Türkiye'de Abies Taksonlarından Elde Edilen Uçucu Yağların Kimyasal Bileşimi ve Antimikrobiyal Aktivitelerinin Araştırılması

Year 2023, Volume: 23 Issue: 1, 31 - 46, 24.03.2023

Ayşe Öztürk Pulatoğlu Kerim Güney Talip Çeter Esma Sena Yılmaz

https://doi.org/10.17475/kastorman.1269485

Cited By: 1

Abstract

Çalışmanın amacı: Bu çalışmanın amacı, Türkiye’deki Abies taksonlarının yaprak dal ve kozalaklarından elde edilen uçucu yağların biyokimyasal bileşeni ve antimikrobiyal etkilerini ortaya koymaktır.
Materyal ve yöntem: Uçucu yağlar, 19 mikroorganizmaya karşı su buharı distilasyonu metodu ve MİK testi kullanılarak, antimikrobiyal ve antifungal aktiviteleri açısından araştırılmıştır. MİK testine göre etki gözlenen kuyucuklarda, saptanan etkinin bakteri öldürücü (Bakterisidal) ya da gelişmesini durdurucu (Bakteriostatik) olduğunu saptamak için MBK testi yapılmıştır. Kimyasal kompozisyonun tespiti için; clevenger cihazı ile bitkilerden elde edilen uçucu yağlara ait numunelerin GC-MS QP 2010 Ultra (Shimadzu) ile analizleri yapılmıştır.
Temel sonuçlar: A.cilicica subsp. isaurica 'nin kimyasal bileşiklerine bakıldığında taksonun tüm kısımlarında beta-pinene, cis-Ocimene ve Beta-Phellandrene'nin ana bileşenler olduğu bulunmuştur. A. nordmanniana subsp. nordmanniana ve A.cilicica subsp.cilicica taksonlarının dallarında ve yapraklarında uçucu yağ ana bileşenlerinde farklılıklar vardır.
Önemli vurgular: Çalışmanın sonuçları, Abies taksonlarının yaprak dal ve kozalaklarından elde edilen yağların destekleyici sağlık ürünü olarak ve ilave çalışmalarla medikal amaçlı olarak da kullanılabileceğini ortaya koymaktadır.

Keywords

Abies, Antimikrobiyal Aktivite, Biyokimyasal İçerik, Uçucu Yağlar, Türkiye

Chemical Composition of Essential Oils Obtained from Abies taxa in Türkiye and Investigation of Antimicrobial Activities

Abstract

Aim of study: The aim of this study is to reveal the biochemical component and antimicrobial effects of essential oils obtained from different parts of Abies taxa in Türkiye.
Material and methods: Essential oils were analyzed for their antibacterial and antifungal activities by using Steam Distillation Method and MIC test against nineteen microorganisms, In the wells where the effect was observed according to the MIC test, the MBC test was performed to determine that the effect was bactericidal or inhibitory (bacteriostatic). For determination of chemical composition; samples of essential oils obtained from plants with Hydrodistillation were analyzed with GC MS QP 2010 Ultra (Shimadzu).
Main results: Beta-pinene, cis-Ocimene and Beta-Phellandrene were found to be the main components in all parts of the taxon when looking at the chemical compounds of A. cilicica subsp. isaurica taxon. There are differences in essential oil components in the branches and leaves of A. nordmanniana subsp. nordmanniana and A. cilicica subsp. cilicica taxa in the main components.
Highlights: The results of the study reveal that the oils obtained from the leaves, branches and cones of Abies taxa can be used as a supportive health product and for medical purposes with additional studies.

Keywords

Abies, Antimicrobial Activity, Biochemical Content, Essential Oils, Türkiye

References

• Altuner, E.M., Çeter, T., Gür, M., Güney, K., & Kıran, B., et al. (2018). Chemical Composition and Antimicrobial Activities of Cold-Pressed Oils Obtained From Nettle, Radish and Pomegranate Seeds. Kastamonu Üniversitesi Orman Fakültesi Dergisi, 18(3), 236-247.
• Altuner, E.M., Çeter, T. & Işlek, C. (2010). Cilt enfeksiyonlarının geleneksel tedavisinde kullanılan Ononis spinosa L. külünün antifungal etkisinin araştırılması. Mikrobiyoloji Bülteni, 44(4), 633-639.
• Bader, B.M.A., Çeter, T. & Bani, B. (2018). Investigation of Antimicrobial Activity of Gagea dubia. Commun. Fac. Sci. Univ. Ank. Series C; Biology, 27(2), 224-231.
• Bağcı, E., Başer, K.H.C., Kürkçüoğlu, M., Babac, T. & Celik, S. (1999). Study of the essential oil composition of two subspecies of Abies cilicica (Ant. et Kotschy) Carr. from Turkey. Flavour and Fragrance Journal, 14(1), 47-49.
• Bagci, E. & Digrak, M. (1994). Antimicrobial activities of Abies nordmanniana spp. nordmanniana ve A. nordmanniana equi-trojani. Proceedings of XII. National Biology Congresss, 6-8 July 1994, Edirne, Turkey, 227-231
• Bagci, E. & Digrak, M. (1996). Antimicrobial activity of essential oils of some Abies (Fir) species from Turkey. Flavour and Fragrance Journal, 11(4), 251-256.
• Balouiri, M., Sadiki, M. & Ibnsouda, S.K. (2016). Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis, 6(2), 71-79.
• Barbat, C., Rodino, S., Petrache, P., Butu, M. & Butnariu, M. (2013). Microencapsulatıon of The Allelochemıcal Compounds And Study of Theır Release From Dıfferent Products. Digest Journal of Nanomaterials & Biostructures (DJNB), 8(3), 945-953.
• Baytop, A. (1999). Treatment with Medicinal Plants in Turkey. 2nd Ed. Nobel Publishing Houses, Istanbul, Turkey.
• Benli, M., Bingol, U., Geven, F., Guney, K. & Yigit, N. (2008). An Investigation on the antimicrobial activity of some endemic plant species from Turkey. African Journal of Biotechnology, 7(1), 1-5.
• Butnariu, M., Caunii, A. & Putnoky, S. (2012). Reverse phase chromatographic behaviour of major components in Capsicum Annuumextract. Chemistry Central Journal, 6(1), 1-6.
• Butu, M., Butnariu, M., Rodino, S. & Butu, A. (2014a). Study of Zıngıberene From Lycopersıcon Esculentum Fruıt by Mass Spectometry. Digest Journal of Nanomaterials & Biostructures (DJNB), 9(3), 935-941.
• Butu, M., Rodino, S., Butu, A. & Butnariu, M. (2014b). Screenıng of Bıoflavonoıd and Antıoxıdant Actıvıty of Lens Culınarıs Medıkus. Digest Journal of Nanomaterials & Biostructures (DJNB), 9(2), 519-529.
• Canlı, K., Yetgin, A., Akata, I. & Altuner, EM. (2017). Antimicrobial activity and chemical composition screening of Epilobium montanum root. Indian Journal of Pharmaceutical Education and Research, 51(3), 239-243.
• Çelik, E. & Çelik, G.Y. (2007). Bitki uçucu yağlarının antimikrobiyal özellikleri. Orlab On-Line Mikrobiyoloji Dergisi, 5(2), 1-6.
• Çiçek, S. & Çeter, T. (2019). Investigation of the antimicrobial activity of Vitis vinifera L. Boğazkere. Communications Faculty of Sciences University of Ankara Series C Biology, 28(2), 211-224.
• Dayisoylu, K.S., Duman, A.D., Alma, M.H. & Digrak, M. (2009). Antimicrobial activity of the essential oils of rosin. from cones of Abies cilicica subsp. cilicica. African Journal of Biotechnology, 8(19), 5021-5024.
• Digrak, M., Bagci, E., & Alma, M.H. (2002). Antibiotic action of seed lipids from five tree species grown in Turkey. Pharmaceutical Biology, 40(6), 425-428.
• Dığrak, M., İlçim, A. & Alma, M.H. (1999). Antimicrobial activities of several parts of Pinus brutia, Juniperus oxycedrus, Abies cilicia, Cedrus libani and Pinus nigra. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives, 13(7), 584-587.
• Dulger, B., Ceyhan, M., Alitsaous, M. & Ugurlu, E. (1999). Artemisia absinthium L. (Pelin)’un antimikrobiyal aktivitesi. Turkish Journal of Biology, 23(3), 377-384.
• Ekim, T., Koyuncu, M., Vural, M., Duman, H., Aytaç, Z., et al. (2000). Türkiye Bitkileri Kırmızı Kitabı, Eğrelti ve Tohumlu Bitkiler (Red Data Book of Turkish Plants. Pteridophyta and Spermatophyta). Barışçan Ofset.
• Erturk, O. & Demirbag, Z. (2003). Scorzonare mollis Bieb (Compositae) bitkisinin antimikrobiyal aktivitesi. Ekoloji Cevre Dergisi, 12(47), 27-31.
• Güner, A., Aslan, S., Ekim, T., Vural, M. & Babaç, M.T. (edlr.) (2012) Türkiye Bitkileri Listesi (Damarlı Bitkiler). Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını. İstanbul.
• Hafızoglu, H. & Usta, M. (2005). Chemical composition of coniferous wood species occurring in Turkey. Holz Roh-Werkstoff, 63, 83-85.
• Hammer, K.A., Carson, C.F., & Riley, T.V. (1999). Antimicrobial activity of essential oils and other plant extracts. Journal of Applied Microbiology, 86(6), 985-990.
• İlçim, A., Dığrak, M. & Bağcı, E. (1998). Bazı bitki ekstraktlarının antimikrobiyal etkilerinin araştırılması. Turkish Journal of Biology, 22, 119-125.
• Jones, F.A. (1996). Herbs – useful plants. Their role in history andtoday. European Journal of Gastroenterology and Hepatology, 8: 1227-1231.
• Kayacık, H. (1967). Orman ve Park Ağaçlarının Özel Sistematiği, I. Cilt, Gymnospermeae (Açık Tohumlular). Ġ.Ü. Orm. Fak. Yayını No: 1105 / 98, 384s.
• Kelmanson, J.E., Jaeger, A.K. & Van Staden, J. (2000). Zulu medicinal plants with antibacterial activity. Journal of Ethnopharmacol, 69(3), 241–246.
• Kizil, M., Kizil, G., Yavuz, M. & Aytekin, C. (2002a). Antimicrobial activity of resins obtained from the roots and stems of Cedrus libani and Abies cilicia. Applied Biochemistry and Microbiology, 38, 144-146.
• Kizil, M., Kizil, G., Yavuz, M. & Aytekin, Ç. (2002b). Antimicrobial Activity of the Tar Obtained from the Roots and Stems of Pinus brutia. Pharmaceutical Biology, 40(2), 135-138, DOI: 10.1076/phbi.40.2.135.5840
• Lawrence, B.M. (1985). A review of the world production of essential oils (1984). Perfumer & Flavorist, 10(5), 1-16.
• Lawless, J. (1995). The Illustrated Encyclopedia of Essential Oils.Shaftesbury, UK: Element Books Ltd.
• Leven, M., Vanen Berghe, D.A., & Mertens, F. (1979). Medicinal plants and its importance in antimicrobial activity. Planta Medica, 36, 311-321.
• Lis-Balchin, M. & Deans, S.G. (1997). Bioactivity of selected plantessential oils against Listeria monocytogenes. Journal of Applied Bacteriology. 82, 759-762.
• Mishra, A.K. & Dubey, N.K. (1994). Evaluation of some essentialoils for their toxicity against fungi causing deterioration of storedfood commodities. Applied and Environmental Microbiology, 60, 1101-1105.
• Mitrokosta, D., Mitaku, S., Demetzos, C., Harvala, C., Mentis, A., et al. (1993). Bioactive compounds from the buds of Platanus orientalis and isolation of a new kaempferol glycoside. Planta Medica, 59, 517-520.
• Muthoo, M.K. (1997). Forests and Forestry in Turkey; Food and Agriculture Organization of the United Nations (FAO): Ankara, Turkey.
• OGM, (2007). Orman Atlası. Orman Genel Müdürlüğü Yayınları, Ankara.
• Padiyara, P., Inoue, H. & Sprenger, M. (2018). Global governance mechanisms to address antimicrobial resistance. Infectious Diseases: Research and Treatment, 11, 1178633718767887.
• Parvathi, S. & Brindha, R. (2003). Ethnobotanical Medicines of Anaimalai Union, Ancient Science of Life Vol. XXII.
• Pichette, A., Larouche Pierre, L., Lebrun, M. & Legault, J. (2006). Composition and antibacterial activity of Abies balsamea essential oil. Phytotherapy Research, 20(5), 371-373.
• Rawat, R.B.S. & Uniyal, R.C. (2003). National medicinal plants board committed for overall development of the sector. Agro. Bios. Med. Plants, 1, 12-16.
• Reynolds, J.E.F. (1996). Martindale – the Extra Pharmacopoeia 31stedn. London: Royal Pharmaceutical Society of Great Britain.
• Sakagami, H., Kawazoe, N., Komatsu, N., Simpson, A., Nonoyama, M., et al. (1991). Antitumor, antiviral and immunopotentiating activities of pine cone extracts:potential medicinal efficacy of natural and synthetic lignin-related materials. Anticancer Research, 11(2), 881-888.
• Sakar, M.K., Ercil, D. & Engelshowe, R. (1991). Procyanidins in cones of Pinus halepensis. International Journal of Pharmacognosy, 29, 221-224.
• Sakar, K.M., Ercil, D., Tamer, Ü. & Şahin, N. (1996). Abies nordmanniana subsp.equi-trojani ekstrelerinin antimik-robiyal ve sitotoksik aktiviteleri. XI. Bitkisel İlaç Hammad-deleri Toplantisi A.ü. Ecz. Fak. 22-24 Mayıs, Ankara.
• Samfira, I., Rodino, S., Petrache, P., Cristina, R. T., Butu, M., et al. (2015). Characterization and identity confirmation of essential oils by mid infrared absorption spectrophotometry. Digest Journal of Nanomaterials and Biostructures, 10(2), 557-566.
• Schippmann, U., Leaman, D. & Cunnigham, A.B. (2006). Cultivation and wild collection of medicinal and aromatic plants under sustainability aspects. In: Bogers, R.J., Craker, D., Lange, D. (Eds.), Medicinal and Aromatic Plants. Springer, Dordrecht, 17p., Wageningen UR Frontis.
• Souliman, S.I., Kıran, B., Gür, M., Güney, K., Altuner, E.M., et al. (2019). Chemical Composition and Antimicrobial Activities of Cold-Pressed Oils Obtained From Nigella sativa and Prunus amygdalus. Tobruk University Journal of Medicine Science (TUJMS), 2(1), 77-94.
• Sudharameshwari, K. & Radhika, J. (2007). Antibacterial screening of Aegle marmelos, Lawsonia inermis and Albizzia libbeck. Afr. J. Trad. Complem. Altern. Med., 4, 199-204.
• Tanaka, R., Matsunaga, S. & Zasshi, Y. (1999). Terpenoids and steroids from several euphorbiaceae and pinaceae plants. Journal of the Pharmaceutical Society of Japan, 119, 319-339.
• Tumen, I., Hafizoglu, H., Kilic, A., Dönmez, I. E., Sivrikaya, H., et al. (2010). Yields and constituents of essential oil from cones of Pinaceae spp. natively grown in Turkey. Molecules, 15(8), 5797-5806.
• URL-1 (2018). The Plant List. Acccess date: 07.12.2018 http://www.theplantlist.org/tpl1.1/search?q=Abies
• Ulukanli, Z., Karabörklü, S., Bozok, F., Ateş, B., Erdogan, S., et al. (2014). Chemical composition, antimicrobial, insecticidal, phytotoxic and antioxidant activities of Mediterranean Pinus brutia and Pinus pinea resin essential oils. Chinese journal of natural medicines, 12(12), 901-910.
• Vishnoi, S.P., Ghosh, A.K., Debnath, B., Samanta, S., Gayen, S., et al. (2007). Antibacterial activity of Abies webbiana. Fitoterapia, 78, 153-155.
• Xiang, Q.P., Wei, R., Shao, Y.Z., Yang, Z.Y., Wang, X. Q., et al. (2015). Phylogenetic relationships, possible ancient hybridization, and biogeographic history of Abies (Pinaceae) based on data from nuclear, plastid, and mitochondrial genomes. Molecular Phylogenetics and Evolution, 82, 1-14.
• World Health Organization. (2012). Critically important antimicrobials for human medicine. World Health Organization.
• World Health Organization. (2022). WHO establishes the Global Centre for Traditional Medicine in India.
• Yaltırık, F. & Efe, A. (2000). Dendroloji. İ.Ü. Or. Fak. İstanbul.
• Yeşilada, E., Gürbüz, I. & Shibata, H. (1999). Screening of Turkish anti-ulcerogenic folk remedies for anti-Helicobater pylori activity. J Ethnopharmacol, 66, 289-293.