Determination of Antimicrobial and Antioxidant Activity of Atropa belladonna L.

Year 2025, Volume: 25 Issue: 2, 188 - 199, 30.09.2025

Merve Ülkü Sayım , Rüveyde Pala , Cenker Yaman , Gizem Gül , Atakan Benek , Dilay Turu , Mustafa Eray Bozyel , Kerem Canlı

https://doi.org/10.17475/kastorman.1787316

Abstract

Aim of study: Bacteria that have developed resistance to antibiotics have become one of the major challenges facing global healthcare systems. This study evaluates the antimicrobial and antioxidant effects of Atropa belladonna extracts against an extended variety of microbial strains, addressing the urgent need due to increasing resistance.
Material and method: In this study, disc diffusion, minimum inhibitory concentration testing, and DPPH antioxidant analyses were conducted on the Atropa belladonna plant.
Main results: Using the ethanol extract, antimicrobial activity was tested against 26 bacterial and one yeast strain by disc diffusion, showing inhibitory effects on six microorganisms (four standard strains and two food isolates). MIC assays identified activity against Bacillus subtilis DSMZ 1971, Candida albicans DSMZ 1386, and Enterococcus durans. The DPPH assay demonstrated the greatest antioxidant activity at 200 mg/mL concentration, reaching 71.34%.
Research highlights: The study results emphasize the need to consider Atropa belladonna’s antimicrobial and antioxidant capacities. Therefore, the study should be combined with various doses, strains, or methods.

Keywords

Atropa belladonna L. , Disc diffusion Test , Minimum Inhibition Concentration , DPPH Test

Atropa belladonna L.’nın Antimikrobiyal ve Antioksidan Aktivitesinin Belirlenmesi

Abstract

Çalışmanın Amacı: Antibiyotiğe dirençli bakterilerin artan yaygınlığı günümüzde insan sağlığı için önemli bir tehdit oluşturmaktadır. Çalışmamız, antibiyotiğe dirençli bakterilerin oluşturduğu artan tehdidi göz önünde bulundurarak Atropa belladonna bitkisinden elde edilen ekstraktın antimikrobiyal ve antioksidan potansiyelini daha geniş bir suş yelpazesinde değerlendirmeyi amaçlamaktadır.
Materyal ve yöntem: Bu çalışmada, Atropa belladonna bitkisi üzerinde disk difüzyon, minimum inhibisyon konsantrasyon testi ve DPPH antioksidan analizleri yapılmıştır.
Temel sonuçlar: Disk difüzyon testinde, bitkinin etanol özütü 26 bakteri ve 1 mayaya karşı test edilmiştir. Sonuç olarak, toplam 6 mikroorganizmada, 4 standart (ST) ve 2 gıda izolatında (FI) etkiler gözlenmiştir. Minimum inhibisyon konsantrasyon testinde, Bacillus subtilis DSMZ 1971, Candida albicans DSMZ 1386 ve Enterococcus durans (FI) üzerinde etki belirlenmiştir. DPPH testinde elde edilen sonuçlara göre, en yüksek antioksidan aktivite 200 mg/mL konsantrasyonda %71.343 olarak kaydedilmiştir.
Araştırma vurguları: Çalışma sonuçları, Atropa belladonna'nın antimikrobiyal ve antioksidan kapasitelerinin dikkate alınması gerektiğini vurgulamaktadır. Bu sebeple çalışma çeşitli doz, suş veya metotlarla kombine edilmelidir.

Keywords

Atropa belladonna L. , Disk Difüzyon Testi , Minimum İnhibisyon Konsantrasyon Testi , DPPH Testi

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