Xanthoparmelia pulla'nın (Ach.) O. Blanco, A. Crespo, Elix, D. Hawksw. & Lumbsch'un (Parmeliaceae) Terapötik Potansiyelinin Araştırılması

Year 2025, Volume: 25 Issue: 1, 9 - 19, 26.03.2025

Sena Kiymik Mustafa Kocakaya , Selen İlgün , Gökçe Şeker Karatoprak , Zekiye Kocakaya , Mehmet Çadır

https://doi.org/10.17475/kastorman.1660492

Abstract

Çalışmanın amacı: Bu çalışma, Türkiye'de bulunan X. pulla türünün metanol ekstresinin antioksidan etkisi ve potansiyel toksisitesini araştırmayı ve bu türün sekonder metabolitlerini tanımlamayı amaçlamaktadır.
Çalışma alanı: Örnekler Yozgat Bozok Üniversitesi Doğu Kampüsü'nden toplanmıştır.
Materyal ve yöntem: X. pulla'daki sekonder metabolitlerin tespiti HPLC kullanılarak gerçekleştirilmiştir. Antioksidan aktivite, DPPH● ve ABTS●+ yöntemleri kullanılarak değerlendirilmiştir. Sitotoksisite, MCF-7 ve MDA-MB-231 hücre hatlarında MTT deneyi kullanılarak değerlendirildi.
Temel sonuçlar: En yüksek sekonder metabolit salazinik asit (37.23±9.21 mg/ekstre) olarak tespit edilmiştir. Toplam fenol içeriği 172.77±10.50 mg GAE/g, flavonoid içeriği ise 9.25±1.32 mg CA/g’dır. Ekstre, 2 mg/mL konsantrasyonda %87.41 DPPH● radikal süpürme aktivitesi göstermiş, ABTS●+ radikalini ise tüm konsantrasyonlarda etkili şekilde nötralize etmiştir. Sitotoksisite açısından, 125 μg/mL’de MCF-7 hücre canlılığı %66.84, 250 μg/mL’de MDA-MB-231 hücre canlılığı %39.42 oranında inhibe edilmiştir.
Araştırma vurguları: Bu çalışma, X. pulla'nın biyolojik aktivitesi ve doğal ürün bazlı ilaç geliştirme potansiyelini ortaya koymaktadır.

Keywords

Xanthoparmelia pulla, HPLC, Salazinic acid, Antioksidan, Sitotoksisite

Exploring The Therapeutic Potential of Xanthoparmelia pulla (Ach.) O. Blanco, A. Crespo, Elix, D. Hawksw. & Lumbsch (Parmeliaceae)

Abstract

Aim of study: This study aims to investigate the antioxidant effect and potential toxicity of the methanol extract of X. pulla species in Turkey and to identify the secondary metabolites of this species.
Area of study: Samples were collected from Yozgat Bozok University East Campus.
Material and method: Detection of secondary metabolites in X. pulla was performed using HPLC. Antioxidant activity was evaluated using DPPH● and ABTS●+ methods. Cytotoxicity was evaluated using MTT assay in MCF-7 and MDA-MB-231 cell lines.
Main results: The highest secondary metabolite was salazinic acid (37.23±9.21 mg/extract). Total phenol content was 172.77±10.50 mg GAE/g and flavonoid content was 9.25±1.32 mg CA/g. The extract showed 87.41% DPPH● radical scavenging activity at 2 mg/mL concentration and effectively neutralized ABTS●+ radical at all concentrations. In terms of cytotoxicity, MCF-7 cell viability was inhibited by 66.84% at 125 μg/mL and MDA-MB-231 cell viability was inhibited by 39.42% at 250 μg/mL.
Research highlights: This study reveals the biological activity of X. pulla and its potential for natural product based drug development.

Keywords

Xanthoparmelia pulla, HPLC, Salazinic acid, Antioxidant, Cytotoxicity

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