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Türkiye'nin Kuzeydoğusundan Alınan Propolis Örneklerinin Antimikrobiyal, Anti-biyofilm, Anti-swarming, Anti-quorum Sensing Aktiviteleri ve Sitotoksisitesi

Year 2024, Volume: 24 Issue: 2, 158 - 167, 03.10.2024
https://doi.org/10.17475/kastorman.1557416

Abstract

Çalışmanın amacı: Biyolojik aktif moleküller içerdiğinin anlaşılmasıyla birlikte propolis ile ilgili yapılan çalışmaların sayısı son yıllarda artmıştır. Bu çalışmada üç farklı Türkiye propolisinin etanol ekstraktlarının biyolojik aktiviteleri ve sitotoksik özelliklerinin araştırılması amaçlanmıştır.
Materyal ve yöntem: Ekstraktların antimikrobiyal özellikleri 14 mikroorganizma kullanılarak agar kuyucuk ve mikrodilüsyon yöntemleri ile araştırıldı. Anti-quorum sensing aktivitesi için Chromobacter violaceum, anti-swarming ve anti-biyofilm aktivite değerlendirilmesi için Pseudomonas aeruginosa PAO1 izolat kullanıldı. Ekstraktların sitotoksik etkileri MTT yöntemi ile pankreatik tümöral hücre hattı (AR42J), akciğer adenokarsinoma hücre hattı (A549), meme kanseri hücre ahttı (MDA-MB-231) ve normal epitel hücre hattı (Vero) kullanılarak araştırıldı.
Temel sonuçlar: Çalışmaya dahil edilen tüm ekstraktrın 8/14 mikroorganizmaya karşı etkili olduğu tespit edildi. Ayrıca tüm ekstraktlarda anti-quorum sensing aktivitesi görülürken, ekstraktlarda anti-swarming ve anti-biyofilm aktivitesi görülmedi. Tüm ekstraktların hücre hatları üzerine doza bağımlı sitotoksik etki gösterdiği belirlendi.
Araştırma vurguları: Çalışmada kullanılan propolis örneklerinin gösterdiği biyolojik aktivite nedeniyle gelecekte yeni ilaçların geliştirilmesinde rol oynayabileceği düşünülmektedir.

References

  • Aboghrip, M.K., El-Gayar, K.E., Abbas, M.S., Ahmed, A.E., Soliman, A.S. 2023. Some chemical studies on cytotoxicity of antioxidants from propolis extracts against human pancreatic PANC-1 cancer cell. Egyptian Journal of Chemistry. 6(11), 151-161.
  • Akçelik, N., Akçelik, M. 2022. What makes another life possible in bacteria? Global regulators as architects of bacterial biofilms. World Journal of Microbiology and Biotechnology. 38, 236.
  • AlDreini, S., Fatfat, Z., Abou Ibrahim, N., Fatfat, M., Gali-Muhtasib, H., Khalife, H. 2023. Thymoquinone enhances the antioxidant and anticancer activity of Lebanese propolis. World Journal of Clinical Oncology. 14(5), 203-214.
  • Angiolella, L., Sacchetti, G., Efferth, T. 2018. Antimicrobial and antioxidant activities of natural compounds. Evidence-Based Complementary and Alternative Medicine. 2018, 1945179.
  • Balouiri, M., Sadiki, M., Ibnsouda, S.K. 2016. Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis. 6(2), 71-79.
  • Bankova, V. 2005. Chemical diversity of propolis and the problem of standardization. Journal of Ethnopharmacology. 100, 114–117.
  • Belmehdi, O., El Menyiy, N., Bouyahya, A., El Baaboua, A., El Omari, N., Gallo, M., Montesano, D., Naviglio, D., Zengin, G., Senhaji, N.S., Goh, B.H., Abrini, J. 2022. Recent advances in the chemical composition and biological activities of propolis. Food Reviews International. 39(9), 6078-6128.
  • Bouchelaghem, S., Das, S., Naorem, R.S., Czuni, L., Papp, G., Kocsis, M. 2022. Evaluation of total phenolic and flavonoid contents, antibacterial and antibiofilm activities of Hungarian propolis ethanolic extract against Staphylococcus aureus. Molecules. 27(2), 574.
  • Cora, M., Buruk, C.K., Unsal, S., Kaklikkaya, N., Kolayli, S. 2023. Chemical analysis and in vitro antiviral effects of northeast Türkiye propolis samples against HSV-1. Chemistry and Biodiversity. 20(8), e202300669.
  • Demir, S., Aliyazicioglu, Y., Turan, I., Misir, S., Mentese, A., Yaman, S.O., Akbulut, K., Kilinc, K., Deger, O. 2016. Antiproliferative and proapoptotic activity of Turkish propolis on human lung cancer cell line. Nutrition and Cancer. 68(1), 165-172.
  • Denev, P., Kratchanova, M., Ciz, M., Lojek, A., Vasicek, O., Nedelcheva, P., Blazheva, D., Toshkova , R., Gardeva, E., Yossifova, L., Hyrsl, P., Vojtek, L. 2014. Biological activities of selected polyphenol-rich fruits related to immunity and gastrointestinal health. Food Chemistry. 157, 37–44.
  • Forma, E. & Bryś, M. 2021. Anticancer activity of propolis and its compounds. Nutrients. 13(8), 2594.
  • Gapar, N.A.A., Asem, N., Hapit, N.H.A., Mas, M., Omar, E.A. 2023. Phytochemical profiling and anticancer activities of ethanolic and aqueous propolis extract of Trigona apicalis. Journal of Health and Translational Medicine. 2023(1), 141-148.
  • Huang, S., Zhang, C.P., Wang, K., Li, G.Q., Hu, F.L. 2014. Recent advances in the chemical composition of propolis. Molecules. 19, 19610–19623.
  • Kolayli, S., Ureyen Esertas, U.Z., Kara, Y. 2022. The antimicrobial, anti-quorum Sensing, and anti-biofilm activities of ethanolic propolis extracts used as food supplements. Biology Bulletin. 49(Suppl 3), 21-30.
  • McLean, R.J.C., Pierson, L.S., Fuquac, C. 2004. A simple screening protocol for the identification of quorum signal antagonist. Journal of Microbiological Methods. 58(3), 351–360.
  • Mizrahi, J.D., Surana, R., Valle, J.W., Shroff, R.T. 2020. Pancreatic cancer. The Lancet. 395, 2008-2020.
  • Mokrani, M., Zellagui, A., Hadjab, W., Öztürk, M., Ceylen, O., Bensouici, C. 2023. Bioactive molecules from Algerian propolis with therapeutic potential against the oral pathogen Enterococcus faecalis and Chromobacterium violaceum CV026. Nova Biotechnologica et Chimica. 22(1), e1599.
  • Mosmann, T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods. 65, 55-63.
  • Mukaide, K., Shimamura, Y., Masuda, S., Vongsak, B., Kumazawa, S. 2022. Antibacterial and antibiofilm activities of Thailand propolis against Escherichia coli. Natural Product Communications. 17(4).
  • Murray, P.R., Baron, E.J., Pfaller, M.A., Jorgensen, J.H., Yolken, R.H. 2009. Manual of Clinical Microbiology. Volume 1, 9th Edition. ASM Press, Washington, DC. 390-770.
  • Nasirli, F., Daikh, A., Doğan, N.M., Arslan, S., Mutlu, D., Segueni, N. 2023. In vitro evaluation of cytotoxic activity of Algerian propolis against human breast adenocarcinoma (MDA-MB-231) cells and investigation of its potential mechanism of action. Current Bioactive Compounds. 19(1), 28-38.
  • Neto, N.F.O., Bonvicini, J.F.S., Souza, G.L., Santiago, M.B., Veneziani, R.C.S., Ambrósio, S.R., Bastos,J.K., Silva, M.J.B., Martins, C.H.G., Moura, C.C.G., Turrioni, A.P. 2022. Antibacterial activity of Brazilian red propolis and in vitro evaluation of free radical production. Archives of Oral Biology. 143, 105520.
  • Ożarowski, M., Karpiński, T.M. 2023. The effects of propolis on viral respiratory diseases. Molecules. 28(1), 359.
  • Portela-Márquez, M.A., Ruiz Cruz, S., Chaidez-Quiroz, C., Silva-Beltrán, N.P., Morán-Palacio, E.F. 2022. Phenolic compounds, antihemolytic, anti-inflammatory and antibacterial activity of propolis from southern Sonora. Biotecnia. 2022. 24(3), 77-86.
  • Rashid, M.H., Kornberg, A. 2000. In organic polyphosphate is needed for swimming, swarming, and twitching motilities of Pseudomonas aeruginosa. Proceedings of the National Academy of Sciences of the United States of America. 97(9), 4885-4890.
  • Rattray, J.B., Brown, S.P. 2023. Beyond Thresholds: Quorum-sensing as quantitatively varying reaction norms to multiple environmental dimensions. Israel Journal of Chemistry. 63: e202200109.
  • Rice, S.A., Koh, K.S., Queck, S.Y., Labbate, M., Lam, K., Kjelleberg, S. 2005. Biofilm formation and sloughing in Serratia marcescens are controlled by quorum sensing and nutrient cues. Journal of Bacteriology. 187(10), 3477-3485.
  • Shamsuzzaman, Dar A.M., Yaseen, Z., Alam, K., Hussain, A., Gatoo, M.A. 2013. Steroidal pyrimidines: synthesis, characterization, molecular docking studies with DNA and in vitro cytotoxicity. Journal of Molecular Structure. 1045, 62-71.
  • Tatlısulu, S. & Özgör, E. 2023. Identification of Cyprus propolis composition and evaluation of its antimicrobial and antiproliferative activities. Food Bioscience. 51, 102273. Wojtacka, J. 2022. Propolis contra pharmacological interventions in bees. Molecules. 27(15), 4914.
  • Woods, G.L., Brown-Elliott, B.A., Desmond, E.P., Hall, G.S., Heifets, L., Pfyffer, G.E., Ridderhof, J.C., Wallace, R.J., Warren, N.C., Witebsky, F.G. 2003. Susceptibility testing of mycobacteria, nocardiae, and other aerobic actinomycetes; Approved Standard. NCCLS Doc. M24-A, 18, 20-31.
  • Yuan, J., Yuan, W., Guo, Y., Wu, Q., Wang, F., Xuan, H. 2022. Anti-biofilm activities of Chinese poplar propolis essential oil against Streptococcus mutans. Nutrients. 14(16):3290.
  • Zullkiflee, N., Taha, H., Usman, A. 2022. Propolis: Its role and efficacy in human health and diseases. Molecules. 19;27(18), 6120.

Antimicrobial, Anti-biofilm, Anti-swarming, Anti-quorum Sensing Activities, and Cytotoxicity of Propolis Samples from Northeast of Türkiye

Year 2024, Volume: 24 Issue: 2, 158 - 167, 03.10.2024
https://doi.org/10.17475/kastorman.1557416

Abstract

Aim of study: Studies on propolis have increased as it has been revealed that it contains biologically active molecules. In the current study, it was aimed to analyze biological activity, and cytotoxicity of ethanolic extract of three different propolis samples from Türkiye.
Material and methods: The antibacterial activity of the extracts against 14 microorganisms was assessed using the agar well diffusion method and the microdilution method. Chromobacter violeceum was used in quorum-sensing assay, and Pseudomonas aeruginosa PAO1 strain was used in swarming and biofilm assays. Using the MTT test, the cytotoxic effect of the extracts was examined on the lung adenocarcinoma cell line (A549), pancreatic tumoral cell line (AR42J), breast cancer cell line (MDA-MB-231), and normal epithelial cell line (Vero).
Main results: All propolis extracts were effective against 8/14 microorganisms included in the study. While all propolis extracts have shown anti-quorum sensing activity, there was not any anti-swarming and anti-biofilm activity in each sample. It was demonstrated that every propolis sample had a dose-dependent cytotoxic effect on the examined cell lines.
Research highlights: Due to the biological activity shown by the propolis samples included in the study, it is considered that it has the potential to influence the creation of novel medications in the future.

References

  • Aboghrip, M.K., El-Gayar, K.E., Abbas, M.S., Ahmed, A.E., Soliman, A.S. 2023. Some chemical studies on cytotoxicity of antioxidants from propolis extracts against human pancreatic PANC-1 cancer cell. Egyptian Journal of Chemistry. 6(11), 151-161.
  • Akçelik, N., Akçelik, M. 2022. What makes another life possible in bacteria? Global regulators as architects of bacterial biofilms. World Journal of Microbiology and Biotechnology. 38, 236.
  • AlDreini, S., Fatfat, Z., Abou Ibrahim, N., Fatfat, M., Gali-Muhtasib, H., Khalife, H. 2023. Thymoquinone enhances the antioxidant and anticancer activity of Lebanese propolis. World Journal of Clinical Oncology. 14(5), 203-214.
  • Angiolella, L., Sacchetti, G., Efferth, T. 2018. Antimicrobial and antioxidant activities of natural compounds. Evidence-Based Complementary and Alternative Medicine. 2018, 1945179.
  • Balouiri, M., Sadiki, M., Ibnsouda, S.K. 2016. Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis. 6(2), 71-79.
  • Bankova, V. 2005. Chemical diversity of propolis and the problem of standardization. Journal of Ethnopharmacology. 100, 114–117.
  • Belmehdi, O., El Menyiy, N., Bouyahya, A., El Baaboua, A., El Omari, N., Gallo, M., Montesano, D., Naviglio, D., Zengin, G., Senhaji, N.S., Goh, B.H., Abrini, J. 2022. Recent advances in the chemical composition and biological activities of propolis. Food Reviews International. 39(9), 6078-6128.
  • Bouchelaghem, S., Das, S., Naorem, R.S., Czuni, L., Papp, G., Kocsis, M. 2022. Evaluation of total phenolic and flavonoid contents, antibacterial and antibiofilm activities of Hungarian propolis ethanolic extract against Staphylococcus aureus. Molecules. 27(2), 574.
  • Cora, M., Buruk, C.K., Unsal, S., Kaklikkaya, N., Kolayli, S. 2023. Chemical analysis and in vitro antiviral effects of northeast Türkiye propolis samples against HSV-1. Chemistry and Biodiversity. 20(8), e202300669.
  • Demir, S., Aliyazicioglu, Y., Turan, I., Misir, S., Mentese, A., Yaman, S.O., Akbulut, K., Kilinc, K., Deger, O. 2016. Antiproliferative and proapoptotic activity of Turkish propolis on human lung cancer cell line. Nutrition and Cancer. 68(1), 165-172.
  • Denev, P., Kratchanova, M., Ciz, M., Lojek, A., Vasicek, O., Nedelcheva, P., Blazheva, D., Toshkova , R., Gardeva, E., Yossifova, L., Hyrsl, P., Vojtek, L. 2014. Biological activities of selected polyphenol-rich fruits related to immunity and gastrointestinal health. Food Chemistry. 157, 37–44.
  • Forma, E. & Bryś, M. 2021. Anticancer activity of propolis and its compounds. Nutrients. 13(8), 2594.
  • Gapar, N.A.A., Asem, N., Hapit, N.H.A., Mas, M., Omar, E.A. 2023. Phytochemical profiling and anticancer activities of ethanolic and aqueous propolis extract of Trigona apicalis. Journal of Health and Translational Medicine. 2023(1), 141-148.
  • Huang, S., Zhang, C.P., Wang, K., Li, G.Q., Hu, F.L. 2014. Recent advances in the chemical composition of propolis. Molecules. 19, 19610–19623.
  • Kolayli, S., Ureyen Esertas, U.Z., Kara, Y. 2022. The antimicrobial, anti-quorum Sensing, and anti-biofilm activities of ethanolic propolis extracts used as food supplements. Biology Bulletin. 49(Suppl 3), 21-30.
  • McLean, R.J.C., Pierson, L.S., Fuquac, C. 2004. A simple screening protocol for the identification of quorum signal antagonist. Journal of Microbiological Methods. 58(3), 351–360.
  • Mizrahi, J.D., Surana, R., Valle, J.W., Shroff, R.T. 2020. Pancreatic cancer. The Lancet. 395, 2008-2020.
  • Mokrani, M., Zellagui, A., Hadjab, W., Öztürk, M., Ceylen, O., Bensouici, C. 2023. Bioactive molecules from Algerian propolis with therapeutic potential against the oral pathogen Enterococcus faecalis and Chromobacterium violaceum CV026. Nova Biotechnologica et Chimica. 22(1), e1599.
  • Mosmann, T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods. 65, 55-63.
  • Mukaide, K., Shimamura, Y., Masuda, S., Vongsak, B., Kumazawa, S. 2022. Antibacterial and antibiofilm activities of Thailand propolis against Escherichia coli. Natural Product Communications. 17(4).
  • Murray, P.R., Baron, E.J., Pfaller, M.A., Jorgensen, J.H., Yolken, R.H. 2009. Manual of Clinical Microbiology. Volume 1, 9th Edition. ASM Press, Washington, DC. 390-770.
  • Nasirli, F., Daikh, A., Doğan, N.M., Arslan, S., Mutlu, D., Segueni, N. 2023. In vitro evaluation of cytotoxic activity of Algerian propolis against human breast adenocarcinoma (MDA-MB-231) cells and investigation of its potential mechanism of action. Current Bioactive Compounds. 19(1), 28-38.
  • Neto, N.F.O., Bonvicini, J.F.S., Souza, G.L., Santiago, M.B., Veneziani, R.C.S., Ambrósio, S.R., Bastos,J.K., Silva, M.J.B., Martins, C.H.G., Moura, C.C.G., Turrioni, A.P. 2022. Antibacterial activity of Brazilian red propolis and in vitro evaluation of free radical production. Archives of Oral Biology. 143, 105520.
  • Ożarowski, M., Karpiński, T.M. 2023. The effects of propolis on viral respiratory diseases. Molecules. 28(1), 359.
  • Portela-Márquez, M.A., Ruiz Cruz, S., Chaidez-Quiroz, C., Silva-Beltrán, N.P., Morán-Palacio, E.F. 2022. Phenolic compounds, antihemolytic, anti-inflammatory and antibacterial activity of propolis from southern Sonora. Biotecnia. 2022. 24(3), 77-86.
  • Rashid, M.H., Kornberg, A. 2000. In organic polyphosphate is needed for swimming, swarming, and twitching motilities of Pseudomonas aeruginosa. Proceedings of the National Academy of Sciences of the United States of America. 97(9), 4885-4890.
  • Rattray, J.B., Brown, S.P. 2023. Beyond Thresholds: Quorum-sensing as quantitatively varying reaction norms to multiple environmental dimensions. Israel Journal of Chemistry. 63: e202200109.
  • Rice, S.A., Koh, K.S., Queck, S.Y., Labbate, M., Lam, K., Kjelleberg, S. 2005. Biofilm formation and sloughing in Serratia marcescens are controlled by quorum sensing and nutrient cues. Journal of Bacteriology. 187(10), 3477-3485.
  • Shamsuzzaman, Dar A.M., Yaseen, Z., Alam, K., Hussain, A., Gatoo, M.A. 2013. Steroidal pyrimidines: synthesis, characterization, molecular docking studies with DNA and in vitro cytotoxicity. Journal of Molecular Structure. 1045, 62-71.
  • Tatlısulu, S. & Özgör, E. 2023. Identification of Cyprus propolis composition and evaluation of its antimicrobial and antiproliferative activities. Food Bioscience. 51, 102273. Wojtacka, J. 2022. Propolis contra pharmacological interventions in bees. Molecules. 27(15), 4914.
  • Woods, G.L., Brown-Elliott, B.A., Desmond, E.P., Hall, G.S., Heifets, L., Pfyffer, G.E., Ridderhof, J.C., Wallace, R.J., Warren, N.C., Witebsky, F.G. 2003. Susceptibility testing of mycobacteria, nocardiae, and other aerobic actinomycetes; Approved Standard. NCCLS Doc. M24-A, 18, 20-31.
  • Yuan, J., Yuan, W., Guo, Y., Wu, Q., Wang, F., Xuan, H. 2022. Anti-biofilm activities of Chinese poplar propolis essential oil against Streptococcus mutans. Nutrients. 14(16):3290.
  • Zullkiflee, N., Taha, H., Usman, A. 2022. Propolis: Its role and efficacy in human health and diseases. Molecules. 19;27(18), 6120.
There are 33 citations in total.

Details

Primary Language English
Subjects Forest Industry Engineering (Other)
Journal Section Articles
Authors

Merve Cora

Ülkü Zeynep Üreyen Esertaş

İnci Durukan

Early Pub Date September 29, 2024
Publication Date October 3, 2024
Submission Date November 20, 2023
Acceptance Date May 8, 2024
Published in Issue Year 2024 Volume: 24 Issue: 2

Cite

APA Cora, M., Üreyen Esertaş, Ü. Z., & Durukan, İ. (2024). Antimicrobial, Anti-biofilm, Anti-swarming, Anti-quorum Sensing Activities, and Cytotoxicity of Propolis Samples from Northeast of Türkiye. Kastamonu University Journal of Forestry Faculty, 24(2), 158-167. https://doi.org/10.17475/kastorman.1557416
AMA Cora M, Üreyen Esertaş ÜZ, Durukan İ. Antimicrobial, Anti-biofilm, Anti-swarming, Anti-quorum Sensing Activities, and Cytotoxicity of Propolis Samples from Northeast of Türkiye. Kastamonu University Journal of Forestry Faculty. October 2024;24(2):158-167. doi:10.17475/kastorman.1557416
Chicago Cora, Merve, Ülkü Zeynep Üreyen Esertaş, and İnci Durukan. “Antimicrobial, Anti-Biofilm, Anti-Swarming, Anti-Quorum Sensing Activities, and Cytotoxicity of Propolis Samples from Northeast of Türkiye”. Kastamonu University Journal of Forestry Faculty 24, no. 2 (October 2024): 158-67. https://doi.org/10.17475/kastorman.1557416.
EndNote Cora M, Üreyen Esertaş ÜZ, Durukan İ (October 1, 2024) Antimicrobial, Anti-biofilm, Anti-swarming, Anti-quorum Sensing Activities, and Cytotoxicity of Propolis Samples from Northeast of Türkiye. Kastamonu University Journal of Forestry Faculty 24 2 158–167.
IEEE M. Cora, Ü. Z. Üreyen Esertaş, and İ. Durukan, “Antimicrobial, Anti-biofilm, Anti-swarming, Anti-quorum Sensing Activities, and Cytotoxicity of Propolis Samples from Northeast of Türkiye”, Kastamonu University Journal of Forestry Faculty, vol. 24, no. 2, pp. 158–167, 2024, doi: 10.17475/kastorman.1557416.
ISNAD Cora, Merve et al. “Antimicrobial, Anti-Biofilm, Anti-Swarming, Anti-Quorum Sensing Activities, and Cytotoxicity of Propolis Samples from Northeast of Türkiye”. Kastamonu University Journal of Forestry Faculty 24/2 (October 2024), 158-167. https://doi.org/10.17475/kastorman.1557416.
JAMA Cora M, Üreyen Esertaş ÜZ, Durukan İ. Antimicrobial, Anti-biofilm, Anti-swarming, Anti-quorum Sensing Activities, and Cytotoxicity of Propolis Samples from Northeast of Türkiye. Kastamonu University Journal of Forestry Faculty. 2024;24:158–167.
MLA Cora, Merve et al. “Antimicrobial, Anti-Biofilm, Anti-Swarming, Anti-Quorum Sensing Activities, and Cytotoxicity of Propolis Samples from Northeast of Türkiye”. Kastamonu University Journal of Forestry Faculty, vol. 24, no. 2, 2024, pp. 158-67, doi:10.17475/kastorman.1557416.
Vancouver Cora M, Üreyen Esertaş ÜZ, Durukan İ. Antimicrobial, Anti-biofilm, Anti-swarming, Anti-quorum Sensing Activities, and Cytotoxicity of Propolis Samples from Northeast of Türkiye. Kastamonu University Journal of Forestry Faculty. 2024;24(2):158-67.

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