İsatinin İskemi ve Reperfüzyon Hasarı Üzerine Etkisi: İzole Kalpte Deneysel Bir Çalışma

Abstract

Giriş: İsatin, vücut sıvılarında endojen olarak bulunan, sinir sisteminde koruyucu, antikonvülzan ve sedatif rolü olduğu bildirilen bir indoldür. Bu çalışmanın amacı, izole sıçan kalbinde oluşturulan iskemi reperfüzyon modelinde isatinin sol ventrikül işlevi üzerine etkisini incelemektir.

Hastalar ve Yöntem: Erkek Wistar türü sıçanlar dört gruba ayrıldı. I ve I-atriyal natriüretik peptid (ANP) gruplarına kalpler Langendoff düzeneğine yerleştirilmeden önce isatin [50 mg/kg intraperitoneal (ip)] verildi. C ve ANP gruplarına serum fizyolojik verildi. Tüm gruplara düşük akımlı iskemi uygulandı. İskeminin 15 dakika öncesinde, ANP ve I + ANP gruplarında perfüzyon solüsyonuna ANP (0.1 μM/L) eklendi. İskemi öncesi ve sonrasında sol ventrikül gelişim basıncı (LVDP) ve maksimum ve minimum basınç değişimleri kaydedildi. Tüm gruplarda perfüzyon sıvısından siklik guanozin monofosfate (cGMP) düzeyleri ölçüldü.

Bulgular: Reperfüzyonun 60. dakikasında I grubunun LVDP ve mimimum basınç değişim değerleri C grubuna benzer bulundu. İskemi öncesinde ve sonrasında, I grubunun cGMP değerleri C grubuna benzer ancak ANP ve I + ANP gruplarından daha düşük bulundu.

Sonuç: İskemi öncesinde isatin verilmesi sıçan kalbinde kardiyak işlevi anlamlı şekilde değiştirmemektedir. Bu çalışmanın bulguları, isatinin düşük akımlı iskemi sonrasında kardiyak işlevi bozucu etki oluşturmadığı yönündedir.

Keywords

• İsatin,atriyal natriüretik peptid,iskemi,cGMP

Effect of Isatin on Ischemia and Reperfusion Injury: an Experimental Study in the Isolated Rat Heart

Abstract

Introduction: Isatin is an endogen indole that is found in body fluids and reported as a neuroprotective, anticonvulsant, and sedative agent. The aim of the present study was to investigate the effects of isatin on left ventricle functions before and after low-flow ischemia in the isolated rat heart.

Patients and Methods: Male Wistar rats were divided into four groups. Isatin (I, 50 mg/kg intraperitoneally) was administered in the I and I-atrial natriuretic peptide (ANP) groups 20 min before the hearts were placed on the Langendorff apparatus. Serum physiologic was administered to the control (C) and ANP groups. Low-flow ischemia was applied in all groups. ANP (0.1 μM/L) was added to perfusion solution in the ANP and I + ANP groups 15 min before ischemia. Left ventricular developed pressure (LVDP) and maximum and minimum pressure changes were recorded before and after ischemia. Cyclic guanosine monophosphate (cGMP) levels were measured in the perfusate in all groups.

Results: LVDP and minimum pressure change values of the I group were found to be similar to the C group at 60 min of reperfusion. The level of cGMP in the I group was similar to the C group but lower than the ANP and I + ANP groups before and after ischemia.

Conclusion: The administration of isatin prior to cardiac ischemia does not significantly alter cardiac function during the reperfusion period in rat heart. The results of the present study showed that isatin may not appear to have a disturbing effect on cardiac functions after low-flow ischemia.

Keywords

• Isatin,atrial natriuretic peptide,ischemia,cGMP

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