Cytotoxin Associated Gene A+ Helicobacter pylori Infection is Related with Endothelial Dysfunction in Normotensive Individuals

Year 2014, Volume: 17 Issue: 1, 1 - 6, 01.01.2013

Aydın Akyüz Rafet Mete Mustafa Oran Şeref Alpsoy Dursun Çayan Akkoyun Pelin Osanmaz Değirmenci Okan Avcı

https://doi.org/10.4274/khj.6448

Abstract

OBJECTIVE: Cytotoxin associated gene (Cag) A+ Helicobacter pylori (HP) is the most virulent HP strain, and its association with endothelial dysfunction remains unclear. Thus we investigated the effects of CagA+ HP on endothelial dependent dilatation and its relationship with C-reactive protein (CRP) in normotensive subjects. METHODS: Sixty individuals with CagA+ HP infection were compared with 40 individuals with CagA(-) HP infection and 50 individuals with no infection in terms of flow-mediated dilatation (FMD), non-endothelium-dependent dilatation (NED) and CRP.RESULTS: In the CagA+ subjects the mean percentage of FMD was 6.3±2.7, which was significantly lower than that of the CagA- subjects (9.8±3.3, p

Keywords

Cytotoxine associated gene A protein, Helicobacter pylori; flow-mediated dilatation

Sitotoksin İlişkili A+ Genli Helicobacter pylori Enfeksiyonu Normotansif Bireylerde Endotelyal Disfonksiyon ile İlişkilidir

Abstract

AMAÇ: Sitotoksin ilişkili A genli Helicobacter pylori (SİAGHP), en çok virülansı olan H. pylori tipidir ve halen endotelyal disfonksiyon ile ilişkisi belirsizdir. Bu nedenle biz SİAGHP'nin endotelyal bağımlı dilatasyon üzerine etkilerini ve C-reaktif protein (CRP) düzeyleri ile ilişkisini normotansif bireylerde araştırdık.YÖNTEMLER: SİAGHP enfeksiyonu olan 60 kişi, sitotoksin ilişkili A geni negatif H. pylori (SAİGNHP) enfeksiyonlu 40 kişi ve enfeksiyonu olmayan 50 kişi ile akım aracılı dilatasyon (AAD), endotele bağımlı olmayan dilatasyon (EBOD) ve CRP düzeyleri yönünden karşılaştırıldı.BULGULAR: SİAGHP'li bireylerde ortalama AAD'nin yüzdesi (6.3±2.7) SİAGNHP olanlardan (9.8±3.3, p

Keywords

Sitotoksin ilişkili gen A protein, Helicobacter pylori; akım aracılı dilatasyon

References

• Cesari M, Penninx BW, Newman AB, Kritchevsky SB, Nicklas BJ, Sutton-Tyrrell K, et al. Inflammatory markers and onset of cardiovascular events: results from the Health ABC study. Circulation 2003;108:2317-22.
• Morrone G, Ciliberto G, Oliviero S. Recombinant interleukin 6 regulates the transcriptional activation of a set of human acute phase genes. J Biol Chem 1988;263:12554-8.
• Franceschi F, Sepulveda AR, Gasbarrini A, Pola P, Silveri NG, Gasbarrini G, et al. Cross-reactivity of anti-CagA antibodies with vascular wall antigens: possible pathogenic link between Helicobacter pylori infection and atherosclerosis. Circulation 2002;106:430-4.
• Gattone M, Iacoviello L, Colombo M, Castelnuovo AD, Soffiantino F, Gramoni A, et al. Chlamydia pneumoniae and cytomegalovirus seropositivity, inflammatory markers, and the risk of myocardial infarction at a young age. Am Heart J 2001;142:633-40.
• Keller TT, Mairuhu AT, De Kruif MD, Klein SK, Gerdes VE, Ten Cate H, et al. Infections and endothelial cells. Cardiovasc Res 2003;60:40- 8.
• Brown LM. Helicobacter pylori: epidemiology and routes of transmission. Epidemiol Rev 2000;22:283-97.
• Huang B, Chen Y, Xie Q, Lin G, Wu Y, Feng Y, et al. CagA-positive Helicobacter pylori strains enhanced coronary atherosclerosis by increasing serum OxLDL and HsCRP in patients with coronary heart disease. Dig Dis Sci 2011;56:109-14.
• Pasceri V, Patti G, Cammarota G, Pristipino C, Richichi G, Di Sciascio G. Virulent strains of Helicobacter pylori and vascular diseases: a meta- analysis. Am Heart J 2006;151:1215-22.
• Jafarzadeh A, Esmaeeli-Nadimi A, Nemati M, Tahmasbi M, Ahmadi P. Serum concentrations of Helicobacter pylori IgG and the virulence factor CagA in patients with ischaemic heart disease. East Mediterr Health J 2010;16:39-44.
• Niccoli G, Franceschi F, Cosentino N, Giupponi B, De Marco G, Merra G, et al. Coronary atherosclerotic burden in patients with infection by CagA- positive strains of Helicobacter pylori. Coron Artery Dis 2010;21:217-21.
• Vanhoutte PM. Endothelial dysfunction: the first step toward coronary arteriosclerosis. Circ J 2009;73:595-601.
• Anderson TJ, Uehata A, Gerhard MD, Meredith IT, Knab S, Delagrange D, et al. Close relation of endothelial function in the human coronary and peripheral circulations. J Am Coll Cardiol 1995;26:1235-41.
• Yudkin JS, Stehouwer CD, Emeis JJ, Coppack SW. C-reactive protein in healthy subjects: associations with obesity, insulin resistance, and endothelial dysfunction: a potential role for cytokines originating from adipose tissue? Arterioscler Thromb Vasc Biol 1999;19:972-8.
• Möhlenkamp S, Lehmann N, Moebus S, Schmermund A, Dragano N, Stang A, et al. Heinz Nixdorf Recall Study Investigators. Quantification of coronary atherosclerosis and inflammation to predict coronary events and all-cause mortality. J Am Coll Cardiol 2011;57:1455-64.
• Jin SW, Her SH, Lee JM, Yoon HJ, Moon SJ, Kim PJ, et al. The association between current Helicobacter pylori infection and coronary artery disease. Korean J Intern Med 2007;22:152-6.
• Strachan DP, Mendall MA, Carrington D, Butland BK, Yarnell JW, Sweetnam PM, et al. Relation of Helicobacter pylori infection to 13-year mortality and incident ischemic heart disease in the caerphilly prospective heart disease study. Circulation 1998;98:1286-90.
• Pasceri V, Cammarota G, Patti G, Cuoco L, Gasbarrini A, Grillo RL, et al. Association of virulent Helicobacter pylori strains with ischemic heart disease. Circulation 1998;97:1675-9.
• Rožanković PB, Huzjan AL, Cupić H, Benčić IJ, Bašić S, Demarin V. Influence of CagA-positive Helicobacter pylori strains on atherosclerotic carotid disease. J Neurol 2011;258:753-61.
• Oshima T, Ozono R, Yano Y, Oishi Y, Teragawa H, Higashi Y, et al. Association of Helicobacter pylori infection with systemic inflammation and endothelial dysfunction in healthy male subjects. J Am Coll Cardiol 2005;45:1219-22.
• Tobin NP, Henehan GT, Murphy RP, Atherton JC, Guinan AF, Kerrigan SW, et al. Helicobacter pylori-induced inhibition of vascular endothelial cell functions: a role for VacA-dependent nitric oxide reduction. Am J Physiol Heart Circ Physiol 2008;295:H1403-13.
• Park JB, Charbonneau F, Schiffrin EL. Correlation of endothelial function in large and small arteries in human essential hypertension. J Hypertens 2001;19:415-20.
• Hink U, Li H, Mollnau H, Oelze M, Matheis E, Hartmann M, et al. Mechanisms underlying endothelial dysfunction in diabetes mellitus. Circ Res 2001;88:E14-2.
• Heitzer T, Ylä-Herttuala S, Luoma J, Kurz S, Münzel T, Just H, et al. Cigarette smoking potentiates endothelial dysfunction of forearm resistance vessels in patients with hypercholesterolemia. Role of oxidized LDL. Circulation 1996;93:1346-53.
• Chimienti G, Russo F, Lamanuzzi BL, Nardulli M, Messa C, Di Leo A, et al. Helicobacter pylori is associated with modified lipid profile: impact on Lipoprotein(a). Clin Biochem 2003;36:359-65.
• Sung JJ, Sanderson JE. Hyperhomocysteinaemia, Helicobacter pylori, and coronary heart disease. Heart 1996;76:305-7.
• Al-Shaer MH, Raghuveer G, Browning R, Sinkey CA, Chenard C, Stumbo P, et al. Effect of hyperhomocysteinemia induced by methionine administration on flow-mediated dilatation of the brachial artery in healthy subjects. Am J Cardiol 2005;95:428-30.
• Yoshino N, Adachi K, Takashima T, Miyaoka Y, Yuki T, Ishihara S, et al. Helicobacter pylori infection does not affect the serum level of homocysteine. Am J Gastroenterol 2002;97:2927-8.