mount) National Park-Balıkesir province in Turkey.
Material and methods: In the study five levels of cold moist stratification period, cold dry storage period (control, 15, 30, 60, 90 days) at +4 ºC and three dose of GA3 (control, 500, 1000, 1500 mg/L) treatments were applied. Data were tested independently by ANOVA according to three factors with three replications. 50 seeds were used for each treatment for each replication.
Main results: Cold stratifications, cold storage and GA3 treatments did not increase the germination percentage of bilberry seeds. Germination was found between 23-40% in all experiments.
Highlights: Different pretreatments are advised for increasing germination percentage of V. myrtillus L. seeds

References

  • Aalders, L.E. & Hall, I.V. (1979). Germination of lowbush blueberry seeds as affected by sizing, planting cover, storage, and pelleting. Canadian Journal of Plant Science, 59(2), 527-530.
  • Ballington, J.R, Galletta, G.J. & Pharr, D.M. (1976). Gibberellin effects on rabbiteye blueberry seed germination. HortScience, 11(4), 410-411.
  • Barney, D.L., Shafii, B. & Price, W.J., (2001). Cold stratification delays germination of black huckleberry seeds. HortScience, 36(4), 813.
  • Barney, D.L. (2003). Effects of light, surface sterilization, and fungicides on the germination of black huckleberry seeds. Small Fruits Review, 2(2), 73-80.
  • Baskin, C.C. & Baskin, J.M. (2014). Seeds ecology, biogeography, and evolution of dormancy and germination. Academic Press, San Diego.
  • Baskin, C.C., Milberg, P., Andersson, L. & Baskin, J.M. (2000). Germination studies of three dwarf shrubs (Vaccinium, Ericaceae) of Northern Hemisphere coniferous forests. Can. J. Bot., 78, 1552-1560.
  • Bewley, J.D. (1997). Seed germination and dormancy. Plant Cell, 9, 1055-1066.
  • Bilgin, S., Alım, E. & Çeliktaş Yeşil, Ö. (2011). Some fruit and seed characteristics of bilberry (Vaccinium myrtillus L.) from Kaz Mountains (Mount Ida). In: Efe, R., Öztürk, M., Atalay, İ. (Editors). International Symposium on Kazdağları (Mount Ida) and Edremit, 5-7 May 2011, Edremit, Turkey, 109-119.
  • Bone, K. & Morgan, M. (1997). Bilberry: the vision herb. MediHerb Profess, Rev 59, 14.
  • Ciordia, M., Lucas, De M., Mateos, V., Rodriguez, L, Garcia, J. C. & Majada, J. (2006). Optimization of germination reguirement and seed production of wild-type Vaccinium myrtillus. Proc VIIIth International Symposium on Vaccinium Culture, Eds: Lopes da Fonseca et al. ISHS Acta Hort, 715, 85-90.
  • Davis, P.H. (1988). Flora of Turkey and The Eeast Aegean Islands. Edinburg University Press, Edinburg, UK.
  • Devlin, R.M. & Karczmarczyk, S.J. (1975). Effect of light and gibberellic acid on the germination of 'Early Black' cranberry seeds. Horticultural Research, 15, 19-22.
  • Devlin, R.M. & Karczmarczyk, S.J., (1977). Influence of light and growth regulators on cranberry seed dormancy. Journal of Horticultural Science, 52(2), 283-288.
  • Dweikat, I.M. & Lyrene, P.M. (1988). Adventitious shoot production from leaves of blueberry cultured in vitro. HortScience, 23(3), 629.
  • Dweikat, I.M. & Lyrene, P.M. (1989). Response of highbush blueberry seed germination to gibberellin A3 and 6N-benzyladenine. Can. J. Bot. 67, 3391-3393.
  • Eminağaoğlu, Ö. (2018). Vaccinium L. In: Akkemik, Ü. (Ed.), Türkiye’nin Doğal-Ekzotik Ağaç ve Çalıları, Orman Genel Müdürlüğü Yayınları, Ankara, 278-280.
  • Giba, Z., Grubisic, D. & Konjevic, R., (1993). The effect of white light, growth regulators and temperature on the germination of blueberry (Vaccinium myrtillus L.) seeds. Seed Science and Technology, 21, 521-529.
  • Grieve, M. (1971). A Modern Herbal. New York, NY: Dover Publications, 1, 385-386.
  • Griffin, J.J. & Blazich, F.A., (2002). Vaccinium L. (blueberry or cranberry). North Carolina State University. http://www.nsl.fs.fed.us/wpsm/Vaccinium.pdf (Accessed: 02 10 2010).
  • ISTA, (1996). İnternational Rules of Seed Testing, 1996. Seed Science and Technology, 24, 1-335.
  • Karabulut, B. & Çelik, H. (2013). The Effects of Gibberellin and Dry-cold Stratification on the Germination of Caucasian Whortleberry (Vaccinium arctostaphylos L.) and Bilberry (Vaccinium myrtillus L.) Seeds Collected from Artvin Forest and Plateaus. In: International Caucasian Forestry Symposium, Artvin, Turkey, 24-26 October 2013, 118-1124.
  • Kemper, K. J. (1999). Bilberry (Vaccinium myrtillus). The Longwood Herbal Task Force. http://www.mcp.edu/herbal/bilberry/bilberry.pdf (Accessed: 01 October 2013).
  • Lopez, O.A., Barney, D.L., Shafii, B. & Price, W. J. (2008). Modeling the effects of temperature and gibberellic acid concentration on red huckleberry seed germination. HortScience, 43(1), 223-228. Milbau, A., Graae, B.J., Shevtsova, A. & Nijs, I. (2009). Effects of a warmer climate on seed germination in the subarctic. Annals of Botany 104, 287-296.
  • PFAF, (2020). Vaccinium myrtillus L. https://pfaf.org/user/Plant.aspx?LatinName=Vaccinium+myrtillus (Accessed:5 February 2020)
  • Ranwala, S.M.W. & Naylor, R.E.L. (2010). Production, survival and germination of bilberry (Vaccinium myrtillus L.) seeds. Bot. J. Scotl., 56(1), 55-63.
  • Shahram, S. (2007). Seed dormancy and germination of Vaccinium arctostaphylos L. International Journal of Botany, 3(3), 307-311.
  • Smreciu, A., Pahl, M., Gould, K. & Fung, M. (2008). Native plants for revegetation: Propagation and Establishment. Final Report, prepared for Syncrude Canada, Ltd. and Canadian Oil Sands Network for Research and Development (CONRAD). 25 pp. plus appendices.
  • Taiz, L. & Zeiger, E. (2002). Plant physiology, 3rd ed. Sinauer Associates Publishers, Sunderland, MA.
  • Thakur, K.S. & Rathore, D.S. (1991). Blueberries. In: Mitra SK, Bose TK, Rathore DS (Eds.) Temperate Fruits. Calcutta, India: Horticulture and Allied Publishers, 672-701.
  • Turner, N. (1997). Food plants of interior first peoples. 215p, UBC, Vancouver, BC.
  • Wang, B.S.P. & Berjak, P. (2000). Beneficial effects of moist chilling on the seeds of black spruce (Picea mariana (Mill.) B.S.P.), Annals of Botany, 86, 29-36.
  • Woodward, F.I. (1986). Ecophysiological studies on the shrub Vaccinium myrtillus L. taken from a wide altitudinal range. Oecologia, 70, 580-586.
  • Vander Kloet, S.P. & Hall, I.V. (1981). The Biological Flora of Canada. 2. Vaccinium myrtilloides Michx., velvet-leaf blueberry. Canadian Field-Naturalist, 95(3), 329-345.

Çoban Üzümü (Vaccinium myrtillus L.) Tohumlarının Çimlendirilmesinde Soğuk Katlama, Soğuk Depolama ve Gibberellik asit (GA3) Uygulamalarının Etkisi

Year 2021, Volume: 21 Issue: 2, 176 - 182, 30.09.2021
https://doi.org/10.17475/kastorman.1000467

Abstract

Çalışmanın amacı: Soğuk saklama, soğuk depolama ve gibberellik asidin (GA3) çoban üzümü (Vaccinium. myrtillus L.) tohumlarının çimlenmesi üzerine etkileri araştırılmıştır.
Çalışma alanı: V. myrtillus L.’un olgun meyveleri Türkiye de Balıkesir-Kaz dağları Milli Parkı içerisinden toplanmıştır.
Materyal ve yöntem: Bu çalışmada +4 °C’de beş seviyeli soğuk nemli saklama, soğuk kuru depolama periyodu (kontrol, 15, 30, 60, 90 günler) ve üç doz (kontrol, 500, 1000, 1500 mg/L) GA3 işlemleri uygulanmıştır. Elde edilen veriler, üç tekerrürlü üç faktöre göre birbirinden bağımsız olarak ANOVA ile test edilmiştir. Her bir işlem ve tekerrür için 50 adet tohum kullanılmıştır.
Temel sonuçlar: Soğuk katlama, soğuk depolama ve GA3 işlemleri çoban üzümü tohumlarının çimlenme yüzdesini artırmamıştır. Tüm denemelerde çimlenme oranları %23-40 arasında bulunmuştur.
Araştırma vurguları: V. myrtillus L. tohumlarının çimlenme yüzdesini arttırmak için farklı ön uygulamaların yapılması önerilmektedir.

References

  • Aalders, L.E. & Hall, I.V. (1979). Germination of lowbush blueberry seeds as affected by sizing, planting cover, storage, and pelleting. Canadian Journal of Plant Science, 59(2), 527-530.
  • Ballington, J.R, Galletta, G.J. & Pharr, D.M. (1976). Gibberellin effects on rabbiteye blueberry seed germination. HortScience, 11(4), 410-411.
  • Barney, D.L., Shafii, B. & Price, W.J., (2001). Cold stratification delays germination of black huckleberry seeds. HortScience, 36(4), 813.
  • Barney, D.L. (2003). Effects of light, surface sterilization, and fungicides on the germination of black huckleberry seeds. Small Fruits Review, 2(2), 73-80.
  • Baskin, C.C. & Baskin, J.M. (2014). Seeds ecology, biogeography, and evolution of dormancy and germination. Academic Press, San Diego.
  • Baskin, C.C., Milberg, P., Andersson, L. & Baskin, J.M. (2000). Germination studies of three dwarf shrubs (Vaccinium, Ericaceae) of Northern Hemisphere coniferous forests. Can. J. Bot., 78, 1552-1560.
  • Bewley, J.D. (1997). Seed germination and dormancy. Plant Cell, 9, 1055-1066.
  • Bilgin, S., Alım, E. & Çeliktaş Yeşil, Ö. (2011). Some fruit and seed characteristics of bilberry (Vaccinium myrtillus L.) from Kaz Mountains (Mount Ida). In: Efe, R., Öztürk, M., Atalay, İ. (Editors). International Symposium on Kazdağları (Mount Ida) and Edremit, 5-7 May 2011, Edremit, Turkey, 109-119.
  • Bone, K. & Morgan, M. (1997). Bilberry: the vision herb. MediHerb Profess, Rev 59, 14.
  • Ciordia, M., Lucas, De M., Mateos, V., Rodriguez, L, Garcia, J. C. & Majada, J. (2006). Optimization of germination reguirement and seed production of wild-type Vaccinium myrtillus. Proc VIIIth International Symposium on Vaccinium Culture, Eds: Lopes da Fonseca et al. ISHS Acta Hort, 715, 85-90.
  • Davis, P.H. (1988). Flora of Turkey and The Eeast Aegean Islands. Edinburg University Press, Edinburg, UK.
  • Devlin, R.M. & Karczmarczyk, S.J. (1975). Effect of light and gibberellic acid on the germination of 'Early Black' cranberry seeds. Horticultural Research, 15, 19-22.
  • Devlin, R.M. & Karczmarczyk, S.J., (1977). Influence of light and growth regulators on cranberry seed dormancy. Journal of Horticultural Science, 52(2), 283-288.
  • Dweikat, I.M. & Lyrene, P.M. (1988). Adventitious shoot production from leaves of blueberry cultured in vitro. HortScience, 23(3), 629.
  • Dweikat, I.M. & Lyrene, P.M. (1989). Response of highbush blueberry seed germination to gibberellin A3 and 6N-benzyladenine. Can. J. Bot. 67, 3391-3393.
  • Eminağaoğlu, Ö. (2018). Vaccinium L. In: Akkemik, Ü. (Ed.), Türkiye’nin Doğal-Ekzotik Ağaç ve Çalıları, Orman Genel Müdürlüğü Yayınları, Ankara, 278-280.
  • Giba, Z., Grubisic, D. & Konjevic, R., (1993). The effect of white light, growth regulators and temperature on the germination of blueberry (Vaccinium myrtillus L.) seeds. Seed Science and Technology, 21, 521-529.
  • Grieve, M. (1971). A Modern Herbal. New York, NY: Dover Publications, 1, 385-386.
  • Griffin, J.J. & Blazich, F.A., (2002). Vaccinium L. (blueberry or cranberry). North Carolina State University. http://www.nsl.fs.fed.us/wpsm/Vaccinium.pdf (Accessed: 02 10 2010).
  • ISTA, (1996). İnternational Rules of Seed Testing, 1996. Seed Science and Technology, 24, 1-335.
  • Karabulut, B. & Çelik, H. (2013). The Effects of Gibberellin and Dry-cold Stratification on the Germination of Caucasian Whortleberry (Vaccinium arctostaphylos L.) and Bilberry (Vaccinium myrtillus L.) Seeds Collected from Artvin Forest and Plateaus. In: International Caucasian Forestry Symposium, Artvin, Turkey, 24-26 October 2013, 118-1124.
  • Kemper, K. J. (1999). Bilberry (Vaccinium myrtillus). The Longwood Herbal Task Force. http://www.mcp.edu/herbal/bilberry/bilberry.pdf (Accessed: 01 October 2013).
  • Lopez, O.A., Barney, D.L., Shafii, B. & Price, W. J. (2008). Modeling the effects of temperature and gibberellic acid concentration on red huckleberry seed germination. HortScience, 43(1), 223-228. Milbau, A., Graae, B.J., Shevtsova, A. & Nijs, I. (2009). Effects of a warmer climate on seed germination in the subarctic. Annals of Botany 104, 287-296.
  • PFAF, (2020). Vaccinium myrtillus L. https://pfaf.org/user/Plant.aspx?LatinName=Vaccinium+myrtillus (Accessed:5 February 2020)
  • Ranwala, S.M.W. & Naylor, R.E.L. (2010). Production, survival and germination of bilberry (Vaccinium myrtillus L.) seeds. Bot. J. Scotl., 56(1), 55-63.
  • Shahram, S. (2007). Seed dormancy and germination of Vaccinium arctostaphylos L. International Journal of Botany, 3(3), 307-311.
  • Smreciu, A., Pahl, M., Gould, K. & Fung, M. (2008). Native plants for revegetation: Propagation and Establishment. Final Report, prepared for Syncrude Canada, Ltd. and Canadian Oil Sands Network for Research and Development (CONRAD). 25 pp. plus appendices.
  • Taiz, L. & Zeiger, E. (2002). Plant physiology, 3rd ed. Sinauer Associates Publishers, Sunderland, MA.
  • Thakur, K.S. & Rathore, D.S. (1991). Blueberries. In: Mitra SK, Bose TK, Rathore DS (Eds.) Temperate Fruits. Calcutta, India: Horticulture and Allied Publishers, 672-701.
  • Turner, N. (1997). Food plants of interior first peoples. 215p, UBC, Vancouver, BC.
  • Wang, B.S.P. & Berjak, P. (2000). Beneficial effects of moist chilling on the seeds of black spruce (Picea mariana (Mill.) B.S.P.), Annals of Botany, 86, 29-36.
  • Woodward, F.I. (1986). Ecophysiological studies on the shrub Vaccinium myrtillus L. taken from a wide altitudinal range. Oecologia, 70, 580-586.
  • Vander Kloet, S.P. & Hall, I.V. (1981). The Biological Flora of Canada. 2. Vaccinium myrtilloides Michx., velvet-leaf blueberry. Canadian Field-Naturalist, 95(3), 329-345.
There are 33 citations in total.

Details

Primary Language English
Journal Section Articles
Authors

Serap Bilgin This is me

Esra Alım This is me

Publication Date September 30, 2021
Published in Issue Year 2021 Volume: 21 Issue: 2

Cite

APA Bilgin, S., & Alım, E. (2021). The Effects of Cold Stratification, Cold Storage and Gibberellic acid (GA3) on Seed Germination of Bilberry (Vaccinium myrtillus L.). Kastamonu University Journal of Forestry Faculty, 21(2), 176-182. https://doi.org/10.17475/kastorman.1000467
AMA Bilgin S, Alım E. The Effects of Cold Stratification, Cold Storage and Gibberellic acid (GA3) on Seed Germination of Bilberry (Vaccinium myrtillus L.). Kastamonu University Journal of Forestry Faculty. September 2021;21(2):176-182. doi:10.17475/kastorman.1000467
Chicago Bilgin, Serap, and Esra Alım. “The Effects of Cold Stratification, Cold Storage and Gibberellic Acid (GA3) on Seed Germination of Bilberry (Vaccinium Myrtillus L.)”. Kastamonu University Journal of Forestry Faculty 21, no. 2 (September 2021): 176-82. https://doi.org/10.17475/kastorman.1000467.
EndNote Bilgin S, Alım E (September 1, 2021) The Effects of Cold Stratification, Cold Storage and Gibberellic acid (GA3) on Seed Germination of Bilberry (Vaccinium myrtillus L.). Kastamonu University Journal of Forestry Faculty 21 2 176–182.
IEEE S. Bilgin and E. Alım, “The Effects of Cold Stratification, Cold Storage and Gibberellic acid (GA3) on Seed Germination of Bilberry (Vaccinium myrtillus L.)”, Kastamonu University Journal of Forestry Faculty, vol. 21, no. 2, pp. 176–182, 2021, doi: 10.17475/kastorman.1000467.
ISNAD Bilgin, Serap - Alım, Esra. “The Effects of Cold Stratification, Cold Storage and Gibberellic Acid (GA3) on Seed Germination of Bilberry (Vaccinium Myrtillus L.)”. Kastamonu University Journal of Forestry Faculty 21/2 (September 2021), 176-182. https://doi.org/10.17475/kastorman.1000467.
JAMA Bilgin S, Alım E. The Effects of Cold Stratification, Cold Storage and Gibberellic acid (GA3) on Seed Germination of Bilberry (Vaccinium myrtillus L.). Kastamonu University Journal of Forestry Faculty. 2021;21:176–182.
MLA Bilgin, Serap and Esra Alım. “The Effects of Cold Stratification, Cold Storage and Gibberellic Acid (GA3) on Seed Germination of Bilberry (Vaccinium Myrtillus L.)”. Kastamonu University Journal of Forestry Faculty, vol. 21, no. 2, 2021, pp. 176-82, doi:10.17475/kastorman.1000467.
Vancouver Bilgin S, Alım E. The Effects of Cold Stratification, Cold Storage and Gibberellic acid (GA3) on Seed Germination of Bilberry (Vaccinium myrtillus L.). Kastamonu University Journal of Forestry Faculty. 2021;21(2):176-82.

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