The Use of HPLC-DAD Method in Determining the Quality of Honeys: 5-Hydroxymethylfurfural (HMF) Analysis

Year 2024, , 41 - 45, 15.12.2024

Merve Özbay , Fatma Nur Arslan , Gazi Görür

https://doi.org/10.55213/kmujens.1529151

Abstract

The recognition of 5-hydroxymethylfurfural (HMF) content in honeys has been the subject of many scientific studies as it is a significant indicator of the toxicity, its processing method, storage situations and age. In this research, the amount of HMF in 95 different flower and honeydew honey samples obtained from markets, factory sales offices and local beekeepers in Turkiye was determined. High performance liquid chromatography (HPLC) method was employed to recognize HMF contents. Before sample analysis, the standard HMF solutions were prepared at different concentrations and method validation studies were carried out. The HMF content limit value of flower and honeydew honey in the Honey Communiqué (TFC/Communiqué No:2020/7) is stated as 40 mg.kg-1. According to the obtained results, the HMF contents in honey samples were found as 0.00-16.65 mg.kg-1. It was determined that the amounts of HMF were at the highest level in monofloral milk vetch honey (MH_MV) samples coded MH_MV3 and MH_MV4. Therefore, it was concluded that all of the honey samples were compatible with the Honey Communiqué in terms of HMF and had appropriate honey quality values.

Keywords

Floral honey, Honeydew honey, 5-hydroxymethylfurfural, HPLC

Ethical Statement

All authors declare that they have no further conflict of interest.

Supporting Institution

University of Karamanoglu Mehmetbey

Project Number

220O089 (TÜBİTAK–1002)

Thanks

The research was financially supported by the project with a number of 220O089 (TUBITAK–1002).

Balların Kalitesinin Belirlenmesinde HPLC-DAD Metodunun Kullanımı: 5-Hidroksimetilfurfural (HMF) Analizi

Abstract

Baldaki 5-hidroksimetilfurfural (HMF) içeriğinin tespiti balın toksititesinin, işlenme şeklinin, saklama koşullarının ve yaşının önemli bir göstergesi olduğu için birçok bilimsel araştırmanın konusu olmuştur. Bu çalışmada Türkiye’deki marketlerden, fabrika satış ofislerinden ve yerel bal üreticilerinden temin edilen 95 farklı çiçek ve salgı balı numunesinin HMF içeriği belirlenmiştir. HMF içeriklerinin tespitinde yüksek performanslı sıvı kromatografi (HPLC) yöntemi uygulanmıştır. Numune analizleri öncesinde farklı konsantrasyonlarda standart HMF çözeltileri hazırlanmış ve yönteme ait metot validasyon çalışmaları gerçekleştirilmiştir. Bal Tebliğinde (TGK/Tebliğ No:2020/7) çiçek ve salgı ballarında HMF içeriği sınır değeri 40 mg.kg-1 şeklinde belirtilmektedir. Gerçekleştirdiğimiz analizlerden elde edilen sonuçlara göre numunelerdeki HMF değerleri 0.00-16.65 mg.kg-1şeklindedir. MH_MV3 ve MH_MV4 kodlu monofloral geven balı numunelerinde HMF değerinin en yüksek düzeyde olduğu belirlenmiştir. Sonuç olarak incelenen bal numunelerinin tümünün HMF açısından tebliğ ile uyumlu olduğu ve uygun bal kalite değerlerine sahip oldukları kanaatine varılmıştır.

Keywords

Çiçek balı, Salgı balı, 5-hidroksimetilfurfural, HPLC

Ethical Statement

Tüm yazarlar başka çıkar çatışması olmadığını beyan eder.

Supporting Institution

Karamanoğlu Mehmetbey Üniversitesi

Project Number

220O089 (TÜBİTAK–1002)

Thanks

Araştırma, 220O089 (TÜBİTAK–1002) numaralı proje tarafından finansal olarak desteklenmiştir.

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